Water Elution Chromatography of Amino Acids on Ion Exchange Materials

Abstract

Successful chromatography of amino acids has been performed on the free acid and base forms of a number of

Automatic Ion Exclusion-Partition Chromatography of Acids.

“…Because paper chromatographic methods have been used to identify phenols in many types of mixtures, it was considered worth while to attempt a study of phenols in automobile exhaust by this procedure. Of the various procedures tried, the most satisfactory was that of Crump (2), which uses the o-and p-nitro azo derivatives of phenols for paper chromatography.…”

“…Procedure. Crump's system (2) of benzene-cyclohexane-dipropylene glycol (in the ratio of 30:70:3, v./v.) with papers impregnated with 20% formamide was used for separating phenols in which the 4 position is unsubstituted.…”

Analysis of Mixtures of Sugars and Mixtures of Amino Acids by Dialysis Based on Differential Kinetics.

“…Mixtures of amino acids are separated by paper and column chromatography (8) and ion exchange chromatography (3,8,13,14)• Mixtures of amino acids that yield volatile aldehydes can be oxidized and separated in a gas chromatography unit (15). Gas chromatography has been used also to separate derivatives of amino acids (6, 11, 16).…”

The Role of Separations in Organic Analysis

“…Choline, betaine, and other natural occurring nitrogenous compounds were separated on Dowex 50 columns before eventual colorimetric determination (12). Ion exchange materials were used as a practical means of separating amino acids and similar materials (10). A mixed bed of cation and anion exchangers was used to remove interfering substances in the determination of betaine in sugar-beet juices and plant material (15).…”

“…The calculated titration curve agrees fairly well with the experimental titration curve. Functional group approaches to the determination of aromatic hydrocarbons have been limited to such methods as base-catalyzed condensation at a methylene carbon as for cyclopentadiene or indene ( 16), and Diels-Alder reactions as for anthracene (10,15,17). General colorimetric determinations of aromatics have been limited to nitration (9) and the recent phosphorus pentachloride-catalyzed condensation of piperonal (14) with aromatics more basic than toluene.…”