Walnut Micropropagation : First Results

Gruselle, R.; Badia, N.; Boxus, P.

doi:10.17660/actahortic.1987.212.78

Cited by 23 publications

(14 citation statements)

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“…However, BA was also effective for the induction of shoot proliferation with proliferation frequencies of up to 88% being possible for shoot tip explants. BA has generally been used in combination with an auxin, typically IBA, for walnut micropropagation (Rodriguez et al 1989); and for J. regia taxa, IBA supplementation has been demonstrated to have positive effects on shoot culture growth (Rodriguez 1982;Driver and Kuniyuki 1984;Gruselle et al 1987;Saadat and Hennerty 2002). In the current study, IBA (0.05 μM) facilitated both elongation and proliferation, depending on the explant type, but the improvements were generally not statistically significant.…”

Section: Discussionmentioning

confidence: 52%

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Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: evaluation of multiple nutrient formulations and cytokinin types

Bosela

Michler

2008

In Vitro Cell.Dev.Biol.-Plant

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The growth of black walnut shoot cultures was compared on media differing in nutrient formulation (MS, DKW, WPM, and 1/2X DKW), cytokinin type (ZEA, BA, and TDZ), and cytokinin concentration. On WPM and 1/2X DKW media, hyperhydricity was observed at frequencies of 60-100% compared with frequencies of 10-40% on the high-salt media (DKW and MS). All three cytokinins facilitated shoot regeneration from nodal cuttings, but recurrent elongation was only observed for BA (5-12.5 μM) and ZEA (5-25 μM) with mean shoot heights of 70-80 mm being possible after two culture periods (6-8 wk) for the fastest elongating lines. ZEA was effective across all six shoot lines with mean shoot heights of at least 35 mm over two culture periods, but two of the shoot lines were 'nonresponsive' to BA with mean shoot heights of <15 mm. In contrast, when shoot tip explants were used for culture multiplication, ZEA was the least effective cytokinin with proliferation frequencies of only 30-40%. The proliferation frequencies were twice as great (75-87%) for TDZ (0.05-0.1 μM), but most of the shoots regenerated were swollen or fasciated in morphology. High rates of proliferation (61-88%) were also possible using BA (12.5-25 μM), but axillary shoots did not elongate well, growing to heights of only 5-10 mm, on average, after 4-5 wk. Since the cytokinin types and concentrations required for high-frequency (>50%) axillary proliferation had adverse effects on the morphology and growth potential of the shoots, multiplication strategies based on the use of nodal cuttings are recommended.

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Section: Discussionmentioning

confidence: 52%

“…In contrast, for J. regia media dilution has been observed to have adverse effects on shoot growth (Cossio and Minolta 1983;Gruselle et al 1987;Amiri 2006;Fatima et al 2006). Although most of the shoots regenerated on 1/2X DKW medium were hyperhydric (90-100%; Table 1), these problems may be unique to nodal cutting explants.…”

Section: Discussionmentioning

confidence: 99%

Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: evaluation of multiple nutrient formulations and cytokinin types

Bosela

Michler

2008

In Vitro Cell.Dev.Biol.-Plant

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“…These result are in agreement with what had been found by Pontikis and Spoutzaki (1984) and Pasqual and Audo (1989) that using of cytokinins and auxin in this category is very important and the role of cytokinins at this stage is to help the meristems grow and form a vegetative shoot by balancing with auxins auto produced by the meristem. (Gruselle et al, 1987). Whereas, the effect of IAA did not differ significantly from the other treatment concerning number of shoot, while in case of leaves number the concentration of IAA 0.2mgl -1 gave the highest value (2.6 leaves/explant).…”

