Volatile organic compounds emitted from endophytic fungus Trichoderma asperellum T1 mediate antifungal activity, defense response and promote plant growth in lettuce (Lactuca sativa)

Wonglom, Prisana; S, Itó; Sunpapao, Anurag

doi:10.1016/j.funeco.2019.100867

Cited by 137 publications

(104 citation statements)

References 49 publications

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“…Our results confirmed results from previous studies showing the presence of aliphatic alcohols e.g. 2-methyl-1-butanol and 2-ethyl-1-hexanol in T. asperellum emissions (Sim et al 2019;Wonglom et al 2019), as well as sesquiterpenes and monoterpenes (Nieto-Jacobo et al 2017;Sim et al 2019;Wonglom et al 2020), and aliphatic esters and carbonyls (Kottb et al 2015). Moreover, in volatile emissions of T. asperellum, we identified compounds previously detected in other Trichoderma species but not previous reported for T. asperellum including sesquiterpenes such as β-farnesene found in T. atroviride (Stoppacher et al 2010) and T. asperelloides (Gal-Hemed et al 2011), β-bisabolene found in T. atroviride (Stoppacher et al 2010;Polizzi et al 2011) and T. asperelloides (Gal-Hemed et al 2011), and β-elemene and germacrene D in T. virens (Nieto-Jacobo et al 2017).…”

Section: Vocs Produced By Trichodermasupporting

confidence: 92%

“…In recent years, VOC's emitted by different strains of T. asperellum, such as: T1 (Wonglom et al 2020), LF15 (Sim et al 2019), LU1370 (Nieto-Jacobo et al 2017 and IsmT5 (Kottb et al 2015), have been analyzed. These investigations revealed the emission of 4 (Kottb et al 2015) to maximum 22 compounds (Kottb et al 2015;Wonglom et al 2019), whereas we detected 49 volatiles emitted by T. asperellum IZR D-11.…”

Section: Vocs Produced By Trichodermamentioning

confidence: 99%

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Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

Oszako

Войтка²,

Stocki

et al. 2020

Eur J Plant Pathol

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The protective effects of Trichoderma asperellum IZR D-11 as a biocontrol agent against the powdery mildew Erysiphe alphitoides infecting leaves of Quercus robur were evaluated for the first time. A strain of Trichoderma had earlier been isolated in Belarus, and was identified in this study as T. asperellum by sequencing of three genomic markers: internal transcribed spacer, translation elongation factor 1 alpha and RNA polymerase II subunit 2, with over 99.2% identity to corresponding genomic sequences in GenBank. When applied once in the first year just after onset of powdery mildew disease, T asperellum IZR D-11 reduced disease progression and continued to reduce powdery mildew levels during the following three years. Photosynthetic activity as represented by chlorophyll fluorescence measured in oak seedlings was increased in treated plants, and greater assimilate production was also found. The use of this antagonistic fungus increased the total water content in oak leaves suggesting that T. asperellum IZR D-11 can serve as a preventive measure to reduce energy losses in the process of water transpiration. GC-MS analysis detected 49 volatile compounds in the headspace of pure cultures of T. asperellum. Sesquiterpenes represented mainly by daucene, dauca-4(11),8-diene and isodaucene were the largest group of compounds emitted. We speculate that these volatiles from T. asperellum IZR D-11 may be involved in induced resistance in the plant, but further research is needed. The above results suggest that T. asperellum strain IZR D-11 has potential as a biocontrol agent of oak powdery mildew in forest nurseries.

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Section: Vocs Produced By Trichodermasupporting

confidence: 92%

Section: Vocs Produced By Trichodermamentioning

confidence: 99%

Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

Oszako

Войтка²,

Stocki

et al. 2020

Eur J Plant Pathol

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“…Biological control is considered an alternative way to control postharvest fruit rot disease. It is well known that Trichoderma species have many helpful qualities, including antibiosis [ 4 , 5 , 6 , 7 ], competition for nutrients and space [ 8 ], mycoparasitism [ 9 ], and induction of systemic resistance [ 10 , 11 , 12 ] to control disease pathogens, as well as to promote plant growth ability [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

