Vitrification of human oocytes and different development stages of embryos: An overview

Vitrification of Day 2–3 Human Embryos Using Various Methods

“…Only standard laboratory devices, storage tanks, and training of the embryologists, which is quite easy for a standard IVF laboratory team, are necessary for this purpose. Therefore, any laboratory can convert to routine vitrifi cation within a few days without any specifi c setup [ 40 ].…”

Survival and developmental competence of bovine embryos at different developmental stages and separated blastomeres after vitrification in different solutions

“…Also, experiment 1 of the present study showed that, although the survival of the EG-vitrified blastocyst group was significantly lower than that of the group vitrified in the EG + PG combination, hatching ability did not significantly differ between the two groups. EG is more commonly used for the early stages of embryo vitrification procedures, and is also used in combination with other chemicals, such as dimethyl sulfoxide (Ghorbani et al 2012) and disaccharide molecules, which help draw out more water from the cells (Szell et al 1989;Mukaida et al 1998), and reduce the exposure time of CPA to toxins (Elnahas et al 2013). Moreover, the total number of cells in blastocysts vitrified with EG + PG was significantly higher than that of EG alone.…”

Assessment of 1,2-propanediol (PrOH) genotoxicity on mouse oocytes by comet assay

“…We decided to describe our preliminary results on PrOH assays, because PrOH is used in some protocols to vitrify oocytes (14,35) and embryos (36). However, most internationally published oocyte and embryo vitrification studies did not use PrOH but used EG and DMSO mixture (37)(38)(39). In light of our previous results, vitrification with DMSO and EG appears to us to be a safe method, even at high concentrations (16).…”