Section: Plant Materials [Source Of Explants and Explant Preparation]mentioning

confidence: 65%

MICROPROPAGATION OF GARDENIA Gardenia jasminoides

M.S.Duhoky¹

2010

Mesopotamia Journal of Agriculture

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The present study was carried out to study the micropropagation of Gardenia (Gardenia jasminoides) by using single nodes excised from soft cuttings using half strength MS salts, 30gl -1 sucrose, 7gl -1 Agar and different concentrations of plant growth regulators in culture medium. Results of the experiment at initiation stage revealed that the culture of single nodes of Gardenia on a medium containing 5mgl -1 BA gave the highest number of shoots (2.2 shoots/explant). Concerning the interaction, a nutrient medium containing (2 mgl -1 BA+ 0.4 mgl -1 IAA) gave the highest values of average number of shoots and leaves and length of new shoots (1.6 shoots/explant, 2 leaves/explant, 1.6cm respectively). At vegetative multiplication stage, the results showed that the medium supplemented by 2 mgl -1 BA gave the highest values of average number of shoots and leaves and length of new shoots (2.20 shoots/explant, 3.20 leaves/explant, 1.80cm respectively), whereas the medium supplemented by 0.3 mgl -1 IAA gave the highest number of shoots and length of new shoots. In order to promote the shoots produced at vegetative multiplication stage to root, it was noticed that the treatment of 8mgl -1 NAA gave the highest percentage of rooting (90%) and the medium supplemented by 12mgl -1 IAA gave (100%) rooting. Plantlets obtained were transferred to pots and acclimatized with 95 % success.

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“…Microclonal propagation of walnut is one such method. The results of research by a number of scientists [4][5][6][7][8][9] demonstrated the feasibility of using tissue culture for Paradox mass reproduction (J. hindsi x J. regia) and to apply this process on a larger scale to ensure commercial demand for walnut seedlings. They obtained good results on the microclonal propagation of walnuts using DKW growth medium with 1.0 mg / L benzyladenine (BA) and 0.001 mg / L Indolylbutyric acid (IBA).…”

Section: Influence Of Nutrition Compositions On Microclonal Propagation Different Genotypes Of the Walnut Juglans Regia Lmentioning

confidence: 99%

Influence of Nutrition Compositions on Microclonal Propagation Different Genotypes of the Walnut Juglans Regia L.

Yegizbayeva

Yausheva²,

Oleichenko

et al. 2020

The Bulletin

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Walnut is one of the most common nuts in many countries of the world. It is used in cooking and in traditional medicine. Walnut cultivation on an industrial scale in Kazakhstan began only in 2016-2017, so Kazakhstan scientists in this field do not yet have any scientific experience. The article presents the results of studies on the influence of the nutrient medium compositions Murashige-Skoog and Driver-Kunzhuki on the microclonal propagation of different genotypes of the walnut Juglans regia L. Two varieties were selected as mother plants: the Uzbek variety Ideal and the Chinese variety Liaohe-1, adapted in the southeast Kazakhstan. In the course of work, we compared the growth rate of walnut shoots of two varieties on nutrient media Murasige-Skoog (MS) and Driver-Kunzhuki (DKW) with a concentration of 6-benzylaminopurine (BAP) of 1 and 1.5 mg / l. After a month of cultivation of shoots, positive growth dynamics was observed only on MS medium. On plants of both varieties, the growth rate was slow on DKW medium, the plants had yellow leaves, some shoots blackened and perished. To obtain high-quality material, as well as to increase the growth rate of shoots, DKW medium was supplemented with FeEDDHA (119 mg / L) and phloroglucinol (50 mg / L). As a result of phenological observations: the height of the main shoot, the number of internodes, the formation of additional shoots, and the state of the plants, it was found that the best nutrient medium for microclonal propagation of walnut Ideal and Liaohe-1 is a modified DKW medium. MS can only be used at the initial stage - an introduction to in vitro culture.

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Walnut Micropropagation : First Results

Cited by 23 publications

References 0 publications

Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: evaluation of multiple nutrient formulations and cytokinin types

Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: evaluation of multiple nutrient formulations and cytokinin types

MICROPROPAGATION OF GARDENIA Gardenia jasminoides

Influence of Nutrition Compositions on Microclonal Propagation Different Genotypes of the Walnut Juglans Regia L.

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