Volatile compounds from lychee (Litchi
chinesis
Sonn.), a tropical fruit native to China, were
extracted
using both Freon 113 and ethyl acetate solvents. The odor-active
compounds present in the fruit
were isolated and characterized using gas chromatography/olfactory
(GC/O), chromatography, and
mass spectrometry. Authentic standards were used to determine mass
spectral, retention index,
and odor match. GC/O analysis detected at least 60 odor-active
volatiles in the fruit extract. More
odor-active volatiles were detected in the ethyl acetate extract than
in the nonpolar Freon extract.
Among the compounds that had significant odor activity, geraniol,
guaiacol, vanillin, 2-acetyl-2-thiazoline, 2-phenylethanol, unknown (58),
(Z)-2-nonenal, β-damascenone, 1-octen-3-ol,
Furaneol,
and linalool were found to be the most odor-active. On the basis
of their calculated odor activity
values (OAV), isobutyl acetate, guaiacol, cis-rose oxide,
2-acetyl-2-thiazoline, β-damascenone,
Furaneol, linalool, (E)-2-nonenal, geraniol, and isovaleric
acid were determined to significantly
contribute to the aroma of this fruit. GC/O analysis confirmed
that 2-phenylethanol was probably
responsible for the floral character and that the citrus-fruity aroma
is due to the presence of many
odor-active terpenes, particularly geraniol. Although
cis-rose oxide was only 30% as active in GC/O
as the most potent odor, its high OAV indicates its importance to the
character of lychee odor. An
unknown sesquiterpene-like compound (58), with a lychee-like
odor characteristic of the fresh fruit,
was identified by GC/O as being a highly odor potent compound.
Taken together, the aroma of
lychee was determined to be due to the interaction between compounds
with floral, nutty, citrus,
and fruity aromas.
Keywords: Aroma; GC/O; lychee; volatiles