Volatile organic compound analysis by ion molecule reaction mass spectrometry for Gram-positive bacteria differentiation

Dolch, M.; Hornuß, Cyrill; Klocke, C.; Praun, Siegfried; Villinger, J.; Denzer, W.; Schelling, Gustav; Schubert, Sören

doi:10.1007/s10096-012-1654-2

Cited by 41 publications

(32 citation statements)

References 25 publications

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“…Different bacterial strains show distinct patterns of VOCs in vitro and in animal models [6,7,8,9,10,11,12]. Therefore, exhaled breath analysis might be used to identify the causative pathogen in patients suspected of CAP/HAP [13].…”

Section: Introductionmentioning

confidence: 99%

Exhaled Breath Metabolomics for the Diagnosis of Pneumonia in Intubated and Mechanically-Ventilated Intensive Care Unit (ICU)-Patients

Oort

Bruin

Weda

et al. 2017

IJMS

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The diagnosis of hospital-acquired pneumonia remains challenging. We hypothesized that analysis of volatile organic compounds (VOCs) in exhaled breath could be used to diagnose pneumonia or the presence of pathogens in the respiratory tract in intubated and mechanically-ventilated intensive care unit patients. In this prospective, single-centre, cross-sectional cohort study breath from mechanically ventilated patients was analysed using gas chromatography-mass spectrometry. Potentially relevant VOCs were selected with a p-value < 0.05 and an area under the receiver operating characteristics curve (AUROC) above 0.7. These VOCs were used for principal component analysis and partial least square discriminant analysis (PLS-DA). AUROC was used as a measure of accuracy. Ninety-three patients were included in the study. Twelve of 145 identified VOCs were significantly altered in patients with pneumonia compared to controls. In colonized patients, 52 VOCs were significantly different. Partial least square discriminant analysis classified patients with modest accuracy (AUROC: 0.73 (95% confidence interval (CI): 0.57–0.88) after leave-one-out cross-validation). For determining the colonization status of patients, the model had an AUROC of 0.69 (95% CI: 0.57–0.82) after leave-one-out cross-validation. To conclude, exhaled breath analysis can be used to discriminate pneumonia from controls with a modest to good accuracy. Furthermore breath profiling could be used to predict the presence and absence of pathogens in the respiratory tract. These findings need to be validated externally.

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Section: Introductionmentioning

confidence: 99%

Exhaled Breath Metabolomics for the Diagnosis of Pneumonia in Intubated and Mechanically-Ventilated Intensive Care Unit (ICU)-Patients

Oort

Bruin

Weda

et al. 2017

IJMS

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“…; Dolch et al . ). The range and count rates of product ions detected are greater in TSB, a complex nutrient‐rich commercially available culture media, compared to a SWF containing peptone, NaCl and FBS.…”

Section: Discussionmentioning

confidence: 97%

In vitro discrimination of wound-associated bacteria by volatile compound profiling using selected ion flow tube-mass spectrometry

et al. 2017

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AimsTo determine if bacterial species responsible for clinically relevant wound infection produce specific volatile profiles that would allow their speciation. Methods and ResultsSelected-Ion Flow Tube-Mass Spectrometry in full mass scan mode was used to analyse headspace gases produced by wound-associated bacteria grown in vitro, so as to enable identification of bacterial volatile product ion profiles in the resulting mass spectra. Applying multivariate statistical analysis (hierarchical clustering and principal component analysis) to the resultant mass spectra enabled clear speciation. Moreover, bacterial volatile product ions could be detected from artificially contaminated wound dressing material, although the pattern of product ions detected was influenced by culture conditions. ConclusionsUsing selected product ions from the SIFT-MS mass spectra it is possible to discriminate wound-associated bacterial species grown under specific in vitro culture conditions. Significance and Impact of StudyThe results of this study have shown that wound associated bacteria can be discriminated using volatile analysis in vitro and that bacterial volatiles can be detected from wound dressing material. This indicates that volatile analysis of wounds or dressing material to identify infecting microbes has potential and warrants further study.

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“…Indeed, indole is known to be missing from the metabolic profile of E. aerogenes and as a result, a test for indole is often used to discriminate between E. coli and E. aerogenes in clinical laboratories. It has also been reported that VOC and polar metabolites can decrease during growth which could also be influencing the chemiresistor response [20,[37][38][39][40].…”

Section: Discussionmentioning

confidence: 98%

Detection of bacterial metabolites for the discrimination of bacteria utilizing gold nanoparticle chemiresistor sensors

Webster

Cooper

Chow

et al. 2015

Sensors and Actuators B: Chemical

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Volatile organic compound analysis by ion molecule reaction mass spectrometry for Gram-positive bacteria differentiation

Cited by 41 publications

References 25 publications

Exhaled Breath Metabolomics for the Diagnosis of Pneumonia in Intubated and Mechanically-Ventilated Intensive Care Unit (ICU)-Patients

Exhaled Breath Metabolomics for the Diagnosis of Pneumonia in Intubated and Mechanically-Ventilated Intensive Care Unit (ICU)-Patients

In vitro discrimination of wound-associated bacteria by volatile compound profiling using selected ion flow tube-mass spectrometry

Detection of bacterial metabolites for the discrimination of bacteria utilizing gold nanoparticle chemiresistor sensors

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