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Fafioye, O.O.; Efuntoye, Moses Olusola; Osho, A.

doi:10.1023/a:1016331418893

Cited by 26 publications

(6 citation statements)

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“…Additionally, isolates from the Fusarium species were found in the samples, indicating that dried fish products were contaminated with Fusarium. These findings are consistent with those published by Nyamwaka et al [15] and Fafioye et al [25], who reported Fusarium contamination of sun-dried fish in Kenya and smoke-dried fish in Nigeria, respectively.…”

Section: Discussionsupporting

confidence: 93%

Assessment of the Fungal Contaminants of Dried Fishes Sold in Open Markets in Awka, Nigeria

Chukwurah,

Ekwealor,

Ekpunobi

2024

Preprint

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Fish is a very healthy food that is a great source of proteins, vitamins, minerals, and essential fatty acids. For many people in developing nations, like Nigeria, smoked and dried fish serves as a significant source of protein and is an integral part of the traditional diet. However, smoked and dried fish are susceptible to fungal growth, especially when the storage conditions are not right, and it has been discovered that many products contain fungi that are easily visible on the surface. This study investigated the diversity and occurrence frequency of contaminated fungi species in dried fishes sold in local markets of Awka in Anambra state, Nigeria. Twenty-four fungal isolates with varying colony morphology were isolated from the various dried fish products. The contaminating species were further subjected to lactophenol cotton blue (LPCB) staining and Ten (10) Unique fungal morphology was detected. Carbohydrate utilization test was used to select unique isolates and further molecular identification showed successful PCR amplification of the isolates. The current study identified various fungal groups contaminating dried fish products sold in Awka Markets. The three dominant genera were the fungal genera' Aspergillus, Penicillium and Fusarium. These results suggest that most dried fish being sold in the Markets are contaminated with multiple fungi, posing a critical health risk to individuals. Thus, there is a need for attention to be paid to the packaging and storage of dried fish to lessen the likelihood of air exposure, lower water activity, and prevent microbiological growth on the dried fish.

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Section: Discussionsupporting

confidence: 93%

Assessment of the Fungal Contaminants of Dried Fishes Sold in Open Markets in Awka, Nigeria

Chukwurah,

Ekwealor,

Ekpunobi

2024

Preprint

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“…This can lead to fungal contamination because of the unhygienic drying conditions. In recent years, several studies have reported the serious fungal load in dried seafood products, especially in dried fish [ 5 , 6 ]. The common fungal genera isolated from contaminate dried fish are Aspergillus sp., Penicillium sp., Rhizopus sp., and Fusarium sp.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Aflatoxin B1, T-2 Toxin, Ochratoxin A and Deoxynivalenol in Dried Seafood Products by LC-MS/MS

Deng

Wang

Deng

et al. 2020

Toxins

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Mycotoxins are secondary metabolites produced by fungi. These contaminate dried seafoods during processing and storage and represent a potential health hazard for consumers. A sensitive, selective and accurate liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was established for simultaneous quantification of four common mycotoxins (aflatoxin B1 (AFB1), T-2 toxin (T-2), ochratoxin A (OTA) and deoxynivalenol (DON)) in dried shrimp, dried fish and dried mussel products. Mycotoxins were extracted from dried seafood samples by acetonitrile/water (85/15, v/v), subjected to ultrasound for 60 min at 20 °C and cleaned up by defatting with n-hexane. The sample matrix affected the linearity of detection (R2 ≥ 0.9974). The limit of detection (LOD) and limit of quantification (LOQ) in dried seafood products varied from 0.1 to 2.0 µg·kg−1 and 0.3 to 5.0 µg·kg−1, respectively. The method was validated by spiking samples with specific mycotoxin levels, and the recoveries, intra-relative standard deviation (RSDs) and inter-RSDs ranged between 72.2–98.4%, 2.8–10.6%, and 5.5–15.4%, respectively. This method was used to analyze 40 dried seafood products purchased from the Zhanjiang seafood market. Results of this product sampling showed that while no DON was detected, AFB1, T-2 and OTA were detected in 30.8%, 17.5% and 33.3% of the samples, respectively. AFB1, T-2 and OTA concentrations varied at 0.58–0.89, 0.55–1.34 and 0.36–1.51 µg·kg−1, respectively. Relatively high frequency of contamination and the presence of AFB1, OTA and T-2 residues indicate the need to monitor mycotoxins in dried seafood products.

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“…Because of some activities, which are seen regularly such as, the building of toilets on water bodies and the discharge of untreated and improperly treated sewage into water bodies, high levels of temperature [11,12] and high concentrations of heavy metals [13] in water bodies, there is therefore need to check if there are isolates of E. coli from water bodies in Rivers State that have developed mutations.…”

Section: Introductionmentioning

confidence: 99%

Using Molecular Techniques for the Characterization of Escherichia coli Isolated from Creek Road/Bonny Estuary in Rivers State, Nigeria

Aleru

Wachukwu

2020

JAMB

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This study characterised Escherichia coli isolated from Creek Road/Bonny Estuary using molecular techniques. One hundred and twenty (120) water samples were investigated for the presence of E. coli in Creek Road/Bonny Estuary. Most Probable Number (MPN) Technique, Eijkman test and molecular techniques were used for the isolation and detection of E. coli. The analysis was carried out periodically (morning and evening) and seasonally (end of rainy season (November), the start of rainy season (April) and in the middle of rainy season (July)). All E. coli isolates were screened for resistance genes in the pathotypes of E. coli (EHEC, EPEC, EAEC, ETEC and EIEC), including Sulfhydryl Variable (SHV), CTX-M, Temoniera (TEM) and MCR genes using the Polymerase chain reaction Technique. The results showed that 100% of the water samples were positive for coliforms and all the water samples harboured E. coli. The highest total coliforms count recorded was in the morning of April (48 MPN/100 ml). The distribution of individual genes in the E. coli isolates are: SHV (5.0), CTX-M (5.8), TEM (4.2), MCR (0.0%), stx1 and stx2 (4.2), esV and bfA (1.7), aaiC (4.2), elt (3.3) and invE (2.5). The CTX-M was the most found of all the resistance genes.

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Cited by 26 publications

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Assessment of the Fungal Contaminants of Dried Fishes Sold in Open Markets in Awka, Nigeria

Assessment of the Fungal Contaminants of Dried Fishes Sold in Open Markets in Awka, Nigeria

Simultaneous Quantification of Aflatoxin B1, T-2 Toxin, Ochratoxin A and Deoxynivalenol in Dried Seafood Products by LC-MS/MS

Using Molecular Techniques for the Characterization of Escherichia coli Isolated from Creek Road/Bonny Estuary in Rivers State, Nigeria

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