Vitreous levels of oxidative stress biomarkers and the radical-scavenger α1-microglobulin/A1M in human rhegmatogenous retinal detachment

Cederlund, Martin; Ghosh, Fredrik; Arnér, Karin; Andréasson, Sten; Åkerström, Bo

doi:10.1007/s00417-012-2113-6

Cited by 28 publications

(25 citation statements)

References 45 publications

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“…Up-regulated expression of A1M, both on mRNA-and protein levels, has been shown in human cell lines, primary keratinocytes, and skin and retinas explants after exposure to stress-conditions with hydrogen peroxide, hydroxyl radicals, Hb and heme [32,53,54]. This is supported by human in vivo data.…”

Section: Up-regulated A1m-expressionsupporting

confidence: 53%

A1M, an extravascular tissue cleaning and housekeeping protein

Åkerström¹,

Gram²

2014

Free Radical Biology and Medicine

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Section: Up-regulated A1m-expressionsupporting

confidence: 53%

A1M, an extravascular tissue cleaning and housekeeping protein

Åkerström¹,

Gram²

2014

Free Radical Biology and Medicine

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“…Cederlund et al. (2013) previously reported an elevated vitreous level of oxidative stress biomarkers in their small group of patients with rhegmatogenous retinal detachment (RRD), in addition to showing that oxidative stress was related to the RRD severity. Research has also shown that antioxidant treatments can reduce photoreceptor cell death in experimental retinal detachment in animal models (Rostein et al.…”

Section: Figurementioning

confidence: 98%

Characterization of the biological antioxidant potential in the vitreous fluid from patients with rhegmatogenous retinal detachment

Maeno

Suzuki

Adachi

et al. 2016

Acta Ophthalmologica

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“…One such molecule is the reductase, hemeand radical-binding protein A1M (α1-microglobulin), which was shown to have protective properties against oxidative stress in cell cultures, skin and placenta [16][17][18], and antioxidative therapeutic effects in vivo [19][20][21] (reviewed in [22;23]). A1M was also recently shown to be expressed and up-regulated in vitro in rat retinas [10].…”

Section: Introductionmentioning

confidence: 93%

“…For instance, oxidative stress has recently been shown to be involved in retinal degeneration during detachment in vivo, as well as in cell death observed in retinal explants in vitro [10]. Recent findings indicate that scavenging of reactive oxygen species (ROS) can attenuate retinal detachment-related photoreceptor death [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

The Role of Mitochondria, Oxidative Stress, and the Radical-binding Protein A1M in Cultured Porcine Retina

Åkerström

Cederlund

Bergwik

et al. 2017

Current Eye Research

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Purpose: The purpose of this study was to explore the relationship between oxidative stress, antioxidant defense, mitochondrial structure and biomechanical tissue support in the isolated porcine retina. Methods:Full-thickness retinal sheets were isolated from adult porcine eyes. Retinas were cultured for 2 or 48 hours using 1) a previously established low-support explant protocol with photoreceptors positioned against the culture membrane (porous polycarbonate) or 2) a high-support procedure developed by our group, apposing the Müller cell endfeet and inner limiting membrane (ILM) against the membrane. The grafts were analyzed by quantitative PCR, immunohistochemistry, and transmission electron microscopy (TEM), and culture medium was assayed for the cell damage and oxidative stress markers lactate dehydrogenase and protein carbonyls. Results:In explants cultured with physical support to the inner border, cone photoreceptors were preserved and lactate dehydrogenase levels were reduced, although an initial (2h), transient, increased oxidative stress was observed. Elevated expression of the antioxidants A1M (α1-microglobulin) and heme oxygenase-1 were seen in the mitochondria-rich inner segments after 48h compared to low-support counterparts.Housekeeping gene expression suggested a higher degree of structural integrity of mitochondria in high-support explants, and TEM of inner segments confirmed preservation of a normal mitochondrial morphology. Conclusion:Providing retinal explants with inner retinal support leads to mobilization of antioxidant proteins, preservation of mitochondrial function and increased cell viability.Consequently, the failure of low-support retinal cultures to mobilize an adequate response to 2 the oxidative environment may play an key role in their rapid demise. These findings shed new light on pathological reactions in biomechanically related conditions in vivo.

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Vitreous levels of oxidative stress biomarkers and the radical-scavenger α1-microglobulin/A1M in human rhegmatogenous retinal detachment

Cited by 28 publications

References 45 publications

A1M, an extravascular tissue cleaning and housekeeping protein

A1M, an extravascular tissue cleaning and housekeeping protein

Characterization of the biological antioxidant potential in the vitreous fluid from patients with rhegmatogenous retinal detachment

The Role of Mitochondria, Oxidative Stress, and the Radical-binding Protein A1M in Cultured Porcine Retina

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