Background: The UK national diabetic eye screening (DES) programme invites diabetic patients aged > 12 years annually. Simple and cost-effective methods are needed to increase screening uptake. This trial tests the impact on uptake of two financial incentive schemes, based on behavioural economic principles. Objectives: To test whether or not financial incentives encourage screening attendance. Secondarily to understand if the type of financial incentive scheme used affects screening uptake or attracts patients with a different sociodemographic status to regular attenders. If financial incentives were found to improve attendance, then a final objective was to test cost-effectiveness. Design: Three-armed randomised controlled trial. Setting: DES clinic within St Mary ’ s Hospital, London, covering patients from the areas of Kensington, Chelsea and Westminster. Participants: Patients aged ≥ 16 years, who had not attended their DES appointment for ≥ 2 years. Interventions: (1) Fixed incentive – invitation letter and £10 for attending screening; (2) probabilistic (lottery) incentive – invitation letter and 1% chance of winning £1000 for attending screening; and (3) control – invitation letter only. Main outcome measures: The primary outcome was screening attendance. Rates for control versus fixed and lottery incentive groups were compared using relative risk (RR) and risk difference with corresponding 95% confidence intervals (CIs). Results: A total of 1274 patients were eligible and randomised; 223 patients became ineligible before invite and 1051 participants were invited (control, n = 435; fixed group, n = 312; lottery group, n = 304). Thirty-four (7.8%, 95% CI 5.29% to 10.34%) control, 17 (5.5%, 95% CI 2.93% to 7.97%) fixed group and 10 (3.3%, 95% CI 1.28% to 5.29%) lottery group participants attended. Participants offered incentives were 44% less likely to attend screening than controls (RR 0.56, 95% CI 0.34 to 0.92). Examining incentive groups separately, the lottery group were 58% less likely to attend screening than controls (RR 0.42, 95% CI 0.18 to 0.98). No significant differences were found between fixed incentive and control groups (RR 0.70, 95% CI 0.35 to 1.39) or between fixed and lottery incentive groups (RR 1.66, 95% CI 0.65 to 4.21). Subgroup analyses showed no significant associations between attendance and sociodemographic factors, including gender (female vs. male, RR 1.25, 95% CI 0.77 to 2.03), age ( ≤ 65 years vs. > 65 years, RR 1.26, 95% CI 0.77 to 2.08), deprivation [0 – 20 Index of Multiple Deprivation (IMD) decile vs. 30 – 100 IMD decile, RR 1.12, 95% CI 0.69 to 1.83], years registered [mean difference (MD) – 0.13, 95% CI – 0.69 to 0.43], and distance from screening location (MD – 0.18, 95% CI – 0.65 to 0.29). Limitations: Despite verification, some address details may have been outdated, and high ethnic diversity may have resulted in language barriers for participants. Conclusions: Those receiving incentives were not more likely to attend a DES than those receiving a usual invitation letter...