Voltage dependence of acetylcholine-activated membrane conductance in sympathetic ganglion neurons

Selyanko, A. A.; Derkach, V. A.; Kurennyi, D. É.

doi:10.1007/bf02141326

Cited by 6 publications

(14 citation statements)

References 8 publications

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“…The I-V relationship for the whole-cell ACh-elicited current in PC12 cells greatly resembles that found for rat chromaffin (5) and rat SCG (6) Johnson, Washington University School of Medicine). Cultures were fed three times a week and used 5-10 days after plating.…”

supporting

confidence: 55%

“…Nonlinear current-voltage (I-V) relationships have been described for excitatory synaptic currents in rabbit superior cervical ganglion (SCG) cells (1) and mouse submandibular ganglion cells (2). Inwardly rectifying ACh-elicited currents have been seen in rat sympathetic ganglion cells (3,4), rat and mouse parasympathetic ganglion cells (L. Fieber and D. Adams, personal communication; ref. 2), rat adrenal chromaffin cells (5), and rat SCG cells (6). The inward rectification ofthe ACh-elicited currents in these cells apparently results from different mechanisms.…”

mentioning

confidence: 99%

“…The voltage dependence of channel opening appears to be responsible for the rectification in rat sympathetic ganglion cells (4). For rat SCG cells, Selyanko et al (6) have concluded that the rectification can be completely explained by the voltage dependence of the channel open time determined from AChreceptor current relaxations after voltage jumps. The mechanism responsible for rectification ofthe ACh-elicited current in rat adrenal chromaffin cells has not been thoroughly studied, although Hirano et al.…”

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confidence: 99%

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Rectification of acetylcholine-elicited currents in PC12 pheochromocytoma cells.

Ifune

Steinbach

1990

Proc. Natl. Acad. Sci. U.S.A.

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The current-voltage (I-V) relationship for acetylcholine-elicited currents in the rat pheochromocytoma cell line PC12 is nonlinear. Two voltage-dependent processes that could account for the whole-cell current rectification were examined, receptor channel gating and single receptor channel permeation. We found that both factors are involved in the rectification of the whole-cell currents. The voltage dependence of channel gating determines the shape of the I-V curve at negative potentials. The single-channel I-V relationship is inwardly rectifying and largely responsible for the characteristic shape ofthe whole-cell I-V curve at positive potentials. Johnson, Washington University School of Medicine). Cultures were fed three times a week and used 5-10 days after plating.Physiological Recordings. Recordings were obtained using the List EPC-7 patch clamp. Records from whole-cell and outside-out patches (12) were acquired using the PCLAMP programs (Axon Instruments, Foster City, CA) and a 386 Zenith personal computer. Data were analyzed using BINFITS (C. Lingle, Washington University School of Medicine). Pipettes were pulled from KG33 borosilicate capillaries by using a P-80/PC micropipette puller (Sutter Instrument, San Rafael, CA) and coated with Sylgard 182 (Dow). After fire-polishing, pipette resistances were 2-5 MQ when the pipettes were filled with sodium isethionate intracellular solution (see below). For whole-cell recordings, the EPC-7 circuitry was used to cancel the capacity charging transients and to compensate for the series resistance, which ranged from 5 to 30 MQ. Series resistances were 70-80% compensated. Whole-cell and single-channel records were filtered at 2 kHz with an eight-pole low-pass Bessel filter. All experiments were conducted at room temperature (18-19'C) unless specified otherwise.Whole-cell I-V relationships were determined from peak currents elicited by ACh application to cells held at various potentials between -80 and +80 mV.Voltage-jump records were obtained by bracketing a series of jumps in the presence of ACh with jumps done in the absence of agonist. Voltage-jump records were leaksubtracted averages of 12-18 jumps. Current relaxations were fit with a single exponential or the sum of two exponential components by using the BINFITS program. Best fits were determined by visual inspection.Single-channel I-V relationships were determined using voltage ramps and voltage jumps. An AI2020 event detector (Axon Instruments, Foster City, CA) was used to trigger the computer to initiate the voltage ramp or jump when a single Abbreviations: ACh, acetylcholine; SCG, superior cervical ganglion;I-V, current-voltage. 4794The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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confidence: 55%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Rectification of acetylcholine-elicited currents in PC12 pheochromocytoma cells.

