Voltammetric Detection of Hg2+ Using Peptide-Functionalized Polymer Brushes

Abstract

Polymer brushes grafted by surface-initiated atom transfer radical polymerization (SI-ATRP) from the surface of Ir-based microelectrode arrays are explored as a platform for the fabrication of sensory coatings for the voltammetric detection of Hg 2þ . The polymer brush coatings are post-modified with a metallothionein derived peptide to enable the selective detection of Hg 2þ . The performance of the polymer brush modified microelectrode arrays was evaluated using both cyclic voltammetry (CV) as well as square-wave anodic stripping voltammetry (SWASV) experiments. These studies revealed that the polymer brush based coatings allowed the selective detection of Hg 2þ with detection limits in the subnanomolar range.

A highly selective colorimetric fluorescent probe for detection of Hg2+ and its application on test strips

“…Pyrazole (1.36 g, 20 mmol), p-uorobenzaldehyde (2.48 g, 20 mmol) and anhydrous potassium carbonate (6.90 g, 50 mmol) were dissolved in 50 mL of DMF, the mixture was allowed to warm 110 C and stirred at this ambient temperature for 24 h. Aer TLC showed the conversion of the starting material, the mixture was cooled to room temperature, ltered. 22 The ltrate was diluted with 400 mL of distilled water and the mixture was extracted with 100 mL of acetic ether, the organic layer was dried with anhydrous sodium sulfate and then evaporated, the resultant residue was puried by a column chromatography (petroleum/ ethyl acetate, 7/1) to give compound 1 was a white powder (3.34 g, yield 91%); mp 82-84 C; 1 excess of hydrazine hydrate (0.95 g, 20 mmol), the mixture was heated to 80 C and reuxed for 12 h to give a dark red clear transparent solution. Then, the mixture was subjected to decompression and evaporation till a few solid was formed.…”

“…Aer which, the mixture was poured into 200 mL of deionized water, rested for 3 hours, and ltered. 23 The solid was washed alternately with distilled water and ethanol several times, and dried in vacuo to afford compound 3 as a pink solid (7.88 g, yield 70%); 1 ) and compound 1 (4.56 g, 10 mmol) were dissolved in 40 mL of ethanol, the mixture was heated to 80 C and reuxed for 2 h aer a catalytic amount of acetic acid (3.46 mL, 10 mmol) was added. Then the mixture was cooled to room temperature to yield a rose pink precipitate.…”

“…Then the mixture was cooled to room temperature to yield a rose pink precipitate. The mixture was ltered, the solid was washed with ethanol for several times and dried in vacuo to give sensor Pyr-Rhy (4.98 g, yield 79.3%); 1…”

“…Mercury (Hg), one of the most toxic pollutants, 1 is widely distributed in the environment and exists in the state of mercury metal, inorganic mercury and organic mercury compounds. 2,3 The organic mercury is the most harmful of the mercury-containing compounds, because it is hardly biodegradable and can be biomagnied in the food chain.…”

Environmental Sensing of Aquatic Systems at the University of Geneva

“…for detection of respectively Cu(ii), Pb(ii), Cd(ii), Zn(ii), Mn(ii), Fe(ii) and As(iii)). Electrochemically plated AuNP can be chemically functionalized with (bio-)polymers (d) [24] (e.g. for selective recognition and chemical preconcentration of target analytes prior their voltammetric detection).…”