Volatile organic compounds as a diagnostic marker of late blight infected potato plants: A pilot study

Laothawornkitkul, Jullada; Jansen, R.M.C.; Smid, Hans M.; Bouwmeester, Harro J.; Müller, Josef K.; Bruggen, A.H.C. van

doi:10.1016/j.cropro.2010.03.003

Cited by 40 publications

(28 citation statements)

References 29 publications

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“…Ethyl acetate and 3-methyl-1-butanol were the derivatives of oxygenated monoterpene and were identified only in the headspace of P. cinnamomi-inoculated lupin seedling samples, which indicates that different substrates infected by P. cinnamomi resulted in different VOCs. Schnürer et al (36) found 2-ethyl-1-hexanol in significant amounts in the headspace of P. infestans-infected samples, and considered this compound as a useful marker volatile for this pathogen; while Laothawornkitkul et al (24) found three marker volatiles (5-ethyl-2(5H)-furanone, (E)-2-hexenal, and benzene-ethanol) that were used as chemical signals for P. infestans. In the present study, none of these P. infestans marker volatile compounds were found in P. cinnamomi-inoculated samples, except 2-ethyl-1-hexanol, which was found at a relatively low level in both the inoculated and control samples.…”

Section: Discussionmentioning

confidence: 98%

“…None of these compounds have been reported previously for P. cinnamomi or other Phytophthora species (24,36). Using GC-FID analysis, only two specific volatiles (4-ethyl-2-methoxy phenol and 4-ethyl phenol) were identified in the second sampling period.…”

Section: Discussionmentioning

confidence: 99%

“…de Lacy Costello et al (11) studied VOCs from P. infestans or Fusarium coeruleum inoculated potato tubers and found benzothiazole and 2-ethyl-1-hexanol as the most abundant VOCs produced by both pathogens. Laothawornkitkul et al (24) studied the VOCs of P. infestans infected potato plants and found three marker volatiles including: (E)-2-hexenal, 5-ethyl-2(5H)-furanone, and benzene-ethanol. To date, volatile metabolites have not been identified for P. cinnamomi and may be useful in detecting this oomycete.…”

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Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry for Analysis of VOCs Produced by Phytophthora cinnamomi

Qiu¹,

Trengove

et al. 2014

Plant Disease

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Qiu, R., Qu, D., Trengove, R., Agarwal, M., Hardy, G. E. St. J., and Ren, Y. 2014. Headspace solid-phase microextraction and gas chromatographymass spectrometry for analysis of VOCs produced by Phytophthora cinnamomi. Plant Dis. 98:1099-1105.Volatile organic compounds (VOCs) from Phytophthora cinnamomiinfected lupin seedlings were collected by headspace solid-phase microextraction (HS-SPME). The sampling was done 28 to 44, 52 to 68, and 76 to 92 h after inoculation (HAI). The HS-SPME samples were analyzed by gas chromatography-flame ionization detector (GC-FID) to assess the differences in volatile compounds between the P. cinnamomi-infected lupin seedlings and the control. Three specific peaks were identified after 52 to 68 h with the infected lupin seedlings, at which time there were no visible aboveground symptoms of infection. Subsequently, the VOCs of five different substrates (V8A, PDA, lupin seedlings, soil, and soil + lupin seedlings) infected with P. cinnamomi and the corresponding controls were analyzed by gas chromatography-mass spectrometry (GC/MS). A total of 87 VOCs were identified. Of these, the five most abundant that were unique to all five inoculated substrates included: 4-ethyl-2-methoxyphenol, 4-ethylphenol, butyrolactone, phenylethyl alcohol, and 3-hydroxy-2-butanone. Therefore, these metabolites can be used as markers for the identification of P. cinnamomi in different growing environments. Some VOCs were specific to a particular substrate; for example, 2,4,6-rrimethylheptanes, dl-6-methyl-5-hepten-2-ol, dimethyl trisulfide, 6,10-dimethyl-5,9-undecadien-2-ol, and 2-methoxy-4-vinylphenol were specific to P. cinnamomi + V8A; heptanes and 5-methyl-3-heptaneone were specific to P. cinnamomi + PDA; 3-methyl-1-butanol, ethyl acetate, 2-methyl-propanoic acid, ethyl ester, and ethyl ester 2-methyl-butanoic acid were specific to P. cinnamomi-inoculated lupin seedlings; and benzyl alcohol and 4-ethyl-1, 2-dimethoxybenzene were only detected in the headspace of inoculated soil + lupin seedlings. Results from this investigation have multiple impacts as the volatile organic profiles produced by the pathogen can be utilized as an early warning system to detect the pathogen from contaminated field soil samples. Data from this investigation have also illuminated potential metabolic pathways utilized by the oomycete during infection which may serve as potential targets for the development of specific control strategies.Phytophthora cinnamomi is a soilborne oomycete filamentous plant pathogen that causes destructive diseases in agricultural, horticultural, and forestry settings (7,43,45). The damage caused by P. cinnamomi is mainly below ground level and frequently not investigated in detail; it is also often neglected or confused with other fungi. Control of the pathogen is difficult, and currently there is no available method to eradicate P. cinnamomi without destroying plants in the process (12). Therefore, current management strategies are designed around hygiene and quarantine measures to stop the spread...

