Walnut-derived peptide PPKNW alleviate polystyrene microparticles-induced growth inhibition of Lactobacillus rhamnosus GG

Xu, Yongzhao; Nie, Shiying; Wang, Min; Zhao, Zikuan; Amakye, William Kwame; Yuan, Erdong; Ren, Jiaoyan

doi:10.1016/j.fbio.2023.102528

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…A label‐free nuclear magnetic resonance method has demonstrated that the mandatory amino acid residues in the peptide sequence are critical for the specific binding between ZnO and peptides (Rothenstein et al., 2012). However, walnut peptide PW5 had been found to interact with microplastic through hydrogen bonding and van der Waals forces that could reduce the inhibitory effect of microplastic on LGG growth (Xu et al., 2023). However, the field of nanomaterials interaction with food matrixes (food‐derived peptides) is still young, and the possible mechanisms remains to be determined in the future work.…”

Section: Resultsmentioning

confidence: 99%

Effect of walnut peptide‐ZnO nanocomposites on the colon adhesion behavior of Lactobacillus rhamnosus LRa05

et al. 2023

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When the nanoparticles (NPs) in food contact materials are exposed, they may be ingested with the food matrix, resulting in unknown impacts. Here, the biological response of the nanocomposites of nano zinc oxide (nZnO) and walnut protein‐derived peptides (i.e., PW5, WN5, AE6, and WE7) on the Lactobacillus rhamnosus LRa05 growth and adhesion was studied. In an in vitro mouse intestinal adhesion model, we first spotted that the probiotics LRa05 primarily adhered to and colonized the colonic segment. nZnO effectively inhibited the growth and adhesion properties of LRa05 at high concentrations (≥ 1000 μg/mL). Fortunately, when compared to the individual nZnO, the nZnO‐walnut‐derived peptides nanocomposites significantly increased the growth of LRa05. It was found that the alterations in the adhesion ability of LRa05 after treatment with various substances (nZnO and nanocomposites of nZnO‐walnut peptides) were related to the auto‐aggregating property on the LRa05 surface. These results shed light on the effect of food matrices on the safety of nanomaterials in food, and they may have far‐reaching implications for the use of nanomaterials in the food industry.

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Section: Resultsmentioning

confidence: 99%

Effect of walnut peptide‐ZnO nanocomposites on the colon adhesion behavior of Lactobacillus rhamnosus LRa05

et al. 2023

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“…The fluorescence intensities at various temperatures were used to substitute into eq , respectively, and the quenching constant K SV was derived from the slope. Furthermore, the fluorescence intensities at different temperatures were substituted into eq , and the binding constants K b and the number of binding sites n were obtained from the slope and intercept, respectively log [ false( F 0 − F false) / F ] = lg K normalb + nlg [ Q ] In eq , F 0 is the fluorescence intensity of the peptide, F is the fluorescence intensity of the peptide when nZnO with the concentration of [Q] is added, K b is the apparent binding constant of nZnO and the peptide, n is the number of binding sites, and [Q] is the concentration of nZnO addition.…”

Section: Methodsmentioning

confidence: 99%

“…The type of interaction force could be judged according to the thermodynamic parameters Δ G , Δ H , and Δ S , which were calculated according to the following formula: normalΔ G = normalΔ H − T normalΔ S = prefix− italicRT .25em ln .25em italicK normalb In eq , R is the molar gas constant with a value of 8.314 J/(mol·K), T is the Kelvin temperature (K), and K b is the apparent binding constant.…”

Section: Methodsmentioning

confidence: 99%

Peptides with Charged Amino Acids Mitigate nZnO-Induced Growth Inhibition of Lactobacillus rhamnosus LRa05

Ouyang,

Nie,

Yang

et al. 2023

J. Agric. Food Chem.

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Growing concern is about the potential side effects of nanomaterials from food packaging, notably zinc oxide nanoparticles (nZnO). Previous research revealed that walnut-derived peptides could mitigate this inhibitory effect, but the mechanism involved is unclear. Here, we found that not all peptides have such an effect. Based on the growth inhibition model of Lactobacillus rhamnosus LRa05 induced by nZnO, we assessed the protective effects of various peptides. Notably, four peptides containing charged amino acids (PPKNW, WPPKN, ADIYTE, and WEREEQE) were found to effectively alleviate the growth inhibition phenomenon. We hypothesize that the peptide−nZnO interaction modifies this effect, as confirmed through infrared, Raman, and fluorescence spectroscopy. Our results highlight amide bonds, amino groups, carboxyl groups, and benzene rings as key peptide binding sites on nZnO, with static quenching primarily due to hydrogen bonds and van der Waals forces. This study elucidates peptide characteristics in nZnO interactions, facilitating a deeper exploration of food matrix−nanocomposite interactions.

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Effects of partial reduction of polystyrene micro-nanoplastics on the immunity, gut microbiota and metabolome of mice

Zha,

Tang,

et al. 2024

Chemosphere

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Walnut-derived peptide PPKNW alleviate polystyrene microparticles-induced growth inhibition of Lactobacillus rhamnosus GG

Cited by 6 publications

References 20 publications

Effect of walnut peptide‐ZnO nanocomposites on the colon adhesion behavior of Lactobacillus rhamnosus LRa05

Effect of walnut peptide‐ZnO nanocomposites on the colon adhesion behavior of Lactobacillus rhamnosus LRa05

Peptides with Charged Amino Acids Mitigate nZnO-Induced Growth Inhibition of Lactobacillus rhamnosus LRa05

Effects of partial reduction of polystyrene micro-nanoplastics on the immunity, gut microbiota and metabolome of mice

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