Voltage-Dependent Anion Channel 2 of Arabidopsis thaliana (AtVDAC2) Is Involved in ABA-Mediated Early Seedling Development

Yan, Jinping; He, Hailong; Tong, Shibo; Zhang, Wanrong; Wang, Jianmei; Li, Xufeng; Yang, Yi

doi:10.3390/ijms10062476

Cited by 25 publications

(21 citation statements)

References 31 publications

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“…These included mitochondrial creatinekinase, microtubule-associated protein 2, mitochondrial heat shock protein 70 and several glycolytic enzymes, such as hexokinase (Hxk), aldolase, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). In Arabidopsis plants, Hexokinase 1 (HXK1) is an interaction protein of VDAC, and AtHXK1 overexpressing transgenic plants have faster germination kinetics, which coincides with observations on seed germination of AtVDAC2 antisense transgenic lines in the presence of ABA [22,30]. To gain insights into how VDAC1 affects plant response to cold stress, we performed a yeast two-hybrid screen using VDAC1 as bait.…”

Section: Discussionsupporting

confidence: 61%

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The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1

et al. 2013

IJMS

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The voltage-dependent anion channel (VDAC), a highly conserved major mitochondrial outer membrane protein, plays crucial roles in energy metabolism and metabolite transport. However, knowledge about the roles of the VDAC family in plants is limited. In this study, we investigated the expression pattern of VDAC1 in Arabidopsis and found that cold stress promoted the accumulation of VDAC1 transcripts in imbibed seeds and mature plants. Overexpression of VDAC1 reduced tolerance to cold stress in Arabidopsis. Phenotype analysis of VDAC1 T-DNA insertion mutant plants indicated that a vdac1 mutant line had faster germination kinetics under cold treatment and showed enhanced tolerance to freezing. The yeast two-hybrid system revealed that VDAC1 interacts with CBL1, a calcium sensor in plants. Like the vdac1, a cbl1 mutant also exhibited a higher seed germination rate. We conclude that both VDAC1 and CBL1 regulate cold stress responses during seed germination and plant development.

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Section: Discussionsupporting

confidence: 61%

“…VDAC1 , VDAC2 and VDAC4 , may play important roles in plant growth [20,21]. Transgenic Arabidopsis plants suppressing AtVDAC2 expression showed an abscisic acid (ABA)-insensitive phenotype, suggesting the role of VDAC in ABA signaling [22]. Transgenic rice plants overexpressing millet VDAC became tolerant to salinity stress [19].…”

Section: Introductionmentioning

confidence: 99%

The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1

et al. 2013

IJMS

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“…Proteins that were identified as CK responsive that are apparently involved in ABA–CK cross-talk include several well-known ABA response proteins, for example magnesium-chelatase subunit chlI (At4g18480; increased in response to ipt activation) and RNA-binding protein At2g21660 (decreased in response to HvCKX2 activation; mutant sensitive to ABA). They also included two proteins likely to be involved in ABA signalling (Yan et al , 2009): At3g26450 (decreased in response to ipt activation), which contains a ligand-binding domain similar to PYR/PYL/RCAR, and mitochondrial porin (At5g67500; increased in response to ipt activation).…”

Section: Discussionmentioning

confidence: 99%

Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation

Černý

Kuklová

Hoehenwarter

et al. 2013

Journal of Experimental Botany

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In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying the regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) and agrobacterial isopentenyl transferase (CaMV35S>GR>ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified >1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development, and/or hormone and light signalling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates, and organic acids. Comparison of the data sets obtained from activated CaMV35S>GR>HvCKX2 and CaMV35S>GR>ipt plants revealed unexpectedly extensive overlaps. Integration of the proteomic and metabolomic data sets revealed: (i) novel components of molecular circuits involved in CK action (e.g. ribosomal proteins); (ii) previously unrecognized links to redox regulation and stress hormone signalling networks; and (iii) CK content markers. The striking overlaps in profiles observed in CK-deficient and CK-overproducing seedlings might explain surprising previously reported similarities between plants with down- and up-regulated CK levels.

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“…However, the view of free and uncontrolled diffusion across the outer membrane has been challenged with transport through porins (voltage-dependent anion channel/mitochondrial porins [VDACs]) likely to be regulated. The Arabidopsis genome contains four VDAC genes, and their mutants show differential phenotypes, suggesting specific functions of the different VDAC proteins (113,216,238). Additionally, the intermembrane space (IMS) has been reported to maintain its own physiological microenvironment with respect to pH and glutathione redox potential (82,169), which requires at least limitation of diffusion.…”

Section: Passing the Outer Membranementioning

confidence: 99%

Mitochondrial Energy and Redox Signaling in Plants

Schwarzländer

Finkemeier

2013

Antioxidants & Redox Signaling

155

110

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Significance: For a plant to grow and develop, energy and appropriate building blocks are a fundamental requirement. Mitochondrial respiration is a vital source for both. The delicate redox processes that make up respiration are affected by the plant's changing environment. Therefore, mitochondrial regulation is critically important to maintain cellular homeostasis. This involves sensing signals from changes in mitochondrial physiology, transducing this information, and mounting tailored responses, by either adjusting mitochondrial and cellular functions directly or reprogramming gene expression. Recent Advances: Retrograde (RTG) signaling, by which mitochondrial signals control nuclear gene expression, has been a field of very active research in recent years. Nevertheless, no mitochondrial RTG-signaling pathway is yet understood in plants. This review summarizes recent advances toward elucidating redox processes and other bioenergetic factors as a part of RTG signaling of plant mitochondria. Critical Issues: Novel insights into mitochondrial physiology and redoxregulation provide a framework of upstream signaling. On the other end, downstream responses to modified mitochondrial function have become available, including transcriptomic data and mitochondrial phenotypes, revealing processes in the plant that are under mitochondrial control. Future Directions: Drawing parallels to chloroplast signaling and mitochondrial signaling in animal systems allows to bridge gaps in the current understanding and to deduce promising directions for future research. It is proposed that targeted usage of new technical approaches, such as quantitative in vivo imaging, will provide novel leverage to the dissection of plant mitochondrial signaling.

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Voltage-Dependent Anion Channel 2 of Arabidopsis thaliana (AtVDAC2) Is Involved in ABA-Mediated Early Seedling Development

Cited by 25 publications

References 31 publications

The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1

The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1

Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation

Mitochondrial Energy and Redox Signaling in Plants

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