Vitrification Affects Nuclear Maturation and Gene Expression of Immature Human Oocytes

Shahedi, Abbas; Hosseini, Ahmad; Khalili, Mohammad Ali; Yeganeh, Farshid

doi:10.29252/rmm.5.1.27

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…Moreover, the microRNA genes in this study have been reduced in the anti-apoptotic process, since the role of miRNAs in stimulating the expression of genes with pro-apoptotic or an-apoptotic behavior in order to produce anti-apoptotic effects have been recognized [60]. The antiapoptotic members of the Bcl-2 gene family have a vibrant role in regulating apoptosis, which promotes cell survival [105]. Apptosis is suppressed by anti-apoptotic genes, but increased expression of these genes causes cell death.…”

Section: Discussionmentioning

confidence: 93%

Use of Protein-Protein Interaction Network for Biomarker Identification in Oocyte Maturation

Farhadi

Fayazi

Roshanfekr

et al. 2019

RMM

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Background: Oocyte maturation begins at the embryonic stage and continues throughout life. The effect of Follicle- Stimulating hormone (FSH) on gene of genes was evaluated using GEO access codes for the data set GSE38345. Materials and Methods: The microarray data containing the gene expression information from cow oocytes show that their maturation is influenced by FSH. Data analysis was performed using GEO2R. After identifying the genes and examining the different genes expressed, two gene groups with increased and decreased expression were identified. The interaction of each of the gene groups was examined using the STRING database, based on the co-expression information. The meaningful sub networks were explored using the Clusterone software. Gene ontology was performed using the comparative GO database. The miRNA-mRNA interaction network was also studied based on the miRWalk database. Finally, meaningful networks and subnets obtained by the Cytoscape software, were designed. Results: Comparison of oocyte gene expression data between the pre-maturation and postmaturation stages after treatment with FSH revealed 5958 genes with increased expression and 4275 genes with decreased expression. Examination of the protein interaction network among the set of increased and decreased expression genes based on string information revealed 262 genes with increased expression and 147 genes with decreased expression (high confidence (0.7) data). RPS3, NUSAP1, TBL3, and ATP5H showed increased expression and were effective in the positive regulation of rRNA processing, cell division, mitochondrial ATP synthesis coupled proton, and in oxidative phosphorylation and progesterone-mediated functions. WDR46 and MRPL22 showed decreased expression, which were important in the regulation of SRP-dependent co-translational proteins targeting the membrane, RNA secondary structure, unwinding, and functional pathways of ribosomal and RNA polymerase. The most important miRNA genes in the protein network of increased and decreased gene expression were bta-miR-10b-5p and miR-29b-2-5p. Conclusion: Examination of the genes expressed in the oocyte maturation pathway revealed nuclear, mitochondrial, and miRNA genes. Increasing and decreasing gene expression helps maintain equilibrium, which can be a biological marker.

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Section: Discussionmentioning

confidence: 93%

Use of Protein-Protein Interaction Network for Biomarker Identification in Oocyte Maturation

Farhadi

Fayazi

Roshanfekr

et al. 2019

RMM

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“…Relative abundance of HSPA1A transcripts was significantly higher in vitrified-warmed oocytes and blastocysts, independent of LIF supplementation. Higher HSPA1A expression has already been observed after vitrification of human immature oocytes [ 52 ]. However, vitrification of canine oocytes [ 53 ] or prepubertal bovine oocytes does not modify HSPA1A -expression.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos

Vendrell-Flotats

García-Martínez

Martínez-Rodero

et al. 2020

IJMS

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Oocyte cryopreservation has a significant impact on subsequent embryonic development. Herein, we investigated whether supplementing in vitro maturation medium with Leukemia Inhibitory Factor (LIF) prior to vitrification affects embryo development and gene expression at different embryo developmental stages. A panel of genes including maternal effect, epigenetics, apoptosis and heat stress was relatively quantified. The results show reduced cleavage rates after vitrification, regardless of the LIF treatment. Although not statistically different from control-vitrified oocytes, oocyte apoptosis and the blastocyst yield of LIF-vitrified oocytes were similar to their non-vitrified counterparts. Vitrification increased oocyte ZAR1, NPM2 and DPPA3 gene expression while its expression decreased in LIF-vitrified oocytes to similar or close levels to those of non-vitrified oocytes. With a few gene-specific exceptions, vitrification significantly increased the expression of DNMT3A, HDAC1, KAT2A, BAX and BCL2L1 in oocytes and most stages of embryo development, while comparable expression patterns for these genes were observed between LIF-vitrified and non-vitrified groups. Vitrification increased HSPA1A expression in oocytes and HSP90AA1 in 2-cell embryos. Our data suggest that vitrification triggers stage-specific changes in gene expression throughout embryonic development. However, the inclusion of LIF in the IVM medium prior to vitrification stimulates blastocyst development and several other developmental parameters and induces oocytes and embryos to demonstrate gene expression patterns similar to those derived from non-vitrified oocytes.

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Comparing the effects of vitrification, before and after mouse oocyte in vitro maturation on developmental competence, changes in epigenetic regulators and stress oxidative response

Momeni,

Haghpanah,

Nematollahi-Mahani

et al. 2023

Biochemical and Biophysical Research Communications

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Vitrification Affects Nuclear Maturation and Gene Expression of Immature Human Oocytes

Cited by 5 publications

References 32 publications

Use of Protein-Protein Interaction Network for Biomarker Identification in Oocyte Maturation

Use of Protein-Protein Interaction Network for Biomarker Identification in Oocyte Maturation

In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos

Comparing the effects of vitrification, before and after mouse oocyte in vitro maturation on developmental competence, changes in epigenetic regulators and stress oxidative response

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