Water Channels and Zymogen Granules in Salivary Glands

Ishikawa, Yasuko; Cho, Gota; Yuan, Zhenfang; Skowroński, Mariusz T.; Pan, Yan; Ishida, Hideyuki

doi:10.1254/jphs.crj06007x

Cited by 52 publications

(38 citation statements)

References 157 publications

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“…AQPs play a pivotal role for normal gastrointestinal homeostasis, all from the secretion of saliva where AQP5 plays an important role [191][192][193], through the water-tight regulated stomach where AQP1, 3 and 4 play major roles in the secretion of gastric juices [189,[194][195][196] and the small intestine, where enterocytes immense bidirectional water transport where AQP1, 3, 7, 10 and 11 are expressed [189,195,197] to the ascending colon where primarily AQP1, 3, 7, 8 and 11 are present [198][199][200]. Several studies have displayed the importance of AQPs in faecal concentration/water absorption in the colon.…”

Section: Aquaporins In Gastrointestinal Homeostasis and Diarrhoeamentioning

confidence: 99%

Aquaporins in Infection and Inflammation

Holm¹

2016

Linköping University Medical Dissertations

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About the cover:The front cover displays a human, blood-derived macrophage stained for aquaporin 9.During the course of the research underlying this thesis, Angelika Holm was enrolled in Forum Scientium, a multidisciplinary doctoral programme at Linköping University Printed by LiU-Tryck, Linköping, Sweden, 2016 "Be brave, even if you're not, pretend to be. No one can tell the difference."To that young, awkward, tall girl who dared to dream of something else, something more:You did it. SUPERVISOR Elena VikströmDepartment of Clinical and Experimental Medicine Linköping University Linköping Sweden When cells of the innate immune system encounter pathogens they need to respond and prepare for migration and phagocytosis and do so through volume regulatory processes. The Gramnegative bacterium Pseudomonas aeruginosa utilizes a small molecule-based communication system, called quorum sensing (QS) to control the production of its virulence factors and biofilms. We found that P. aeruginosa with a complete QS system elicits a stronger phagocytic response in human blood-derived macrophages compared to its lasI-/rhlI-mutant lacking the production of the QS molecules N-butyryl-L-homoserine lactone (C4-HSL) and N-3-oxododecanoyl-L-homoserine lactone (3O-C12-HSL). Infection with P. aeruginosa further increases the expression of AQP9 and induces re-localisation of AQP9 to the front and trailing ends of macrophages. Moreover, the 3O-C12-HSL alone elevates the expression of AQP9, redistribute the water channel to the front and rear ends and increases the cell area and volume of macrophages. Both infection with the wild type P. aeruginosa and the treatment with 3O-C12-HSL change the nano-structural architecture of the AQP9 distribution in macrophages. ASSISTANT SUPERVISORS Karl-Eric MagnussonViruses use the intracellular machinery of the invaded cells to produce and assemble new viral bodies. Intracellular AQPs are localised in a membranes of cellular organelles to regulate their function and morphology. C3H10T1/2 fibroblasts transiently expressing green fluorescent protein (GFP)-AQP6 show a reduced expression of AQP6 after Hazara virus infection and an increased cell area. Overexpressing AQP6 in C3H10T1/2 cells reduces the infectivity of Hazara virus indicating that AQP6 expression has a protective role in virus infections.Ion and water channels in the epithelial cell lining tightly regulate the water homeostasis. In microscopic colitis (MC), patients suffer from severe watery diarrhoeas. For the first time, we have shown that the expression of AQP1, 8 and 11 and the sodium/hydrogen exchanger NHE1 are reduced in colonic biopsies from MC patients compared to healthy control individuals. Following treatment with the glucocorticoid budesonide the patients experienced a rapid recovery and we observed a restored or increased expression of the AQPs and NHE1 during treatment, suggesting a role for AQPs in the diarrhoeal mechanisms in MC.Taken together, this thesis provides new evidence on the importance of water homeostasis regulatio...

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Section: Aquaporins In Gastrointestinal Homeostasis and Diarrhoeamentioning

confidence: 99%

Aquaporins in Infection and Inflammation

Holm¹

2016

Linköping University Medical Dissertations

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“…conserved asparagine-proline-alanine (NPA) boxes consisting of AQP11 and AQP12 (reviewed by Ishikawa et al 2006).…”

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confidence: 99%

Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System

Skowroński

Kwon

Nielsen

2008

J Histochem Cytochem.

