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Dabauza, Mercedes; Peña, Leandro

doi:10.1023/a:1017585407870

Cited by 37 publications

(16 citation statements)

References 21 publications

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“…The range of days to growth initiation was much smaller for cloned material in this experiment (10-28 days) as compared to previously reported seed germination ranges of 10-47.4 days [22]. Our data supports previous research showing that somatic regeneration of pepper is feasible [24,25]. Plant growth proceeded normally in all cloned material.…”

Section: Resultssupporting

confidence: 88%

Aeroponic Cloning of Capsicum spp.

et al. 2019

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Aeroponic cloning is a great strategy to maintain desired genotypes by generating a whole new plant from cuttings. While this propagation technique has been demonstrated for tomatoes (Solanum lycopersicum) and potatoes (Solanum tuberosum), no protocol has been developed for peppers (Capsicum spp.). The ability to clonally propagate different Capsicum holds promise for domestic and industrial growing operations since elite cultivars with desirable traits (e.g., high capsaicin levels, nutrient content, and striped fruit) can be perpetuated without the need of planning a nursery. We tested six Capsicum species for their feasibility of aeroponic cloning by stem cuttings. All domestic species were successfully regenerated under aeroponic conditions but not for Capsicum eximium, a wild species. Of the species analyzed, Capsicum annuum peppers had the fastest node formation (11.6 +/− 0.89 days, P ≤ 0.01) and obtained a larger volume of roots (P ≤ 0.01) after node formation as compared to C. baccatum, C. frutescens, and C. pubescens. This study presents a cost-effective strategy to clonally propagate peppers for personal, industrial, and conservation purposes.

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Section: Resultssupporting

confidence: 88%

Aeroponic Cloning of Capsicum spp.

et al. 2019

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“…The possibility of vegetative in vitro mass propagation of pepper can overcome the difficulties listed above, providing an unlimited number of entirely homogeneous, healthy in vitro plantlets, independently of season (Song et al, 2010;Otroshy et al, 2011). According to Dabauza and Peña (2001), pepper micropropagation is a unique tool that is very convenient for maintaining the genetic homogeneity of the elite plant materials, as well as for preserving heterozygous genotypes and genetically transformed forms. Additionally, the discussed method facilitates the gathering and management of pepper germplasm collections.…”

Section: Introductionmentioning

confidence: 99%

“…The numerous experiments that followed demonstrated that the effectiveness of plant micropropagation in different species of pepper (Capsicum spp.) varies depending on the plant genotype, the type of initial explant, and the factors and conditions of in vitro cultures (Phillips and Hubstenberger, 1985;Rajam, 1994, 1996;Dabauza and Peña, 2001). In consequence, effective methods have been developed for specific cultivars that recommend necessary modifications for particular genotypes (Dabauza and Peña, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…varies depending on the plant genotype, the type of initial explant, and the factors and conditions of in vitro cultures (Phillips and Hubstenberger, 1985;Rajam, 1994, 1996;Dabauza and Peña, 2001). In consequence, effective methods have been developed for specific cultivars that recommend necessary modifications for particular genotypes (Dabauza and Peña, 2001). In general, it was found that both 6-benzylaminopurine (BA) and indole acetic acid (IAA) are effective growth regulators in the first stage of organogenesis induction (Phillips and Hubstenberger, 1985;Rajam, 1994, 1996;Gatz, 2002b;Paz and Castañeda, 2004;Qin et al, 2005;Sanatombi and Sharma, 2007).…”

Section: Introductionmentioning

confidence: 99%

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In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Orlinska¹,

Nowaczyk²

2015

Turk J Biol

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This study evaluates the effectiveness of in vitro regeneration of 4 genotypes of pepper (Capsicum spp.) that differ in origin and functional properties: California Wonder, (ATZ × Sono)F 1 , Jalapeno, and SF-9. In order to investigate the effect of the initial explant type, photoperiod, and presence of 2.0 mg L -1 of glycine in the medium, organogenesis was induced on the MS medium with 2.0 g L -1 2-(N-morpholine) ethanesulfonic acid, 1.7 mg L -1 AgNO 3 , 0.4 mg L -1 indole-3-acetic acid (IAA), and 6.0 mg L -1 6-benzyladenine. The effectiveness of organogenesis in the 3 genotypes representing C. annuum L. was similar and considerably lower for line SF9 derived from an interspecific hybrid (C. frutescens L. × C. annuum L.). The highest number of adventitious leaves was recorded in the decapitated seedlings exposed to continuous light. The lack of glycine in the medium favored direct organogenesis. A simultaneous formation of adventitious leaves with a stable number of chromosomes and mixoploid callus tissue was determined by flow cytometry. The successive stage of foliaged shoots and roots formation was most effective in the MS medium with 1.1 mg L -1 IAA. The use of these optimal conditions resulted in developing 42%-56% of properly formed diploid plantlets, successfully acclimatized in 86%-97%.

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“…The major bottlenecks in Capsicum regeneration are the general low frequency of shoot formation and the development of malformed shoot buds and shoots (variously referred to in the literature as rosette shoots, leafy shoots or blind leaves) that fail to elongate, most likely due to the absence of a shoot apical meristem (Dabauza and Pena 2003; Engler et al 1993; Kothari et al 2010; Liu et al 1990; Mihálka et al 2003; Wolf et al 2001). In general, the chili (hot) pepper types are much more responsive for in vitro regeneration than the sweet pepper types (Dabauza and Pena 2001; Engler et al 1993; Khan et al 2006; Lopez-Puc et al 2006; Ochoa-Alejo and Ramirez-Malagon 2001; Solís-Ramos et al 2010; Zapata-Castillo et al 2007) although a strong genotype dependency has been observed in both.…”

Section: Introductionmentioning

confidence: 99%

Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor

et al. 2011

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Pepper (Capsicum L.) is a nutritionally and economically important crop that is cultivated throughout the world as a vegetable, condiment, and food additive. Genetic transformation using Agrobacterium tumefaciens (agrobacterium) is a powerful biotechnology tool that could be used in pepper to develop community-based functional genomics resources and to introduce important agronomic traits. However, pepper is considered to be highly recalcitrant for agrobacterium-mediated transformation, and current transformation protocols are either inefficient, cumbersome or highly genotype dependent. The main bottleneck in pepper transformation is the inability to generate cells that are competent for both regeneration and transformation. Here, we report that ectopic expression of the Brassica napus BABY BOOM AP2/ERF transcription factor overcomes this bottleneck and can be used to efficiently regenerate transgenic plants from otherwise recalcitrant sweet pepper (C. annuum) varieties. Transient activation of BABY BOOM in the progeny plants induced prolific cell regeneration and was used to produce a large number of somatic embryos that could be converted readily to seedlings. The data highlight the utility of combining biotechnology and classical plant tissue culture approaches to develop an efficient transformation and regeneration system for a highly recalcitrant vegetable crop.Electronic supplementary materialThe online version of this article (doi:10.1007/s00299-011-1018-x) contains supplementary material, which is available to authorized users.

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Untitled

Cited by 37 publications

References 21 publications

Aeroponic Cloning of Capsicum spp.

Aeroponic Cloning of Capsicum spp.

In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor

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