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Shuvalova, S. D.; Gabinskaya, K. N.; Popova, E. V.; Савинова, Т. С.; Andryushina, V. A.

doi:10.1023/a:1011925608119

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…Curvularia lunata strain CBE from the laboratory collection, used for 11b-and 14a-hydroxylation of D 4 -3-ketosteroids [19,20], was stored at a temperature of 4 -5°C on agar slopes containing 4.0 g/liter yeast extract, 10.0 g/liter malt extract, 4.0 g/liter glucose, 30.0 g/liter agar agar, pH 6.2 -6.9. Steroid-transforming activity was studied by transferring cultures 10 -14 days old from agar slopes to 100 ml of medium (in 750-ml conical flasks) consisting of 30.0 g/liter sucrose, 2.5 g/liter yeast autolysate, 2.0 g/liter NaNO 3 , 3.0 g/liter NH 4 H 2 PO 4 , 1.0 g/liter K 2 HPO 4 , 0.5 g/liter KCl, 0.5 g/liter MgSO 4 , pH 6.0 -6.2, and incubated for 65 -70 h at 28°C on a shaker at 220 rpm.…”

Section: Experimental Biological Sectionmentioning

confidence: 99%

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Studies of synthesis routes for biologically active 14α-hydroxylated steroids

et al. 2009

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The conditions for preparing 14a-hydroxyandrostenedione -an intermediate in the synthesis of highly active gestagens -using the fungus Curvularia lunata CBE with an increased steroid substrate load of 5 g/liter in the presence of methylcyclodextrin were identified. The microbiological method of introducing 14a-hydroxy groups into the androstane structure has significant advantages over chemical approaches from the technical and ecological points of view and for obtaining a higher yield.

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Section: Experimental Biological Sectionmentioning

confidence: 99%

“…After recrystallization from acetone, the purified product had a melting temperature of 264°C. The atomic formula was C 19 [21].…”

Section: Experimental Biological Sectionmentioning

confidence: 99%

Studies of synthesis routes for biologically active 14α-hydroxylated steroids

et al. 2009

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Microbial transformation of testosterone byRhizopus stoloniferandFusarium lini

Al‐Aboudi¹,

Mohammad²,

Musharraf³

et al. 2008

Natural Product Research

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The microbial transformation of testosterone by the fungi Rhizopus stolonifer and Fusarium lini has been investigated for the first time. The bioconversion reactions observed from R. stolonifer included oxidation of a 17beta-hydroxyl group, a hydroxylation at equatorial 11alpha position, reduction of the double bond at C-4 with oxidation of the methylene at C-6 to the corresponding keto group, and lactonisation of ring D; the latter is the first report of this reaction by a Rhizopus species. Fusarium lini promoted 1-dehydrogenation of the steroid, which has been rarely observed in fungi cultures. The other routes of biotransformations included oxidation of the 17beta-hydroxyl group and the hydroxylation at 11alpha position. These reactions are not common for Fusarium species.

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Biotransformation of progesterone to hydroxysteroid derivatives by whole cells of Mucor racemosus

Mohamed¹,

El-Refai²,

Sallam³

et al. 2013

MJM

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Aims:The possibility of using Mucor racemosus cells in the biotransformation of progesterone to industrially important hydroxysteroid derivatives in one-step biotransformation process was investigated in this study Methodology and results: The fungal strain was inoculated into the transformation medium which supplemented with PR as a substrate (5-50 mg). The transformation products were separated and characterized on the bases of their GC/MS analysis as 11α-hydroxyprogesterone (11α-HP) as main product (I); 4-pregnen-18-al-11β,21-diol-3,20-dione (Aldosterone) (II) and 20-hydroxy-pregnan-18-oic acid (III) as minor products. The organism was tested for PR bioconversion at different transformation periods (6-96 h), as well as optimization of the basal medium through the addition of different concentrations of yeast extract and peptone (0.5 to 4 g/L) at various pH values (4-9). The optimal biotransformation conditions for maximum production of these PR derivatives were observed using 0.2 g/L of PR, 3 g/L of yeast extract and 3 g/L peptone after 48 h at pH value 5.5. Under these optimal conditions, cells total bioconversion efficiency reached about 96% of the original added PR. Conclusion, significance and impact of study: Under these optimum conditions, M. racemosus has the ability to biotransform PR to 11α-HP (I), Aldosterone (II) and 20-hydroxy-pregnan-18-oic acid (III) with total bioconversion efficiency of 96 ± 1.77%. These results may be of industrial importance because compounds II and III had not been previously recorded as biotransformation products of PR.

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Cited by 4 publications

References 5 publications

Studies of synthesis routes for biologically active 14α-hydroxylated steroids

Studies of synthesis routes for biologically active 14α-hydroxylated steroids

Microbial transformation of testosterone byRhizopus stoloniferandFusarium lini

Biotransformation of progesterone to hydroxysteroid derivatives by whole cells of Mucor racemosus

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