Ifune

Steinbach

1990

Proc. Natl. Acad. Sci. U.S.A.

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“…Opening relaxations recorded from rat sympathetic ganglion cells were similar to those described here, with time constants of approximately 10 ms at voltages between -50 and -70 mV (rr2 of Selyanko et al 1988;Colquhoun et al 1989;Mathie et al 1990), values similar to the time constant of synaptic current decay in rabbit sympathetic neurons (Derkach et al 1983). In rat submandibular ganglion cells synaptic current decay was described as the sum of two exponentials, with Tfast of 5-9 ms and TS10W of 27-45 ms (Rang, 1981;Yawo, 1989).…”

Section: Variations In Inward Rectification Of Nachr Currentsmentioning

confidence: 60%

“…Mechanisms postulated to underlie inward rectification of nAChRs have focused either on permeation properties, in particular block by intracellular Mg2+ or other cations (Hirano et al 1987;Neuhaus & Cachelin, 1990), or on intrinsic voltage dependence (Selyanko, Derkach & Kurennyi, 1988;Yawo, 1989), or on combinations of both (Ifune & Steinbach, 1990b;Mathie, Colquhoun & Cull-Candy, 1990). The studies of Ifune & Steinbach (1990b) and Mathie et al (1990) Ifune & Steinbach (1990b) observed MgF+ block of single nAChR channels and its relief under Mg2+-free conditions, and voltage-dependent opening and closing relaxations in whole-cell recordings from PC12 cells.…”

Section: Inward Rectificationmentioning

confidence: 99%

Neuronal nicotinic acetylcholine receptor currents in phaeochromocytoma (PC12) cells: dual mechanisms of rectification.

Sands

Barish

1992

The Journal of Physiology

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SUMMARY1. Neuronal nicotinic acetylcholine receptor (nAChR) currents in PC12 cells were studied using single-channel and whole-cell gigaohm seal voltage-clamp techniques. Nicotinic AChR agonists were applied using external pipettes.2. The single-channel conductance of neuronal nAChRs in outside-out patches under Mg2+-free ionic conditions was 48 pS. In the absence of internal Mg2+ singlechannel currents (outside-out patches) did not rectify.3. Whole-cell nAChR currents recorded with normal internal solution (lacking Mg2+ chelators as described below) were strongly inwardly rectifying. Current vs.voltage relations showed a sharp inflexion near 0 mV, and outward current was never observed.4. Extensive dialysis with internal solution containing EDTA and Na2-ATP, chelators of intracellular Mg2+ (Mg2+), relieved rectification of instantaneous nAChR currents during voltage jumps from negative potentials. The instantaneous I-V relation became linear with voltage, in agreement with the expectation from singlechannel measurements made under similar ionic conditions. 5. Agonist-induced currents recorded under Mg2+-free conditions relaxed towards zero current during depolarizing voltage steps. Rectification of ACh-induced currents at the steady state could be described by a Boltzmann relation, with one-half of channels available at + 3.4 mV and an effective gating charge of -0 97. Channel availability was approximately 95% at the resting potential. Opening and closing relaxations under Mg2+-free conditions could be fitted by single exponential functions whose time constants were weakly voltage dependent. 6. A model of rectification was constructed incorporating two voltage-dependent processes: block by Mg?+ and intrinsic channel gating. The I-V relations predicted by this model for both normal and Mgd+-free conditions were in good agreement with the experimental data. We suggest that rectification of nAChR currents in these cells is due to concurrent activity of these two voltage-dependent processes. The relative contributions of these two mechanisms are frequency dependent, with block by Mg2i

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Mechanism of long-lasting block of ganglion nicotinic receptors by mono-ammonium compounds with long aliphatic chain

Kurenny

Selyanko

Derkach

et al. 1994

Journal of the Autonomic Nervous System

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Voltage dependence of acetylcholine-activated membrane conductance in sympathetic ganglion neurons

Cited by 6 publications

References 8 publications

Rectification of acetylcholine-elicited currents in PC12 pheochromocytoma cells.

Rectification of acetylcholine-elicited currents in PC12 pheochromocytoma cells.

Neuronal nicotinic acetylcholine receptor currents in phaeochromocytoma (PC12) cells: dual mechanisms of rectification.

Mechanism of long-lasting block of ganglion nicotinic receptors by mono-ammonium compounds with long aliphatic chain

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