Vulnerability of SARS-CoV-2 and PR8 H1N1 virus to cold atmospheric plasma activated media

Abstract

Cold Atmospheric Plasma (CAP) and Plasma Activated Media (PAM) are effective against bacteria, fungi, cancer cells, and viruses because they can deliver Reactive Oxygen and Nitrogen Species (RONS) on a living tissue with negligible damage on health cells. The antiviral activity of CAP against SARS-CoV-2 is being investigated, however, the same but of PAM has not been explored despite its potential. In the present study, the capability of Plasma Activated Media (PAM) to inactivate SARS-CoV-2 and PR8 H1N1 influenza virus with negligible damage on healthy cells is demonstrated. PAM acted by both virus detaching and diminished replication. Furthermore, the treatment of A549 lung cells at different times with buffered PAM did not induce interleukin 8 expression, showing that PAM did not induce inflammation. These results open a new research field by using PAM to the development novel treatments for COVID-19, influenza, and other respiratory diseases.

Anti-amyloidogenic property of gold nanoparticle decorated quercetin polymer nanorods in pH and temperature induced aggregation of lysozyme

“…The calculations were performed using an online tool “Quest GraphTM IC 50 Calculator, AAT Bioquest, Inc.” by using eqn (3). 85,86 The measurements were normalized to the intensity of untreated HEWL amyloid fibrils (taken as 100%).…”

Inactivation of SARS-CoV-2 and Other Enveloped and Non-Enveloped Viruses with Non-Thermal Plasma for Hospital Disinfection

“…Moreover, gas-fed NTP, such as plasma jets, could present additional health risks, as the constant gas flow could blow microdroplets that carry viruses and other contaminating particles into the surrounding environment, thus further spreading its reach. Another strategy which uses NTP for disinfection is via generation of NTP-treated liquids, such as water. , In this modality, NTP is used to treat and enrich a liquid with RONS. This liquid is then applied to the surface to be disinfected.…”

Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages

“…Cortazar et al found that RONS induced by PAM delivers on a living tissue with negligible damage on healthy cells. In contrast, their PAM inactivated SARS-CoV-2 and PR8 H1N1 viruses effectively [ 48 ]. It was reported that the synergistic effect of H 2 O 2 , RONS and a low pH may induce cell death of infected cells.…”