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Davenport, Russell J.; Elliott, James N.; Curtis, Tom; Upton, J.

doi:10.1023/a:1001795609982

Cited by 16 publications

References 20 publications

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Activated Sludge‐Foaming

Soddell

2003

Encyclopedia of Environmental Microbiology

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What is Foaming? Which Microbes Cause Foaming? Use of Molecular Probes in Foam Monitoring Foam Hydrophobicity and Foam Foam Control Foaming in Anaerobic Digesters

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Activated Sludge‐Foaming

Soddell

2003

Encyclopedia of Environmental Microbiology

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What is Foaming? Which Microbes Cause Foaming? Use of Molecular Probes in Foam Monitoring Foam Hydrophobicity and Foam Foam Control Foaming in Anaerobic Digesters

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Rhodococcus

Jones

Goodfellow

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Rho.do.coc'cus. Gr. n. rhodon the rose; N.L. masc. n. coccus (from Gr. masc. n. kokkos grain, seed) coccus; N.L. masc. n. Rhodococcus a red coccus. Actinobacteria / Actinobacteria / Corynebacteriales / Nocardiaceae / Rhodococcus Aerobic, Gram‐stain‐positive to Gram‐stain‐variable, nonmotile actinomycetes that are usually partially acid–alcohol‐fast at some stage of the growth cycle. Rods to extensively branched substrate mycelium may be formed . In all strains the growth cycle begins with the coccus or short rod stage, with different organisms showing a succession of more or less complex morphological stages by which the completion of the cycle is achieved. Cocci may germinate into short rods, form filaments with side projections, show elementary branching, or, in the most differentiated forms, produce extensively branched hyphae. The next generation of cocci and short rods are formed by fragmentation of the rods, filaments, and hyphae. Some strains form sparse microscopically visible aerial hyphae, which may be branched, or aerial synnemata consisting of unbranched filaments that coalesce and project upwards. Colonies may be rough, smooth, or mucoid and pigmented buff, cream, orange, red, or yellow, although colorless variants occur. Chemo‐organotrophic with an oxidative type of metabolism. Catalase‐positive, arylsulfatase‐negative, and sensitive to lysozyme. Most strains grow well on standard media between 15 and 40°C, and use a wide range of organic compounds as sole sources of carbon for energy and growth. DNA G + C content ( mol %): 63–73 (HPLC, T m ). Type species : Rhodococcus rhodochrous (Zopf 1889) Tsukamura 1974, 43 VP .

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Flow cytometric monitoring of Rhodococcus erythropolis and Ochrobactrum anthropi in a mixed culture

Müler

Lösche

Mertingk

et al. 2000

Acta Biotechnologica

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The GRAM-positive bacterium Rhodococcus eryrhropolis K2-3 and the GRAM-negative Ochrobacrrum anrhropi K2-14 are capable of synergistically degrading 4-(2,4-dichlorophenoxy)butyric acid (2,4-DB). The two strains execute this task in a symbiotic manner, but the nature of the interactions involved in the degradation is only partially understood as yet. An essential first step in elucidating the interaction is to be able to monitor the two strains separately, at the cellular level, within mixed populations. Therefore a method exploiting fluorescently labelled lectin probes was developed. Since Concanavalin A (Con A) binds specifically to R.erythropolis K2-3, it was selected and linked to the fluorescent dye Bodipy 630/650, which has an excitation maximum in the red part of the visible light spectrum. Forward light scatter (FSC) and DNA fluorescence from both strains were also measured to obtain simultaneous information about their physiological states. The three parameters were conveniently monitored by dual and triple excitation flow cytometry in conjunction with double fluorescent staining techniques. In addition, the strains were identified using an epifluorescence microscope. These techniques were found powerful tools for the population analysis of this mixed bacterial system.

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Cited by 16 publications

References 20 publications

Activated Sludge‐Foaming

Activated Sludge‐Foaming

Rhodococcus

Flow cytometric monitoring of Rhodococcus erythropolis and Ochrobactrum anthropi in a mixed culture

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