Wangia profunda gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from southern Okinawa Trough deep-sea sediment

Abstract

An orange-pigmented, Gram-negative, nonmotile, strictly aerobic and oxidase- and catalase-positive bacterium (SM-A87(T)) was isolated from the deep-sea sediment of the southern Okinawa Trough area. The main fatty acids were i15 : 0, i17 : 0 3OH, i15 : 1 G, i17 : 1 omega 9c, 15 : 0, i15 : 0 3OH and summed feature 3 (comprising i-15 : 0 2OH and/or 16 : 1 omega 7c). MK-6 was the predominant respiratory quinone. DNA G+C content was 35.8 mol%. Flexirubin-type pigments were absent. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SM-A87(T) formed a distinct lineage within the family Flavobacteriaceae, with <93% sequence similarity to the nearest strain of genus Salegentibacter. Moreover, strain SM-A87(T) could be distinguished from the nearest phylogenetic neighbors by a number of chemotaxonomic and phenotypic properties. On the basis of polyphasic analyses, it is proposed that strain SM-A87(T) be classified in a novel genus and a new species in the family Flavobacteriaceae, designated Wangia profunda gen. nov., sp. nov. The type strain is SM-A87(T) (CCTCC AB 206139(T)=DSM 18752).

Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough

“…Several isolates, such as K2-28-058, K0-28L-010, K6-28L-034, K2-28-011 and the closely related strains, have only moderate 16S rDNA sequence similarity (94-97%) to their GenBank best match sequences from taxonomically well determined bacteria species, indicating that they may be new species or even new genus. The strain K2-28-058 was eventually identified as a new species in a new bacterial genus, Wangia profunda (Qin et al 2007). Most of the 16S rDNA GenBank nearest neighboring sequences of our isolates were originally obtained from deep-sea sediment environments, consistent to the in situ environmental characteristics of our isolates.…”

Comparative genomics reveals a deep-sea sediment-adapted life style of Pseudoalteromonas sp. SM9913

“…The antibiotics contained within one disc were: kanamycin, 30 mg; tetracycline, 30 mg; penicillin, 1 mg; ampicillin, 10 mg; amoxicillin, 10 mg; and chloromycetin, 30 mg. Chitin degradation ability was tested according to Cottrell et al (2000), that is, by observing cleared zones. Other physiological and biochemical properties were tested using the commercial systems API 20E, API ZYM (both from bioMerieux), following the manufacturer's instructions with some modifications (Qin et al, 2007b). Flagella of cells grown in marine Luria-Bertani broth or on 0.3% agar were observed by transmission electron microscopy (JEM-100CXII).…”

“…Swimming motility was determined using the hanging drop method (Qin et al, 2007b). Sensitivity to antibiotics was tested using the disc-diffusion method, as described previously (Qin et al, 2007b). The antibiotics contained within one disc were: kanamycin, 30 mg; tetracycline, 30 mg; penicillin, 1 mg; ampicillin, 10 mg; amoxicillin, 10 mg; and chloromycetin, 30 mg. Chitin degradation ability was tested according to Cottrell et al (2000), that is, by observing cleared zones.…”

“…The experiments were repeated twice. Swimming motility was determined using the hanging drop method (Qin et al, 2007b). Sensitivity to antibiotics was tested using the disc-diffusion method, as described previously (Qin et al, 2007b).…”

Characterization of Bacterial Polysaccharide Capsules and Detection in the Presence of Deliquescent Water by Atomic Force Microscopy

“…In this report, we selected the CPS-producing bacterium Zunongwangia profunda SM-A87 (formerly named Wangia profunda SM-A87) (16) for the capsule research. The strain was cultured as previously described (11).…”