VP2 Gene of a Canine Parvovirus Isolate from Stool of a Puppy

Hirayama, Kentarou; Kano, Rui; Hosokawa-Kanai, Tomoko; Tuchiya, Koutarou; Tsuyama, Shingo; Nakamura, Yuka; Sasaki, Yoshihide; Hasegawa, Atsuhiko

doi:10.1292/jvms.67.139

Cited by 23 publications

(15 citation statements)

References 16 publications

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“…The second was no evidence of circulation of either CPV2c(a)/2c(b) or "Glu-426" variants. These variants were not detected in previous studies performed in the last decade in Japan [11,12,15,30,32]. The antigenic variants CPV2c(a)/2c(b), first detected from the Vietnamese wild cats, have the substitution of aa residue at position 300 of VP2 from Gly to Asp [18].…”

Section: Discussionmentioning

confidence: 75%

“…The present study revealed two points which were newly clarified for CPV epidemiology in Japan. First, it has clearly demonstrated "replacement from prototype CPV-2a/ 2b to new CPV-2a/2b" in Japanese isolates, although the same fact could be confirmed from the fragmentary data registered in the databases by other studies [15,17,18,29]. However, the key question as to biological significance of the change from prototype CPV-2a/2b to new CPV-2a/2b types remains, because the Ser 297 Ala substitution cannot Fig.…”

Section: Discussionmentioning

confidence: 88%

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Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

et al. 2008

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ABSTRACT. Fifty-five canine parvovirus type 2 (CPV) samples, 12 fecal specimens and 43 cell culture isolates, were examined for their genetic characteristics of VP2 gene. They were collected from the diseased dogs at various districts of Japan during 27 years from 1980 to 2006. A fragment of VP2 gene was analyzed by restriction fragment length polymorphism assay and DNA sequencing. The original antigenic type 2 of CPV (CPV-2) was no longer found in the samples since 1984, and two antigenic variants CPV-2a and CPV-2b replaced CPV-2 as predominant types for about 5 years from 1982. A new genetic variant of prototype CPV-2a with non-synonymous substitution at the VP2 amino acid residue 297 from Ser to Ala was first detected in 1987. New CPV-2b with the same amino acid substitution at position 297 as new CPV-2a was also detected from the samples collected in 1997. Since then new CPV-2b has been the predominant CPV over the field of Japan. Several additional amino acid substitutions were detected in the VP2 gene of some recent CPV strains. Neither CPV-2c(a), CPV-2c(b), nor "Glu-426" of the antigenic variants previously found outside the country was detected in any samples tested. Reactivity of new CPV-2a and 2b variants against antibodies produced by the current vaccine products was determined by a cross hemagglutination-inhibition test. The recent field CPV isolates reacted more efficiently to the antibodies produced in dogs vaccinated with the new CPV-2b vaccine strain than the conventional CPV-2 vaccine strain.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 88%

Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

et al. 2008

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“…3). Rounding and degenerative changes are the cytopathic changes observed 72 h post infection after third passage is characteristic of CPV in CRFK cell lines [12,19]. Of the 18 samples passaged in CRFK cells, 3 samples (1544, 1272 and 3367) which showed mild cytopathic effect also demonstrated high HA titres up to third passage levels.…”

Section: Screening Of Clinical Samples By Pcr Assaymentioning

confidence: 99%

“…The infected monolayers were harvested 4th day post-inoculation (with or without CPE) by three cycles of alternative freezing and thawing. The virus suspension was clarified at 6000 rpm for 15 min in a refrigerated centrifuge and supernatant was frozen at -40°C for further use [12].…”

Section: Isolation Of Cpvmentioning

confidence: 99%

Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India

Parthiban

Mukhopadhyay²,

Panneer³

et al. 2011

Indian J Microbiol

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A total of 128 faecal samples/rectal swabs were collected from dogs showing signs of diarrhea/enteritis in and around Puducherry, South India. Eighteen clinical samples, showing high HA titre of 1:512 and above and positivity by polymerase chain reaction (PCR) with CPV2ab primers, were subjected to virus isolation in CRFK cell line. Of the 18 samples processed, 3 samples (16.6%) were positive for CPV and were confirmed by haemagglutination, dot-ELISA, and IFAT. The three cell culture isolates were characterized as CPV-2b types by multiplex PCR as well as by monoclonal antibody typing.

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“…Virus isolation was carried out as per the procedure recommended by Hirayama et al [24]. Twenty-four PCR positive samples representing the diverse geographical locations of India were randomly selected and subjected for isolation of virus in A-72 cell line maintained in RPMI-1640 media (Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Virus Isolationmentioning

confidence: 99%

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Nookala¹,

Mukhopadhyay²,

Sivaprakasam³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyzed indicating emergence of newer variants of CPV in India. The phylogenetic analysis revealed that all the field CPV types were grouped in different subclades within two main clades, but away from the commercial vaccine strains. CPV-2b and CPV-2c types with unique mutations were found to be establishing in India apart from the prevailing CPV-2a type. Mutations and the positive selection of the mutants were found to be the major mechanism of emergence and evolution of parvovirus. Therefore, the incorporation of local strain in the vaccine formulation may be considered for effective control of CPV infections in India.

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VP2 Gene of a Canine Parvovirus Isolate from Stool of a Puppy

Cited by 23 publications

References 16 publications

Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan

Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

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