Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study

Mohsenzadeh, Mehdi; Tabibnejad, Nasim; Vatanparast, Mahboubeh; Anbari, Fatemeh; Khalili, Mohammad Ali; Karimi-Zarchi, Mojgan

doi:10.18502/ijrm.v17i3.4516

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…It is unclear if our enriched culture system could be the cause for the relatively low survival rates following vitrification. It has been postulated that IVM oocytes are more susceptible to vitrification, and several reports have described the negative effects of vitrification on oocyte survival when IVM is involved [ 33 , 34 ]. However, as data on survival rates after vitrification and warming from oocytes and embryos after IVM in oncological patients are scarce, evaluation on its efficacity is currently unfeasible.…”

Section: Discussionmentioning

confidence: 99%

Outcomes of Different In Vitro Maturation Procedures for Oocyte Cryopreservation for Fertility Preservation and yet Another Live Birth in a Cancer Patient

Nogueira

Fajau-Prevot²,

Clouet³

et al. 2023

Life

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To ensure patient care in an oncological fertility preservation (FP) programme, specialists must provide technology that best suits the patients’ clinical conditions. In vitro oocyte maturation (IVM) and ovarian tissue cryopreservation (OTC) are possible fertility preservation treatments for women in need of urgent oncological treatment. IVM consists of the retrieval of immature oocytes from small antral follicles, with no or minimal ovarian stimulation by gonadotropins. Therefore, IVM has become a pertinent option for fertility preservation, especially for cases whereby ovarian stimulation is unfeasible or contra-indicated. Existing data on immature oocytes, retrieved transvaginally (OPU-IVM) or extracted from ovarian tissue ‘ex vivo’ (OTO-IVM), are still limited on technical consistency, efficacy, and safety. The present retrospective cohort study includes 89 women undergoing fertility preservation using IVM methodologies and 26 women undergoing ovarian stimulation (OS) in concomitant period. In total, 533 immature oocytes were collected from IVM patients, achieving a maturation rate of 57% and 70% in OTO-IVM and 73% and 82% in OPU-IVM at 24 h and 48 h in culture, respectively. The observed high maturation rates might be due to the use of patients’ serum in its innate status, i.e., without heat-inactivation. This permitted 7.6 ± 5.7 and 4.6 ± 4.9 oocytes to be vitrified in OTO-IVM and OPU-IVM, respectively, compared to 6.8 ± 4.6 from OS patients. Regarding OS patients, two of them underwent embryo transfer following the insemination of warmed oocytes after complete remission, resulting in a single live birth from one patient. Upon follow-up of two OTO-IVM patients after the termination of their oncological treatment, a total of 11 warmed oocytes lead to a transfer of a single embryo, but pregnancy was not achieved. From OPU-IVM, six embryos were transferred in three patients 4.25 years after oocyte vitrification, leading to the live birth of a healthy boy. The present case of live birth is among the first the cases reported so far and supports the notion that IVM might be a relevant and safe FP option for cancer patients when oocyte preservation is required but ovarian stimulation is contra-indicated.

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Section: Discussionmentioning

confidence: 99%

Outcomes of Different In Vitro Maturation Procedures for Oocyte Cryopreservation for Fertility Preservation and yet Another Live Birth in a Cancer Patient

Nogueira

Fajau-Prevot²,

Clouet³

et al. 2023

Life

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“…Though successful birth after cryopreservation of germinal vesicle (GV)-stage oocytes with IVM has been reported in 1998 [ 139 ], concerns regarding higher rates of chromosomal abnormalities of immature oocytes after cryopreservation exist [ 140 ]. It has been found that vitrification of immature oocytes before IVM can adversely influence oocyte maturation, viability, and subcellular oocyte quality [ 87 ]. A meta-analysis based on 14 studies that assessed maturation rate after vitrification of metaphase I (MI)/ GV oocytes concluded that oocyte vitrification decreased the maturation rate by 24% [ 141 ].…”

Section: Cryopreservation and Other Physical Factorsmentioning

confidence: 99%

Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte

et al. 2021

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In vitro maturation (IVM) of oocytes is a promising assisted reproductive technology (ART) deemed as a simple and safe procedure. It is mainly used in patients with impaired oocyte maturation and in fertility preservation for women facing the risk of losing fertility. However, to date, it is still not widely used in clinical practice because of its underperformance. The influencing factors, such as biphasic IVM system, culture medium, and the supplementation, have a marked effect on the outcomes of oocyte IVM. However, the role of different culture media, supplements, and follicular priming regimens in oocyte IVM have yet to be fully clarified and deserve further investigation.

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“…As a result, unconventional ovarian stimulation protocols may be used, which have not yet been confirmed to be comparable with classical protocols. Often, the only option remains cryopreservation after in vitro oocyte maturation, which further adds unevaluated factors to the fertility potential of such oocytes [ 12 ].…”

mentioning

confidence: 99%

From glass to life: a commentary on the assessment of the reproductive potential of cryopreserved human oocytes

Plancha

Kovačič

2022

J Assist Reprod Genet

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Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study

Abstract: .

Cited by 6 publications

References 35 publications

Outcomes of Different In Vitro Maturation Procedures for Oocyte Cryopreservation for Fertility Preservation and yet Another Live Birth in a Cancer Patient

Outcomes of Different In Vitro Maturation Procedures for Oocyte Cryopreservation for Fertility Preservation and yet Another Live Birth in a Cancer Patient

Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte

From glass to life: a commentary on the assessment of the reproductive potential of cryopreserved human oocytes

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