Vitis vinifera culture in a non-conventional bioreactor: the reciprocating plate bioreactor

Abstract

This paper is concerned with the potential use of a reciprocating plate bioreactor (RPB) for suspended plant cell cultures. The agitation mechanism of the RPB system, a plate stack, was ®rst evaluated in pure water and in pseudocells medium of 20, 40 and 60% of PCV. As the pseudocell concentration increases, the oxygen mass transfer coef®cient, K L a, signi®cantly decreases. Correlations were established for each plate stack and concentration with good prediction of K L a.Three fermentations were performed with Vitis vinifera cells, two in the RPB system and one in shake¯asks. Shakē ask cultures showed better performance whereas the ®rst fermentation performed with the RPB showed the lowest performance. The lower growth observed was attributed to the operating conditions for aeration and the dissolved oxygen control strategy. CO 2 stripping in the initial portion of the fermentation led to lower biomass growth. The second fermentation, with more appropriate operating conditions, appears to follow the trend of shake¯ask cultures but was terminated after 5 days due to contamination.The RPB has the potential to be used for suspended plant cell cultures but signi®cant research needs to be performed to ®nd optimal operating conditions but, more importantly, to make appropriate modi®cations to ensure the sterility of the bioreactor over long time periods.

Bioreactor technology for clonal propagation of plants and metabolite production

“…(B) Immobilized plant cell bioreactor (Choi et al, 1995). (C) Reciprocating plate bioreactor (Gagnon et al, 1999). (D) Flow bioreactors-(i) Single-column reactor, (ii) Radial flow reactor (Kino-oka et al, 1999).…”

“…8C), was used to investigate its potential for cell suspension culture. Vitis vinifera cells were used as a model culture in this bioreactor for their growth and viability (Gagnon et al, 1999). The bioreactor vessel was built with two concentric stainless steel tubes providing 17 L of working volume.…”

Anthocyanin Production from Plant Cell and Organ Cultures In Vitro

“…Numerous species, including Aralia cordata , Daucus carota , Nicotiana tabacum , Perilla frutescens , Vaccinium pahale , and Vitis vinifera ( Table 2 ), have been the subject of studies aimed at scale-up cell or callus suspension cultures, and a number of bioprocess parameters have been optimized [ 113 , 114 , 115 , 116 ]. Kobayashi et al [ 113 ] used Aralia cordata cell suspension cultures to evaluate a 500-L pilot-scale bioreactor culture for the synthesis of anthocyanins.…”