Objective
Determine the permeability of excised canine vocal fold lamina propria
Study Design
Basic Science
Methods
Vocal folds were excised from canine larynges and mounted within a device to measure the flow of 0.9% saline through the tissue over time. The resultant fluid volume displaced over time was then used in a variation of Darcy’s law to calculate the permeability of the tissue. Permeability was found through each anatomical plane of the vocal fold, with 5 samples per plane. Permeability was also found for lamina propria stretched to 10%, 20%, and 30% of initial length to determine the effects of tensile strain on permeability, with 5 samples per level of strain.
Results
Permeability was found to be 1.40×10−13 m3s/kg through the sagittal plane, 1.00×10−13 m3s/kg through the coronal plane, and 4.02×10−13 m3s/kg through the axial plane. It was significantly greater through the axial plane than both the sagittal (p=0.025) and coronal (p=0.009) planes. Permeability under strain through the sagittal plane was found to be 1.94×10−13 m3s/kg under 10% strain, 3.35×10−13 m3s/kg under 20% strain, and 4.80×10−13 m3s/kg under 30% strain. The permeability significantly increased after 20% strain (p<0.05).
Conclusion
Permeability in canine vocal fold lamina propria was found to be increased along the anterior-posterior axis, following the length of the vocal folds. This may influence fluid distribution within the lamina propria during and after vibration. Similarly, permeability increased after 20% strain was imposed on the lamina propria, and may influence vocal fold dynamics during certain phonation tasks.