Voltage‐dependent calcium and chloride currents in S17 bone marrow stromal cell line

Abstract

The bone marrow stromal cell line S17 has been used to study hematopoiesis in vitro. In this study, we demonstrate the presence of calcium and chloride currents in cultured S17 cells. Calcium currents were of low amplitude or barely detectable (50-100 pA). Hence to amplify the currents, we have used barium as a charge carrier. Barium currents were identified based on their distinct voltage-dependence, and sensitivity to dihydropyridines. S17 cells also exhibited a slowly activating outward current without inactivation, most commonly seen when the sodium of the extracellular solution was replaced either by TEA (TEA/Cs saline) or NMDG (NMDG saline), or by addition of amiloride to the extracellular solution. This current was abolished either by 500 microM SITS (4,4'-diisothiocyanatostilbene-2-2'-disulfonic acid) or 500 microM DPC (diphenylamine-2-carboxylic acid) a cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel blocker, identifying it as a Cl(-) current. RT-PCR identified the presence of ENaC and CFTR transcripts. CFTR blockade reduced cell proliferation, suggesting that this channel plays a physiological role in regulation of S17 cell proliferation.

In vitro Antitumoral Activity of Compounds Isolated from Artemisia gorgonum Webb

“…Cell viability was compared with that of a murine nontumoral cell line (bone marrow stromal, S17 cells). Although S17 is not a primary cell culture, it is derived from nontumoral tissues and retains most of the functions of bone marrow stromal cells in vivo (Silva et al ., ). Thus, it was used in this study as a suitable model to measure selectivity of the compound towards tumoral cells.…”

Expression of potassium channels is relevant for cell survival and migration in a murine bone marrow stromal cell line

“…These last two channels are known to be involved directly with secretory mechanisms in other cell types. In addition, we have shown that the inhibition of CFTR, by diphenylamine‐2‐carboxylate decreased S17 cell proliferation (Silva et al, ).…”

“…So far, the diversity of S17 cell membrane ion channels and their function has not been fully elucidated, despite its role in hematopoiesis support. Our group has previously shown the functional presence and physiological contribution of three channels in S17 cells: an epithelial sodium channel (ENaC), a high-voltage-activated calcium channel (L-type calcium channel), and a cystic fibrosis transmembrane conductance regulator (CFTR; Silva et al, 2010). These last two channels are known to be involved directly with secretory mechanisms in other cell types.…”