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(SN) S U M M A R Y Thirteen mammalian aquaporin (AQP) isoforms have been identified, and they have a unique tissue-specific pattern of expression. AQPs have been documented in the reproductive system of both male and female humans, rats, and mice. However, tissue expression and cellular and subcellular localization of AQPs are unknown in the female reproductive system of pigs. In this study, AQP1 immunoreactivity was detected in the capillary endothelium of the ovary. Distinct immunolabeling of capillary endothelium was also observed in the oviduct and uterus. AQP5 was expressed in flattened follicle cells of primordial follicles, granulosa cells of developing ovarian follicles, and muscle cells of the oviduct and uterus. Staining of AQP5 was also observed in the epithelial cells of the oviduct and uterine epithelium. AQP9 immunoreactivity was observed in granulosa cells of developing follicles. AQP9 was also localized in the luminal epithelial cells of the oviduct and uterine epithelia cells. This is, to our knowledge, the first study that shows tissue expression and cellular and subcellular localization of AQPs in the reproductive system of the female pig. Moreover, these results suggest that several subtypes of the AQPs (AQP1, 5, and 9) are involved in regulation of water homeostasis in the reproductive system of gilts. The first confirmation of AQP in the female reproductive system was obtained by isolating and sequencing the cDNA encoding a water channel from the human uterus (Li et al. 1994). Afterward, Li et al. (1997) found AQP1 mRNA in the rat uterus. To date, based on protein expression, at least nine AQP isoforms have been shown to be expressed in the female reproductive system of humans, rats, and mice (reviewed by Huang et al. 2006). Their specific expression pattern suggests that they play a role in water movement between the intraluminal, interstitial, and capillary compartments. There is also evidence indicating that ovarian steroid can regulate the expression of several AQPs (Jablonski et al. 2003;Branes et al. 2005;Lindsay and Murphy 2006). Nevertheless, data concerning the expression and role of AQPs in female reproductive tissues are still very limited and not available in relation to farm animals.

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“…Recently, their presence has been demonstrated in secretory granules and vesicles, indicating an involvement in volume regulation of these structures 26) . In the salivary glands, AQP5 is probably involved in providing for an appropriate amount of fluid to be secreted 26,27) . In particular, in rat salivary glands, AQP5 is highly expressed in the apical plasma membrane of serous acinar cells, in the secretory canaliculi and in the intercalated duct cells.…”

Section: Discussionmentioning

confidence: 99%

Immunohistological Study of the Major Salivary Glands in the Gray Short-Tailed Opossums (<i>Monodelphis domestica</i>)

Gotouda

Tamamura

Kôno

et al. 2017

J. Hard Tissue Biology.

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Marsupials were mammals whose young were born in an undeveloped stage and attached to the mother's nipples, usually in a pouch. Despite, these jaw elements to suckle and digestive system must develop early. Previously we have reported that the morphology of the salivary glands in the gray short-tailed opossums (Monodelphis domestica). This opossum, a kind of marsupial, has three large salivary glands which include the parotid, submandibular and sublingual glands. Parotid glands consist only of serous acinar cells. Submandibular and sublingual glands consist of seromucous secretory end pieces and the submandibular and sublingual glands were mixed glands. This study was purpose to investigate the localization of cytokeratin19 (CK19), aquaporin5 (AQP5) and alpha smooth muscle actin (SMA) in the gray short-tailed opossum parotid, submandibular and sublingual glands. These proteins localization was determined by immunohistochemistry on paraffin sections. Submandibular and sublingual glands showed a broadly similar staining pattern. CK19 was localized to the acinar cells and ductal cells in three major salivary glands. AQP5 was expressed in the luminal only of serous cells. Alpha-SMA was observed around the secretory end pieces. These results are thought to reflect the characteristic of the opossum salivary glands. Further studies are required to unravel obscure matters with cell differentiation and physiological functions of salivary glands among the mammals.

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Water Channels and Zymogen Granules in Salivary Glands

Cited by 52 publications

References 157 publications

Aquaporins in Infection and Inflammation

Aquaporins in Infection and Inflammation

Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System

Immunohistological Study of the Major Salivary Glands in the Gray Short-Tailed Opossums (<i>Monodelphis domestica</i>)

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