Voluntary inhalation of methamphetamine: a novel strategy for studying intake non-invasively

Juárez-Portilla, Claudia; Kim, R. D.; Robotham, Margaret; Tariq, Maliha; Pitter, Michael C.; LeSauter, Joseph; Silver, Rae

doi:10.1007/s00213-016-4510-8

Cited by 6 publications

(10 citation statements)

References 48 publications

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“…The efficacy of the nebulized MA in this protocol has been previously demonstrated in several responses ( Juarez-Portilla et al, 2017 ). Mice spend average of ∼3 min in the chamber during the interval of MA availability.…”

Section: Introductionmentioning

confidence: 75%

“…The test apparatus, consisted of a tunnel (7.l × 3.1 × 3.1 cm) connecting the home cage to a nebulization chamber (11.4 × 11.4 × 6 cm), as previously described ( Juarez-Portilla et al, 2017 ). For delivery of vaporized material, a nebulizer (catalog #40-370-000; Briggs Medical Service Company) was attached to the nebulization chamber through a polycarbonate tube.…”

Section: Methodsmentioning

confidence: 99%

“…Elevated locomotor activity occurs during the 1 h of MA availability and for the 3 h thereafter. On the other hand, control mice with access to nebulized water have consistently low activity levels ( Juarez-Portilla et al, 2017 ; Fig. 4 ).…”

Section: Introductionmentioning

confidence: 98%

“…We use a non-invasive protocol that eliminates possible CNS effects of stress associated with handling, injections or surgery that may alter the anticipatory response to the drug. In this protocol, mice live in their home cage and have regularly scheduled daily access to nebulized MA or water for 1h via a tunnel that leads to a chamber where the drug is nebulized and available during their normal sleep time ( Juarez-Portilla et al, 2017 ). Here, we used this protocol to examine behavioral anticipation of MA availability and to identify c-FOS expression at the time of anticipation, before the availability of the drug.…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, following 3-min experimenter-imposed exposure to nebulized MA, serum levels are elevated in mice euthanized 20, 60, or 120 min later. Finally, the amount of time mice spend in the nebulizing chamber is inversely proportional to the concentration of nebulized MA indicating that they self-regulate their intake of MA ( Juarez-Portilla et al, 2017 ; Fig. 5 B ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Brain Activity during Methamphetamine Anticipation in a Non-Invasive Self-Administration Paradigm in Mice

Juárez-Portilla

Pitter

Kim

et al. 2018

eNeuro

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show abstract

Section: Introductionmentioning

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Brain Activity during Methamphetamine Anticipation in a Non-Invasive Self-Administration Paradigm in Mice

Juárez-Portilla

Pitter

Kim

et al. 2018

eNeuro

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Self-administration of methamphetamine aerosol by male and female baboons

Foltin

2018

Pharmacology Biochemistry and Behavior

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The reinforcing efficacy of vaporized methamphetamine HCl (0.3 mg/kg) was determined in baboons with minimal previous drug exposure. A group of 8 adult male baboons was tested prior to a group of 7 adult female baboons. Baboons were initially trained to suck on a brass stem activating a pressure-sensitive relay (i.e., puff), to receive one M&M® candy. Five of the 8 males and 6 of the 7 females learned to activate the relay. 0.05 ml of 95% ethyl alcohol containing 0.3 mg/kg methamphetamine was vaporized and delivered to the mouth of the baboon after he/she completed 2 puffs; a single candy was given after an additional 5 puffs to ensure that baboons continued puffing after the aerosol entered their mouths. Puffing was recorded but not reinforced by candy or drug for 2 min after each aerosol delivery for males and 1 min for females. Males could earn 10 and females could earn 20 aerosol deliveries. Males made between 225 and 650 puffs each session. Females made between 200 and 400 puffs each session. When only candy and placebo aerosol were delivered the number of puffs decreased in all 6 females but increased in all 5 males. When candy was delivered without aerosol, puffing decreased in 4 of 5 males, but this manipulation was not tested in females. Methamphetamine aerosol delivery maintained lower rates of puffing behavior in females than males, but procedural differences weaken interpretation of this sex comparison. Although training non-human primates to inhale drug vapors is time consuming, if successful, their long lifespan could provide years of valuable data justifying further work with non-human primates using models of vaporized drug self-administration.

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Compulsive-Like Sufentanil Vapor Self-Administration in Rats

Vendruscolo

Tunstall

Carmack

et al. 2017

Neuropsychopharmacol.

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Opioid misuse is at historically high levels in the United States, with inhalation (ie, smoking and vaping) being one of the most common routes of consumption. We developed and validated a novel preclinical model of opioid self-administration by inhalation that does not require surgery and reliably produces somatic and motivational signs of dependence. Rats were trained to perform an operant response (nosepoke) to receive 10 s of vaporized sufentanil, a potent opioid, in 2 h daily sessions. Rats readily and concentration-dependently self-administered vaporized sufentanil. Rats exhibited a significant increase in responding for sufentanil when given the preferential μ-opioid receptor inverse agonist naloxone, suggesting the participation of μ-opioid receptors in the reinforcing properties of sufentanil vapor. Serum sufentanil concentrations significantly correlated with the number of sufentanil vapor deliveries. Rats that were given long access (LgA; 12 h/day) but not short access (ShA; 1 h/day) to vaporized sufentanil escalated their drug intake over time and exhibited both naloxone-precipitated somatic signs of opioid withdrawal and spontaneous withdrawal-induced mechanical hypersensitivity. After 6 months of forced drug abstinence, LgA rats returned to pre-escalation baseline levels of responding for sufentanil and mechanical sensitivity. Upon subsequent re-escalation (ie, after the return to extended access to sufentanil vapor), LgA rats again developed naloxone-precipitated somatic signs of withdrawal and spontaneous withdrawal-induced mechanical hypersensitivity. These findings demonstrate that the operant sufentanil vapor self-administration model has both face and construct validity and therefore will be useful for investigating the neurobiological basis of opioid addiction.

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Voluntary inhalation of methamphetamine: a novel strategy for studying intake non-invasively

Abstract: We conclude that administration of MA by nebulization is an effective route of self-administration, and our new protocol represents a promising tool for examining the transitions from first intake to long-term use and its behavioral and neural consequences in a non-invasive protocol.

Cited by 6 publications

References 48 publications

Brain Activity during Methamphetamine Anticipation in a Non-Invasive Self-Administration Paradigm in Mice

Brain Activity during Methamphetamine Anticipation in a Non-Invasive Self-Administration Paradigm in Mice

Self-administration of methamphetamine aerosol by male and female baboons

Compulsive-Like Sufentanil Vapor Self-Administration in Rats

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