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry for Analysis of VOCs Produced by Phytophthora cinnamomi

Qiu¹,

Trengove

et al. 2014

Plant Disease

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“…During normal growth, most plants produce intrinsic volatile organic compounds (VOCs) released through leaves, flowers and fruits (Tholl et al, 2006). In recent years, the sensing of VOCs for monitoring and early detection of diseases in plants and produce has emerged as a promising tool (Cardoza et al, 2002;Toome et al, 2010;Laothawornkitkul et al, 2010;Jansen et al, 2011;Spadafora et al, 2016). Headspace sampling followed by Gas Chromatography-Mass Spectrometry (GC-MS), GC-Flame Ionization Detector (GC-FID), and electronic noses for disease detection in plants and agricultural produce are techniques that have been evaluated and reported by many researchers (Doughty et al, 1996;Arshak et al, 2004;Deng et al, 2004;Ebel et al, 2006;Blasioli et al, 2010;Copolovici and Niinemets, 2010;Konduru et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Rapid and non–destructive detection of Pectobacterium carotovorum causing soft rot in stored potatoes through volatile biomarkers sensing

et al. 2017

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Early disease detection plays a significant role in the pre-and post-production management of specialty crops, that often are stored for several months prior to consumption. Potato is one of the most important specialty crops of the United States (U.S.). However, soft rot in potatoes due to pathogenic infections during bulk storage, accounts for substantial losses to the industry. This study was aimed at assessing the applicability of an emerging technology, portable field asymmetric ion mobility spectrometry (FAIMS), towards early detection of soft rot in potatoes during bulk storage. The FAIMS senses mobility of ions pertinent to volatile organic compounds (VOCs) released from inoculated tubers. In this study, potato tubers, inoculated with Pectobacterium carotovorum causing soft rot, were analyzed using FAIMS over a 30-day period in storage. Sterile water inoculated tubers were considered as healthy controls. Results suggest that FAIMS can detect soft rot as early as two days after inoculation (DAI) by effectively capturing VOCs associated with rot progression. The activity of pathogen and associated VOCs release was maximum during the second week after inoculation. A principal component analysis showed a clear distinction between the healthy and P. carotovorum inoculated tubers. Pair-wise classification models, Quadratic discriminant analysis and Naïve Bayes with leave-one-out cross validation confirmed the validity of FAIMS response with accuracies between 83 and 100% for both healthy and P. carotovorum

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“…As a result of the evolutionary arms race between pathogen and host, plants are equipped with defense signaling pathways [ 1 , 2 ]. Induced response mechanisms after a pathogen attack as well as the effect of the infection itself change the plants’ transcriptome profile [ 3 ], phytohormone biosynthesis [ 4 , 5 ], and chemical profile [ 6 – 8 ]. Herbivores use visual and/or chemical cues to locate and accept host plants, and pathogen infection is known to modulate herbivores’ host preference behavior, performance, and population dynamics and structure [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Inoculation of Transgenic Resistant Potato by Phytophthora infestans Affects Host Plant Choice of a Generalist Moth

et al. 2015

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Pathogen attack and the plant’s response to this attack affect herbivore oviposition preference and larval performance. Introduction of major resistance genes against Phytophthora infestans (Rpi-genes), the cause of the devastating late blight disease, from wild Solanum species into potato changes the plant-pathogen interaction dynamics completely, but little is known about the effects on non-target organisms. Thus, we examined the effect of P. infestans itself and introduction of an Rpi-gene into the crop on host plant preference of the generalist insect herbivore, Spodoptera littoralis (Lepidoptera: Noctuidae). In two choice bioassays, S. littoralis preferred to oviposit on P. infestans-inoculated plants of both the susceptible potato (cv. Desiree) and an isogenic resistant clone (A01-22: cv. Desiree transformed with Rpi-blb1), when compared to uninoculated plants of the same genotype. Both cv. Desiree and clone A01-22 were equally preferred for oviposition by S. littoralis when uninoculated plants were used, while cv. Desiree received more eggs compared to the resistant clone when both were inoculated with the pathogen. No significant difference in larval and pupal weight was found between S. littoralis larvae reared on leaves of the susceptible potato plants inoculated or uninoculated with P. infestans. Thus, the herbivore’s host plant preference in this system was not directly associated with larval performance. The results indicate that the Rpi-blb1 based resistance in itself does not influence insect behavior, but that herbivore oviposition preference is affected by a change in the plant-microbe interaction.

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Volatile organic compounds as a diagnostic marker of late blight infected potato plants: A pilot study

Cited by 40 publications

References 29 publications

Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry for Analysis of VOCs Produced by Phytophthora cinnamomi

Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry for Analysis of VOCs Produced by Phytophthora cinnamomi

Rapid and non–destructive detection of Pectobacterium carotovorum causing soft rot in stored potatoes through volatile biomarkers sensing

Inoculation of Transgenic Resistant Potato by Phytophthora infestans Affects Host Plant Choice of a Generalist Moth

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