Water-associated attributes in the contemporary dentin bonding milieu

Pucci, César Rogério; Gu, Li sha; Zhang, Hong yun; Song, Qun; Xia, Veronica; Davis, Linsey B.; Andrade, Danilo de Souza; Mazzoni, Annalisa; Breschi, Lorenzo; Pashley, David H.; Tay, Franklin R.; Niu, Li Na

doi:10.1016/j.jdent.2018.04.016

Cited by 23 publications

(27 citation statements)

References 53 publications

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“…Permeability evaluation was conducted on resin-dentin interfaces bonded with 20-cm water pressure (Pucci et al 2018). Water permeability setup and examination procedures are included in the Appendix.…”

Section: Methodsmentioning

confidence: 99%

Chitosan-Based Extrafibrillar Demineralization for Dentin Bonding

Cai

Guo

et al. 2018

J Dent Res

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Instability of resin-dentin bonds is the Achilles’ heel of adhesive dentistry. To address this problem, a chelate-and-rinse extrafibrillar dentin demineralization strategy has been developed that keeps intrafibrillar minerals within collagen fibrils intact to prevent activation of endogenous proteases that are responsible for collagen degradation within hybrid layers. The objective of the present study was to evaluate the potential of using chitosan >40 kDa as an antimicrobial extrafibrillar dentin-chelating agent to enhance bond durability. Transmission electron microscopy provided evidence for retention of intrafibrillar minerals and smear plugs in dentin conditioned with 1 wt% chitosan. Analyzed by Kruskal-Wallis analysis of variance, Dunn’s statistic, and separate Mann-Whitney tests, tensile bond strengths to wet- and dry-bonded dentin indicated that chelating dentin with chitosan for 60 s prior to bonding did not result in a significant decline in resin-dentin bond strength when compared with that of phosphoric acid etching ( P > 0.05). Gelatinolytic activity within the hybrid layers was examined via in situ zymography after 24-h storage or after thermomechanical cycling and analyzed with 3-factor analysis of variance. After 24 h, enzymatic activity was detected only within completely demineralized phosphoric acid–etched dentin, with values derived from dry bonding significantly higher than those derived from wet bonding ( P < 0.05). Negligible fluorescence was detected within hybrid layers when dentin was conditioned with chitosan, even after thermomechanical cycling, as compared with the controls. Reduction in water permeability in chitosan-conditioned dentin, attributed to smear plug retention, also fostered long-term bond stability. Antibacterial testing performed with live/dead staining indicated that the acetic acid–solubilized chitosan possessed antibacterial activities against 3 single-species biofilms: Streptococcus mutans, Actinomyces naeslundii, and Enterococcus faecalis. Taken together, the new chitosan-based extrafibrillar demineralization strategy retains intrafibrillar minerals, reduces endogenous protease-initiated collagen degradation, prevents water permeation within hybrid layers, and kills bacteria on dentin surfaces, which are crucial factors for enhancing resin-dentin bond durability.

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Section: Methodsmentioning

confidence: 99%

Chitosan-Based Extrafibrillar Demineralization for Dentin Bonding

Cai

Guo

et al. 2018

J Dent Res

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“…In vitro bonding was performed using 20 cm H 2 O of stimulated hydrostatic pulpal pressure (Appendix Fig. 6) (Pucci et al 2018). The crown enamel was removed parallel to the occlusal surface under water cooling.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a Collagen-Reactive Monomer with Advanced Bonding Durability

Fan

Luo

et al. 2020

J Dent Res

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This study evaluated the use of a new collagen-reactive monomer (CRM), isocyanate-terminated urethane methacrylate precursor, which has covalent affinity to dental collagen, in the formation of dentin-resin bonds and compared it with 2 other dental adhesives. Dentin specimens were bonded with either the CRM-based adhesive (CBA), One-Step (OS; Bisco, Inc.), or a negative adhesive (NA) control and subjected to 24-h storage in water, thermocycling to simulate 1-y clinical function, or a matrix metalloproteinase–mediated aging process. We tested the microtensile bond strength (µTBS), characterized the bonding interface with an atomic force microscope, conducted micro-Raman analysis, and performed leakage tests and in situ zymography. CBA and OS exhibited comparable bonding strength after 24 h ( P > 0.05); however, there was a sharp decrease in µTBS after aging for all except CBA ( P < 0.001). Raman spectra results indicated increased collagen crosslinking and chemical reaction between the adhesive and collagen in the CBA group. CBA achieved high-quality hybridization with collagen, improving mechanical properties and integrity, and decreased the enzyme-mediated degradation of the bonding interface by inhibiting collagenolytic activity. With the promising bonding durability of coapplied CBA, CRM may be the first dental adhesive to provide strong and long-lasting resin-dental collagen bonding without the additional conditioning step. The use of CBA results in high-quality hybrid layers that protect the resin-dentin interface from harmful biological and chemical activities commonly occurring in the oral environment.

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“…Since the introduction of the adhesive systems, over 50 years ago, the union interface to dentin remains the weakest link in restorative treatment [1][2][3][4] . Although many studies have shown excellent short-term and immediate adhesion effectiveness 5,6 , the durability and stability of the adhesive interface on dentin remain questionable 7,8 due to its inherent characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…Although many studies have shown excellent short-term and immediate adhesion effectiveness 5,6 , the durability and stability of the adhesive interface on dentin remain questionable 7,8 due to its inherent characteristics. The failure of adhesion may lead marginal infiltration, which may cause discoloration, secondary caries, and subsequent loss of retention 3,9,10 . In order to minimize adhesive failures, universal adhesives have become a trend in dentistry due to its effectiveness and longevity, and a simplified operative technique.…”

Section: Introductionmentioning

confidence: 99%

Dentin bond strength evaluation between a conventional and universal adhesive using etch-and-rinse strategy

et al. 2021

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Aim: The aim of this study was to compare the microtensile bond strength (μTBS) and the characteristics of the adhesive interface of Scotchbond Universal - SU – etch-and-rise mode (3M ESPE) and Adper Scotchbond Multi-Purpose - MP (3M ESPE) to dentin over time. Methods: Class I cavity preparations were performed in 60 human molars that were randomly divided according to the dentin bonding system (DBS) used (n=30): (1) Acid conditioning + SU and (2) Acid conditioning + MP. For bonding strength (BS) analysis, 30 teeth (n = 15) were sectioned into sticks and submitted to the microtensile test in a universal testing machine after 24 hours and 12 months. The adhesive interface of the others 30 teeth was analyzed in a confocal microscope after 24 hours and 12 months. The data of μTBS were analyzed by two-way repeated measures ANOVA and Tukey’s HSD (α = 0.05). Results: SU presented the lowest DBS compared to MP (p=0.000). Time did not influenced DBS for both adhesive systems (p=0.177). Confocal microscopy analysis showed no cracks between both adhesive systems tested. Conclusion: The results indicate that MP - μTBS showed a better performance compared to SU in total-etch mode.

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Water-associated attributes in the contemporary dentin bonding milieu

Cited by 23 publications

References 53 publications

Chitosan-Based Extrafibrillar Demineralization for Dentin Bonding

Chitosan-Based Extrafibrillar Demineralization for Dentin Bonding

Evaluation of a Collagen-Reactive Monomer with Advanced Bonding Durability

Dentin bond strength evaluation between a conventional and universal adhesive using etch-and-rinse strategy

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