Introduction and objective:In the period of transition from the reproductive to post-reproductive phase, clinical problems and changes of various character and intensity occur, which can affect the life of women. The aim of this quantitative study was to evaluate the factors affecting quality of life and quality of health of Czech women during the transition from reproductive to post-reproductive life. Methods: The data were obtained using two standardized questionnaires -(1) the WHO's WHOQOL-Bref and (2) Women's Health Questionnaire, (WHQ), as well as one non-standardized questionnaire to identify the women's quality of life and quality of health. Results: In relation to the quality of life, a statistical analysis showed that physical health was evaluated as the best item, while the home environment was considered to be the worst of the evaluated items. A comparison of the quality of health variables showed that during the climacteric, respondents were most troubled by vasomotor symptoms and least troubled by anxiety/fear. Most respondents perceived their quality of life as good, and the majority of women were satisfied with their health. Conclusions: A comparison of the quality of health variables showed that during the climacteric, respondents were most troubled by vasomotor symptoms and least troubled by anxiety/fear. Most respondents perceived their quality of life as good, and the majority of women were satisfied with their health.