Volumetric oxygen transfer coefficients (kLa) in batch cultivations involving non-Newtonian broths

Badino, Alberto Colli; Facciotti, Maria Cândida Reginato; Schmidell, Willibaldo

doi:10.1016/s1369-703x(01)00092-4

Cited by 97 publications

(55 citation statements)

References 21 publications

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“…oxygen uptake rate (OUR), oxygen transfer rate (OTR), volumetric mass transfer coefficient (K L a), enhancement factor (E), oxygen utilization rate (OD), Damköhler number (Da), and effectiveness factor (η) during Phase IV were determined by a Dynamic Method for all runs since it is useful when the dissolved oxygen concentration is above the critical value (C crit ) as in this study. 22 These parameters are tabulated in Table 3.…”

Section: Oxygen Transfer Characteristics Of the Bioprocessesmentioning

confidence: 99%

Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed‐batch operation

Çalı́k

İnankur

Soyaslan

et al. 2009

J of Chemical Tech & Biotech

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Section: Oxygen Transfer Characteristics Of the Bioprocessesmentioning

confidence: 99%

Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed‐batch operation

Çalı́k

İnankur

Soyaslan

et al. 2009

J of Chemical Tech & Biotech

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“…Badino et al (2001) in his review stated that several authors employing the correlation by incorporating additional variables such as apparent viscosity (μ ap ), surface tension (σ) and dimensionless numbers for their respective bioprocesses. However, if the intention was to use k L a for design and scale up of a bioreactor, it was necessary to generate a precise correlation incorporating physical properties such as viscosity, surface tension, density and dimensionless numbers.…”

Section: Discussionmentioning

confidence: 99%

“…In the aerobic fungal fermentations, sufficient supply of oxygen to the microorganisms is crucial. Systematic engineering approaches to tackle this problem have been made by many researchers (Arjunwadkar et al 1998;Badino et al 2001). The oxygen mass transfer rate in the biocalorimeter depends on variables, such as the physical properties of the liquid (viscosity, surface tension, etc.…”

Section: Volumetric Mass Transfer Coefficient Correlation Studiesmentioning

confidence: 99%

“…13. Then several researchers extended the same relationship (Yagi and Yoshida 1975;Badino et al 2001) to other bioprocess systems.…”

Section: Volumetric Mass Transfer Coefficient Correlation Studiesmentioning

confidence: 99%

“…For large-scale systems, power consumption during aeration and agitation was a vital factor in deciding the operating cost. The BioRTCal results on the impact of broth viscosity (μ ap ) on protease production were further used to obtain a correlation as proposed by Cooper et al (1944) and Badino et al (2001).…”

Section: Volumetric Mass Transfer Coefficient (K L A) For Biortcal Scmentioning

confidence: 99%

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Impact of aeration and agitation on metabolic heat and protease secretion of Aspergillus tamarii in a real-time biological reaction calorimeter

Dhandapani

Surianarayanan

Dhilipkumar

et al. 2012

Appl Microbiol Biotechnol

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The effects of aeration and agitation on metabolic heat, alkaline protease production and morphology for Aspergillus tamarii MTCC5152 are reported in this manuscript. Measurement of metabolic heat has been attempted by the continuous and dynamic heat balance method in a biological real-time reaction calorimeter. At lower agitation intensities, growth-related processes were dominating. As a result the protease activity and the product heat yields were lower than those for 350 and 450 rpm. Although biomass growth was necessary to obtain maximum protease yield, agitation seemed to play a vital role in the protease production process. Energy dissipation per circulation function of the process is also deduced from power input. At optimal conditions, 350 rpm and 1 vvm, the gassed power required was 0.133 W. Pellet morphology and protease production were studied under different aeration and agitation intensities of A. tamarii. Pellet structure was considerably influenced by DO, a higher DO level resulted in denser pellets (1,018.4 kg/m(3)) leading to higher protease activity. Coupling of hydrodynamics and bio-reaction highlighted the complex relationship between energy dissipation, substrate uptake rate and fungal physiology. This study emphasised the potential of biocalorimetry as a reliable monitoring and robust control tool for aerobic fermentation of A. tamarii, using agricultural by-products.

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Oxygen Transfer Rate Determination: Chemical, Physical and Biological Methods

Garcı́a-Ochoa

Gómez

2010

Encyclopedia of Industrial Biotechnology

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A proper mechanical design of the bioreactor is aimed at maintaining the environmental and nutritional conditions for optimal growth and/or product formation. Among the various environmental and nutritional factors that influence the aerobic bioprocess, oxygen is a critical parameter since it is the only nutrient that has to be provided continuously, even in batch cultures. The oxygen transfer rate (OTR) determination is essential in order to establish aeration efficiency and to quantify the effects of the operation variables on the requirement of oxygen since dissolved oxygen concentration is dependent on transport processes. Under these conditions OTR (and K L a ) is one of the key parameters involved in the design, operation, and scale‐up of bioreactors There are several approaches to determine OTR. The most common measuring methods applied can be classified depending on whether the determination is realized in the absence of microorganisms or with dead cells (chemical and physical methods), or in the presence of biomass that consumes oxygen at the same time that the measurement is carried out (biological methods). Sometimes when OTR from a physical method is determined, it must take into account the enhancement factor, E . In recent literature predictive methods have been proposed to calculate k L a and E , and thus find the value of OTR. This approach is much more reliable than those based on the use of empirical equations. In this chapter, OTR description is discussed and the main experimental methods for k L a determination are described; recent predictive methods for k L a and E are also presented.

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Volumetric oxygen transfer coefficients (kLa) in batch cultivations involving non-Newtonian broths

Cited by 97 publications

References 21 publications

Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed‐batch operation

Fermentation and oxygen transfer characteristics in recombinant human growth hormone production by Pichia pastoris in sorbitol batch and methanol fed‐batch operation

Impact of aeration and agitation on metabolic heat and protease secretion of Aspergillus tamarii in a real-time biological reaction calorimeter

Oxygen Transfer Rate Determination: Chemical, Physical and Biological Methods

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