Globally, food waste constitutes a significant fraction of municipal solid waste (MSW), representing up to 30-40% of the total MSW. In Canada, for instance, 35.6 million tonnes of MSW was generated in 2018, of which 30-35% was food waste. A large proportion of this food waste is disposed of in landfills, leading to considerable environmental and economic impacts such as greenhouse gas emissions, biodiversity loss, and high waste management costs. To address this challenge, the development of sustainable and cost-effective technologies that can transform food waste into valuable products has gained attention. Acidogenic fermentation is an emerging biotechnology that utilizes food waste as a substrate to produce high-value products such as short-chain fatty acids (SCFAs), offering a sustainable solution by combining waste management with resource recovery. However, acidogenic fermentation processes have been mostly studied at high temperatures of 35 to 55⁰C. These temperatures are suitable for geographical locations with warmer climates but may require significant energy input for heating in cooler climates like Canada. Therefore, this study aims to evaluate and optimize the acidogenic fermentation process for food waste at room temperature of 22-24⁰C by investigating the impact of inoculum type (anaerobic digested sludge (AD), waste activated sludge (WAS), cow dung slurry (CD)), inoculum pre-treatment (heat pre-treated and nonheated), and pH (7, 9, 12, and uncontrolled) on hydrolysis and SCFA production (acidogenesis).The first part of the study investigated the impact of inoculum type and heat pretreatment of inoculum on the hydrolysis and acidogenesis of food waste. The non-heated inoculums achieved higher hydrolysis, regardless of the inoculum types (AD, WAS, and CD). The AD non-heated achieved the highest hydrolysis yield of 399 gm cum SCOD/kg