Intana

Kheawleng

Sunpapao

2021

JoF

Self Cite

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Postharvest fruit rot caused by Fusarium incarnatum is a destructive postharvest disease of muskmelon (Cucumis melo). Biocontrol by antagonistic microorganisms is considered an alternative to synthetic fungicide application. The aim of this study was to investigate the mechanisms of action involved in the biocontrol of postharvest fruit rot in muskmelons by Trichoderma species. Seven Trichoderma spp. isolates were selected for in vitro testing against F. incarnatum in potato dextrose agar (PDA) by dual culture assay. In other relevant works, Trichoderma asperellum T76-14 showed a significantly higher percentage of inhibition (81%) than other isolates. Through the sealed plate method, volatile organic compounds (VOCs) emitted from T. asperellum T76-14 proved effective at inhibiting the fungal growth of F. incarnatum by 62.5%. Solid-phase microextraction GC/MS analysis revealed several VOCs emitted from T. asperellum T76-14, whereas the dominant compound was tentatively identified as phenylethyl alcohol (PEA). We have tested commercial volatile (PEA) against in vitro growth of F. incarnatum; the result showed PEA at a concentration of 1.5 mg mL−1 suppressed fungal growth with 56% inhibition. Both VOCs and PEA caused abnormal changes in the fungal mycelia. In vivo testing showed that the lesion size of muskmelons exposed to VOCs from T. asperellum T76-14 was significantly smaller than that of the control. Muskmelons exposed to VOCs from T. asperellum T76-14 showed no fruit rot after incubation at seven days compared to fruit rot in the control. This study demonstrated the ability of T. asperellum T76-14 to produce volatile antifungal compounds, showing that it can be a major mechanism involved in and responsible for the successful inhibition of F. incarnatum and control of postharvest fruit rot in muskmelons.

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“…Banerjee, 2019). These molecules could have a potential role against fungal diseases due to their antimicrobial effects (Ezra et al, 2004;Chen et al, 2018;Wonglom et al, 2020) and/or ability to induce expression of defence genes in plants (Malmierca et al, 2012;Naznin et al, 2014;Pieterse et al, 2014). YCB36 showed the ability to reduce O. novo-ulmi growth when grown in confronted plates, probably through emission of volatile antibiotic molecules commonly attributed to terpenes, alcohols, and carboxylic acids (Zhang et al, 2014 novo-ulmi is able to grow in the presence of certain inhibitory substances such as gallic acid and thymol, which could reflect its greater ability to cope with plant defensive metabolites.…”

Section: Inhibition Mechanisms Of O Novo-ulmi By Endophyte Ycb36mentioning

confidence: 99%

Endophyte inoculation enhancesUlmus minorresistance to Dutch elm disease

Martínez‐Arias

Sobrino-Plata

Ormeño-Moncalvillo

et al. 2020

Preprint

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Certain fungal endophytes are known to improve plant resistance to biotic stresses in forest trees. In this study, three stem fungal endophytes belonging to classes Cystobasidiomycetes, Eurotiomycetes and Dothideomycetes were selected among 210 isolates for their potential as enhancers of Ulmus minor resistance to Ophiostoma novo-ulmi. We evaluated phenotypic traits of these endophytes that could be beneficial for O. novo-ulmi inhibition in the host plant. Under in vitro conditions, the Dothideomycetous isolate YCB36 strongly inhibited O. novo-ulmi growth, released antipathogen VOCs, chitinases and siderophores, and overlapped with the pathogen in nutrient utilization patterns. These functional traits could explain the 40% reduction in leaf wilting due to O. novo-ulmi in elm trees pre-inoculated with this endophyte. Ulmus minor trees responded to endophyte inoculation with increased stomatal conductance and higher amounts of flavonoids and total phenolic compounds in xylem tissues, suggesting induction of defence metabolism.

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Volatile organic compounds emitted from endophytic fungus Trichoderma asperellum T1 mediate antifungal activity, defense response and promote plant growth in lettuce (Lactuca sativa)

Cited by 137 publications

References 49 publications

Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

Endophyte inoculation enhancesUlmus minorresistance to Dutch elm disease

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