Vitellogenin and vitellin from the blowfly Calliphora vicina: Occurrence, purification and antigenic characterization

Correlation between ovarian growth, vitellogenin titer, and yolk polypeptide pattern in the haemolymph ofCalliphora vicina

“…The antivitellogenin (0.86%) was the same as used by Jensen et al 13. Rocket immunoelectrophoresis 14 was used for determination of the vitellogenin levels in the haemolymph samples.…”

Adenotrophic Viviparity in Tsetse Flies: Potential for Population Control and as an Insect Model for Lactation

“…However, tsetse is unique in that it carries only a single yolk protein gene orthologous to Drosophila yp2 (6). In addition to the reduction in yolk protein genes, the tissue specificity of tsetse yolk protein genes differs from that of most brachyceran flies such as Drosophila , in which yolk proteins are produced by both the fat body and ovarian follicle cells (20, 46, 64, 67, 142). In tsetse, yolk protein is synthesized only by the follicle cells of the developing oocyte (6, 58, 62), as in Stomoxys calcitrans (29, 60).…”

Expression of the vitellogenin genes in insects

“…Three polypeptides of 46,000, 45,000, and 44,OOO daltons corresponding to the primary product of synthesis are found in translation of isolated mRNA [Bownes and Hames, 1978;Postlethwait and Kashnitz, 1978;Warren et al, 19791. The vitellogenins of Calliphora erythrocephala and the other Diptera species also contain subunits of 45,000 to 50,000 daltons, but the number of subunits differs with the species [Srdic et al, 1978;Jensen et al, 1981;Fourney et al, 19821. The molecular weight of the native vitellogenin and its subunit relationship in Diptera have not yet been clearly determined because of unavoidable streaking and dissociation during electrophoresis.…”