Vitellogenins in the spider Parasteatoda tepidariorum – expression profile and putative hormonal regulation of vitellogenesis

Bednarek, Agata; Sawadro, Marta; Nicewicz, Łukasz; Babczyńska, Agnieszka

doi:10.1186/s12861-019-0184-x

Cited by 11 publications

(10 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the most ubiquitous genes present in arthropod ovaries that is likely regulated by these zinc-finger transcription factors is vitellogenin, which is used in the process of oocyte development [66]. Our results indicate that these genes are also undergoing alternative splicing, as we find 184 transcripts of the vitellogenin gene, a pattern found in arthropods, and more specifically in other arachnids [19,[86][87][88][89]. Vitellogenesis involves the synthesis and accumulation of proteins for eggs to develop [66].…”

Section: Western Black Widow Spider Genes Associated With Fecunditysupporting

confidence: 53%

Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider

Miles

Ayoub

Garb

et al. 2020

Genes

View full text Add to dashboard Cite

Due to their abundance and ability to invade diverse environments, many arthropods have become pests of economic and health concern, especially in urban areas. Transcriptomic analyses of arthropod ovaries have provided insight into life history variation and fecundity, yet there are few studies in spiders despite their diversity within arthropods. Here, we generated a de novo ovarian transcriptome from 10 individuals of the western black widow spider (Latrodectus hesperus), a human health pest of high abundance in urban areas, to conduct comparative ovarian transcriptomic analyses. Biological processes enriched for metabolism—specifically purine, and thiamine metabolic pathways linked to oocyte development—were significantly abundant in L. hesperus. Functional and pathway annotations revealed overlap among diverse arachnid ovarian transcriptomes for highly-conserved genes and those linked to fecundity, such as oocyte maturation in vitellogenin and vitelline membrane outer layer proteins, hormones, and hormone receptors required for ovary development, and regulation of fertility-related genes. Comparative studies across arachnids are greatly needed to understand the evolutionary similarities of the spider ovary, and here, the identification of ovarian proteins in L. hesperus provides potential for understanding how increased fecundity is linked to the success of this urban pest.

show abstract

Section: Western Black Widow Spider Genes Associated With Fecunditysupporting

confidence: 53%

Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider

Miles

Ayoub

Garb

et al. 2020

Genes

View full text Add to dashboard Cite

show abstract

“…Such dependence may indicate the role of these substances in physiological processes that take place very intensively during that period. Among them, vitellogenesis is the essential process; it begins in spiders in the last nymphal stage [77] and intensifies significantly when sexual maturity is reached. Because vitellogenesis requires high energy expenditure, the high level of expression of transcripts in the midgut glands with midgut may also indicate their influence on the regulation of energy mobilization from the energy storage compounds.…”

Section: Resultsmentioning

confidence: 99%

Expression profile of genes encoding allatoregulatory neuropeptides in females of the spider Parasteatoda tepidariorum (Araneae, Theridiidae)

et al. 2019

View full text Add to dashboard Cite

Allatoregulatory neuropeptides are multifunctional proteins that take part in the synthesis and secretion of juvenile hormones. In insects, allatostatins are inhibitors of juvenile hormone biosynthesis in the corpora allata while allatotropins, act as stimulators. By quantitative real-time PCR, we analyzed the gene expression of allatostatin A (PtASTA), allatostatin B (PtASTB), allatostatin C (PtASTC), allatotropin (PtAT) and their receptors (PtASTA-R, PtASTB-R, PtASTC-R, PtAT-R) in various tissues in different age groups of female spiders. In the presented manuscript, the presence of allatostatin A, allatostatin C, and allatotropin are reported in females of the spider P. tepidariorum. The obtained results indicated substantial differences in gene expression levels for allatoregulatory neuropeptides and their receptors in the different tissues. Additionally, the gene expression levels also varied depending on the female age. Strong expression was observed coinciding with sexual maturation in the neuroendocrine and nervous system, and to a lower extent in the digestive tissues and ovaries. Reverse trends were observed for the expression of genes encoding the receptors of these neuropeptides. In conclusion, our study is the first hint that the site of synthesis and secretion is fulfilled by similar structures as observed in other arthropods. In addition, the results of the analysis of spider physiology give evidence that the general functions like regulation of the juvenile hormone synthesis, regulation of the digestive tract and ovaries action, control of vitellogenesis process by the neuropeptides seem to be conserved among arthropods and are the milestone to future functional studies.

show abstract

“…Spiders also belong to JH‐dependent vitellogenesis. Recent results in spider Parasteatoda tepidariorum showed that fenoxycarb (a JH analog) could increase the transcript and protein levels of Vg (Bednarek et al ., 2019). However, many studies confirmed that JHs or JH analogs were not involved in the regulation of tick vitellogenesis because JHs or JH analog treatment had no effects on Vg synthesis or uptake in ticks (Sankhon et al ., 1999; Friesen & Kaufman, 2004; Thompson et al ., 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The VgR expression was also induced by 20E in Haemaphysalis longicornis (Boldbaatar et al ., 2008). In spider P. tepidariorum , Vg expression was upregulated by 20E (Bednarek et al ., 2019). In the present study, we also investigated the effects of JHs and ecdysteroids on vitellogenesis in the spider P. pseudoannulata , and our results showed that both VgRs and Vgs were upregulated by JHs and had no changes by ecdysteroids.…”

Section: Discussionmentioning

confidence: 99%

The importance of vitellogenin receptors in the oviposition of the pond wolf spider, Pardosa pseudoannulata

Yang

et al. 2021

Insect Science

View full text Add to dashboard Cite

Vitellogenin receptor (VgR) is crucial for vitellogenin (Vg) uptake by oocytes. VgR is less known in Arachnida, especially in spiders. Different from only one VgR in an arthropod species, two VgRs, VgR‐1 and VgR‐2, were found in the pond wolf spider, Pardosa pseudoannulata. Both VgRs had the typical domains of the low‐density lipoprotein receptor family except for the absence of the ligand‐binding domain 1 in VgR‐2. Spatiotemporal expression profiles showed that two VgR genes were consistently highly expressed in females and their ovaries, but VgR‐1 was 48‐fold that of VgR‐2 in ovaries. The transcriptional level of VgR‐1 was significantly downregulated by RNAi, but it did not work for VgR‐2 although several trials were performed. Vg‐1 and Vg‐2 might be the ligands of VgR‐1 because their expressions were also decreased in the dsVgR‐1‐treated females. Silencing VgR‐1 prolonged the pre‐oviposition period by 56 h. The expression of VgRs and Vgs were upregulated by juvenile hormones (JHs), which suggested that JHs were the essential factors to vitellogenesis in the spider. The present study revealed the importance of VgR‐1 in the spider oviposition, which will improve the understanding on VgR physiological functions in spiders.

show abstract

Vitellogenins in the spider Parasteatoda tepidariorum – expression profile and putative hormonal regulation of vitellogenesis

Cited by 11 publications

References 59 publications

Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider

Ovarian Transcriptomic Analyses in the Urban Human Health Pest, the Western Black Widow Spider

Expression profile of genes encoding allatoregulatory neuropeptides in females of the spider Parasteatoda tepidariorum (Araneae, Theridiidae)

The importance of vitellogenin receptors in the oviposition of the pond wolf spider, Pardosa pseudoannulata

Contact Info

Product

Resources

About