Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode

Abstract

The working principle of a genosensor is based on the mechanism of ion‐channel mimetic sensors. The analytical signals generated upon hybridization processes were recorded by a redox active marker [Fe(CN)6]3−/4− present in the sample solution using voltammetric techniques. The developed genosensor was suitable for determination of 20‐mer complementary oligonucleotide sequence, and also of the PCR products containing the complementary 20‐mer sequence in various positions, with detection limits in the 10 pM range. The noncomplementary 20‐mer oligonucleotide sequence as well as the PCR product without complementary region generated very weak response. The good discrimination of the position of the complementary part in the PCR products was observed.

Electrochemical genosensor based on disc and screen printed gold electrodes for detection of specific DNA and RNA sequences derived from Avian Influenza Virus H5N1

“…This system was selective. The decrease of reduction/oxidation peak current of 15.1 ± 2.86 % recorded in presence of the fully complementary c-NC3 and 0.67 ± 1.65 % in presence of the non-complementary nc-NC3 target were observed [26]. These data indicated that measuring system based on SPGEs in the comparison to GDEs is more sensitive.…”

“…The selectivity of the genosensor confirms the lack of response in the case of nc-NC3 sequence. The detection of 20-mer DNA target derived from AIV H5N1 (c-NC3) with using classic gold electrodes modified with SH-NC3/MCH has been already reported [26]. This system was selective.…”

“…After selfassembling the mixed SH-NC3/MCH SAM, the electrode was treated with 1 mM solution of MCH to avoid non-specific adsorption and eliminate direct contact between the redox marker and the gold electrode surface. This step of genosensor preparation using SH-NC3 probe is widely applied [24][25][26][27][28][29][30]. The immobilization of SH-NC3/MCH SAM was confirmed by CV and OSWV in the presence of Fe(CN) 6 3-/4-as a redox marker.…”

“…The working principle of the biosensor proposed is based on the ion-channel mechanism originally developed by Umezawa [31] and precisely described in our previous work [26].…”

Electrochemical Sensors for Detections of Influenza Viruses: Fundamentals and Applications

“…Recently, in our laboratory, we have developed two genosensors working according to this mechanism based on the modiied gold electrode intended for the detection of speciic DN" sequence of avian inluenza virus "IV H5N1 using NH 2 -ssDN" or HS-ssDN" probes for the modiication of gold electrode [11,12].…”