von Willebrand Factor Activity Detected in a Monoclonal Antibody-based ELISA: an Alternative to the Ristocetin Cofactor Platelet Agglutination Assay for Diagnostic Use

Murdock, Paul J.; Woodhams, Barry; Matthews, Kathy B; Pasi, K. J.; Goodall, Alison H.

doi:10.1055/s-0038-1657727

Cited by 80 publications

(71 citation statements)

References 28 publications

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“…Measuring vWF using an assay that measures functional activity of the protein was reported to correlate strongly with vWF antigen levels in normal subjects (r = 0.90), and to differentiate between functionally active vWF from degraded antigen [31] . The high levels observed in male subjects imply injury to the endothelium.…”

Section: Discussionmentioning

confidence: 99%

Effects of Cigarette Smoking on Hematological Parameters and von Willebrand Factor Functional Activity Levels in Asymptomatic Male and Female Arab Smokers

Al-Awadhi¹,

AlFadhli²,

Mustafa³

et al. 2008

Med Princ Pract

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Objectives: This study aimed at determining the effects of cigarette smoking based on gender, on several hematological parameters and von Willebrand factor protein in the asymptomatic Arab population of Kuwait. Subjects and Methods: Ninety-two subjects participated in this study: 55 males (31 smokers and 24 nonsmokers) and 37 females (18 smokers and 19 nonsmokers). Complete blood count results were obtained using Beckman Coulter Hematology Analyzer. Von Willebrand factor functional activity was determined using an enzyme-linked immunoassay-based test in which anti-von Willebrand factor IgG monoclonal antibody was used that recognizes a functional epitope of the protein. The coagulation profile was obtained using ACL® 9000 coagulation analyzer. Results: Male smokers had significantly higher levels of white blood cell count (p = 0.03) and von Willebrand factor protein levels (p = 0.029), and a significantly shorter thrombin time (p = 0.019) than nonsmokers. Smoking did not appear to affect any of the parameters analyzed in females as no significant difference was found between smokers and nonsmokers (p > 0.05). Conclusion: Our results showed that smoking affected white blood cell count and von Willebrand factor levels in males and not in females, and as such could be potential markers for smoking-induced endothelial damage in asymptomatic Arab male smokers.

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Section: Discussionmentioning

confidence: 99%

Effects of Cigarette Smoking on Hematological Parameters and von Willebrand Factor Functional Activity Levels in Asymptomatic Male and Female Arab Smokers

Al-Awadhi¹,

AlFadhli²,

Mustafa³

et al. 2008

Med Princ Pract

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“…VWf activity was measured using an Imubind vWf activity ELISA kit (American Diagnostica, Greenwich, CT). The kit employs monoclonal antibody recognizing the epitope of vWf involved in interaction with platelets (27) and represents an alternative to classical RCoF.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Development and Applications of Surface Plasmon Resonance-Based von Willebrand Factor– Collagen Binding Assay

Saenko

Kannicht

Löster

et al. 2002

Analytical Biochemistry

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“…Monoclonal antibody-based ELISA, 3,4 Elisa-based VWF:RCo, 5,6 rapid assays for VWF:RCo using coagulometers, 7 and assays measuring the binding of VWF to collagen. 8 The aim of this study was to evaluate the novel fully automated HemosIL™VWF Activity assay on the ACL-Futura automated coagulometer (Instrumentation Laboratory) as a potential screening test for the diagnosis of VWD.…”

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confidence: 99%

Evaluation of a new turbidimetric assay for von Willebrand factor activity useful in the general screening of von Willebrand disease

Piñol¹,

Sales²,

Costa³

et al. 2007

Haematologica

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We evaluated a new assay (HemosIL™VWF Activity on ACL-Futura) in the screening of VWD. Samples from healthy donors and previously diagnosed VWD patients were blindly analyzed by this new activity assay and standard VWF:RCo. Results agreed and both assays showed a similar sensitivity for the screening of VWD. Haematologica 2007; 92:5: 712-713 Von Willebrand disease (VWD), the most frequent inherited bleeding disorder, is caused by a deficiency and/or abnormality of the von Willebrand factor (VWF).1,2 The revised classification of VWD identifies two major categories, characterized by quantitative (types 1 and 3) or qualitative (type 2) VWF defects.2 The most common test of VWF activity, the assay of VWF:RCo together with VWF:Ag is traditionally considered the first step in the diagnosis of VWD, with the VWF:RCo/Ag ratio being recommended to discriminate type 1 from type 2A, 2B and 2M VWD.1,2 Given the complexities of the aggregometric test, several alternative laboratory methods have been proposed to measure VWF activity. Monoclonal antibody-based ELISA, 3,4Elisa-based VWF:RCo, 5,6 rapid assays for VWF:RCo using coagulometers, 7 and assays measuring the binding of VWF to collagen.8 The aim of this study was to evaluate the novel fully automated HemosIL™VWF Activity assay on the ACL-Futura automated coagulometer (Instrumentation Laboratory) as a potential screening test for the diagnosis of VWD.9,10 The assay uses latex sensitized with monoclonal antibodies (RFF VIII:R/1) 3 directed against the platelet binding site of VWF (GPIb receptor). The activity of VWF is determined by measuring the increase of turbidity produced by the agglutination of the latex particles as a consequence of the interaction between the GPIb receptor of VWF and the monoclonal antibodies. The lower limit of linearity has been reported at 12.5IU/dL. 9 A set of 57 normal controls and 70 VWD patients from two Hemophilia Centers were blindly analyzed in the R&D laboratory of Biokit with HemosIL™VWF:Ag, HemosIL™VWF Activity and VWF:RCo (Dade Behring) performed on an optical aggregometer. All methods were used to analyze samples which were then classified as normal or VWD type 1, 2, or 3 using the same criteria in both activity assays. The cut-off declared by the HemosIL based on ABO group was used to define normality or VWD, and the Activity/Antigen (Act/Ag) ratio was used to define quantitative (type 1) or qualitative (type 2) VWD using 0.7 as cut-off.1 Sample values below detectability were classified as type 3.After classification and database lock, blindness was disclosed by the two Hemophilia Centers where the patient samples were classified according to the clinical and laboratory findings. Table 1 shows the mean VWF concentration in IU/dL±SD (standard deviation) obtained for each assay. The first column shows the Classification according to the Haemophilia Centers. Nine samples from the Vicenza group which correspond to type 1 according to the last classification scheme, 2 are shown separately. The last columns show the mean Act/Ag ratio...

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von Willebrand Factor Activity Detected in a Monoclonal Antibody-based ELISA: an Alternative to the Ristocetin Cofactor Platelet Agglutination Assay for Diagnostic Use

Cited by 80 publications

References 28 publications

Effects of Cigarette Smoking on Hematological Parameters and von Willebrand Factor Functional Activity Levels in Asymptomatic Male and Female Arab Smokers

Effects of Cigarette Smoking on Hematological Parameters and von Willebrand Factor Functional Activity Levels in Asymptomatic Male and Female Arab Smokers

Development and Applications of Surface Plasmon Resonance-Based von Willebrand Factor– Collagen Binding Assay

Evaluation of a new turbidimetric assay for von Willebrand factor activity useful in the general screening of von Willebrand disease

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