Vitrification of zona-free rabbit expanded or hatching blastocysts: a possible model for human blastocysts

Cervera, Rita P.; García-Ximénez, F.

doi:10.1093/humrep/deg428

Cited by 31 publications

(16 citation statements)

References 42 publications

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“…In vivo-produced mouse morulae that were cultured till the embryos hatched through the zona pellucida and then vitrified achieved a survival rate of 77% (79/103) after warming (Zhu et al 1996). Denuded (by acidic treatment) and then vitrified rabbit blastocysts resulted in 91% survival after warming (Cervera & Garcia-Ximenez 2003). When assisted hatching, using diode laser, was conducted before freezing of human blastocysts, 75.4% post-thaw survival and 31.4% clinical pregnancies were achieved (Kung et al 2003).…”

Section: Manipulations To the Zona Pellucidamentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

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Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and mice. Two basic cryopreservation techniques rule the field -controlled-rate freezing, the first to be developed, and vitrification, which, in recent years, has gained a foothold. While much progress has been achieved in human medicine, the cattle industry, and in laboratory animals, this is far from being the case for most other mammals and even less so for other vertebrates. The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.

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Section: Manipulations To the Zona Pellucidamentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

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“…In this way, the damage we observed in zona-free vitrified blastomeres might have been caused by osmotic stress that occurred during the addition and removal of concentrated cryoprotective solutions (11)(12)(13)(14) or during the removal of excess vitrification solution (VS) before cooling. The cryotop method involves the removal of the majority of the VS so that only a thin layer covers the biologic sample (7,8).…”

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confidence: 99%

Vitrification of isolated human blastomeres

Escribá

Grau

Escrich

et al. 2010

Fertility and Sterility

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“…These can be classified into two main categories: [1] hatching of the ZP performed by either laser, acidified Tyrode's solution, repeated micropipetting, or spontaneously by extending the culture of expanding blastocysts (12)(13)(14)(15)(16), and [2] blastocele collapse, passively by puncture or actively by microsuction (11,(17)(18)(19). Clinical trials adopting these techniques have shown significant improvement in terms of survival, fertilization, and pregnancy rates (15,(17)(18)(19).…”

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confidence: 99%