Voronoi-based spatial analysis reveals selective interneuron changes in the cortex of FALS mice

Minciacchi, Diego; Kassa, Roman M.; Tongo, Claudia Del; Mariotti, Raffaella; Bentivoglio, Marina

doi:10.1016/j.expneurol.2008.09.005

Cited by 25 publications

(15 citation statements)

References 62 publications

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“…The results described here are of crucial importance to unravel the role of interneurons in the physiopathology of ALS, because 1) a substantial proportion of ALS patients suffers from cognitive impairments, with concomitant hippocampal damage (Abdulla et al, 2014) and display increased anxiety (Kurt et al, 2007); 2) PV expression is altered in several brain regions of ALS patients (Hayashi et al, 2013) and in presymptomatic mSOD1 mice, and these mice also display an increased number of cortical PVi (Sasaki et al, 2006;Minciacchi et al, 2009); 3) hippocampal PVi are damaged in several disorders, including forms of dementia (Mar ın, 2012;Bernstein et al, 2011); 4) PV overexpression in motor neurons delays disease onset and prolongs survival in mSOD1 (Beers et al, 2001); and 5) PVi are necessary for gamma oscillations (Sohal et al, 2009), are crucial for hippocampus-dependent and hippocampusindependent learning Donato et al, 2013), and are involved in the control of anxiety (Hale et al, 2010). Importantly, the hippocampal PVi loss supports and extends previous findings on regional selectivity of PVi alterations in mSOD1 mice ).…”

Section: Discussionmentioning

confidence: 86%

Increased anxiety‐like behavior and selective learning impairments are concomitant to loss of hippocampal interneurons in the presymptomatic SOD1(G93A) ALS mouse model

Quarta

Bravi

Scambi

et al. 2015

J of Comparative Neurology

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Amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease primarily characterized by motor neuron death, causes damages beyond motor-related areas. In particular, cognitive impairments and hippocampal damage have been reported in ALS patients. We investigated spatial navigation learning and hippocampal interneurons in a mutant SOD1(G93A) mouse (mSOD1) model of ALS. Behavioral tests were performed by using presymptomatic mSOD1 mice. General motor activity was comparable to that of wild-type mice in the open-field test, in which, however mSOD1 exhibited increased anxiety-like behavior. In the Barnes maze test, mSOD1 mice displayed a delay in learning, outperformed wild-type mice during the first probe trial, and exhibited impaired long-term memory. Stereological counts of parvalbumin-positive interneurons, which are crucial for hippocampal physiology and known to be altered in other central nervous system regions of mSOD1 mice, were also performed. At postnatal day (P) 56, the population of parvalbumin-positive interneurons in mSOD1 mice was already reduced in CA1 and in CA3, and at P90 the reduction extended to the dentate gyrus. Loss of parvalbumin-positive hippocampal interneurons occurred mostly during the presymptomatic stage. Western blot analysis showed that hippocampal parvalbumin expression levels were already reduced in mSOD1 mice at P56. The hippocampal alterations in mSOD1 mice could at least partly account for the increased anxiety-like behavior and deficits in spatial navigation learning. Our study provides evidence for cognitive alterations and damage to the γ-aminobutyric acid (GABA)ergic system in the hippocampus of murine ALS, thereby revealing selective deficits antecedent to the onset of motor symptoms.

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Section: Discussionmentioning

confidence: 86%

Increased anxiety‐like behavior and selective learning impairments are concomitant to loss of hippocampal interneurons in the presymptomatic SOD1(G93A) ALS mouse model

Quarta

Bravi

Scambi

et al. 2015

J of Comparative Neurology

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“…In motor cortex of SOD1-G93A mice, PV-positive interneurons are abnormally abundant 125 . In light of the reduced PV immunoreactivity reported in human ALS tissue (see above), additional studies are needed to resolve this apparent discordance.…”

Section: Corticostriatal Connectivity In Disease – Evidence Of It/pt mentioning

confidence: 95%

Corticostriatal connectivity and its role in disease

2013

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Corticostriatal projections are essential components of forebrain circuits widely involved in motivated behavior. These axonal projections are formed by two distinct classes of cortical neurons, intratelencephalic (IT) and pyramidal tract (PT) type neurons. Convergent evidence points to IT/PT differentiation of the corticostriatal system at all levels of functional organization, from cellular signaling mechanisms to circuit topology. There is also growing evidence for IT/PT imbalance as an etiological factor in neurodevelopmental, neuropsychiatric, and movement disorders – autism, amyotrophic lateral sclerosis, obsessive-compulsive disorder, schizophrenia, Huntington’s and Parkinson’s diseases, and major depression are highlighted here.

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“…One promising technique is Voronoi diagram analysis, which many researchers have used for identifying spatio-temporal behavior of cell populations. 8,[19][20][21][22] However, the Voronoi diagram technique was insufficient for evaluating the specific behavior of individual cells. In this study, by using comigration analysis, the different cell behavior was evaluated at a cellular level between on fibroin and ProNectin substrates.…”

Section: Discussionmentioning

confidence: 99%

Quantification of Cell Co-Migration Occurrences During Cell Aggregation on Fibroin Substrates

Otaka

Takahashi²,

Takeda³

et al. 2014

Tissue Engineering Part C: Methods

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A quantitative analytical method was proposed for measuring cell co-migration, which was defined as two or more cells migrating together. To accurately identify and quantify this behavior, cell migration on fibroin substrates was analyzed with respect to intercellular distance. Specifically, cell size was characterized by major diameter, and then, based on these measurements and cell center data, a specific threshold distance for defining co-migration was determined after analyzing cell motion using the Voronoi diagram method. The results confirmed that comigration occurrences of rounded cells were significantly more stable on fibroin than on ProNectin substrates under the present experimental conditions. The cell co-migration analysis method in this article was shown to be successful in evaluating the stability of cell co-migration and also suggested the presence of ''critical distance'' where two cells interact on fibroin substrates. With further research, the cell co-migration analysis method and ''critical distance'' may prove to be capable of identifying the aggregation behavior of other cells on different materials, making it a valuable tool that can be used in tissue engineering design.

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Voronoi-based spatial analysis reveals selective interneuron changes in the cortex of FALS mice

Cited by 25 publications

References 62 publications

Increased anxiety‐like behavior and selective learning impairments are concomitant to loss of hippocampal interneurons in the presymptomatic SOD1(G93A) ALS mouse model

Increased anxiety‐like behavior and selective learning impairments are concomitant to loss of hippocampal interneurons in the presymptomatic SOD1(G93A) ALS mouse model

Corticostriatal connectivity and its role in disease

Quantification of Cell Co-Migration Occurrences During Cell Aggregation on Fibroin Substrates

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