Voltammetric Iodometric Titration of Ascorbic Acid with Dead-Stop End-Point Detection in Fresh Vegetables and Fruit Samples

Abstract

The present work describes a method for determining ascorbic acid, which combines iodometry with a voltammetric technique to detect the end point of the titration. In addition, the validity of the method applied to natural vegetable or fruit samples was assessed. The results were compared with those obtained by an accurate method such as HPLC using UV detection. Similar values of ascorbic acid for different natural samples were obtained by means of this approach (p > 0.05). The limit of quantification was 0.1 mg. This technique presents the advantage of other electroanalytical methods such as avoiding filtration or ultracentrifugation steps, with the additional benefit of using the platinum electrodes, which are routinely used in the laboratory. These facts allow a rapid and efficient quantification of ascorbic acid with very low cost of reagents and equipment.

Variation in Ascorbic Acid and Oxalate Levels in the Fruit of Actinidia chinensis Tissues and Genotypes

“…Relatively high concentrations of ascorbic acid are also reported in fruit of A. arguta , A. chinensis ( , ), A. chrysantha , and A. polygam , with very high levels in A. eriantha , A. latifolia (>1% FW) (), and A. kolomikta (). Ascorbic acid is determined according to a variety of established methods including colorimetric titration (), enzymatic assays based on ascorbate peroxidase or oxidase ( , ), electrochemical methods ( − ), and HPLC coupled with electrochemical detection ( , ) or by HPLC coupled with UV detection ( , ).…”

Determination of Vitamin C in Foods Using the Iodine-Turbidimetric Method Combined with an Infrared Camera

“…However, the common plants generally contain little or no heavy metal ions, and thus do not affect the measurement. In addition, in the study combining the voltammetric technique with iodometry (of which the main chemical process was also the redox between I 2 and VC), Roxana A. Verdini et al treated natural samples with ascorbate oxidase and carried out iodometric titration [26].…”

Comparison of Pt and Ni foil electrodes for amperometric sensing of ascorbic acid

“…Rapid, sensitive real time detection of H 2 A concentration is of great interest. Many analytical methods have been developed for determination of H 2 A, including titrimetry [1], enzymatic methods [2,3], spectrophotometry [4,5], chemiluminescence [6], electroanalysis [7][8][9][10][11][12] and chromatography [14,15]. Among these methods, electroanalysis is noteworthy because it offers simplicity, convenience, and sensitivity.…”