Water of Increased Content of Molecular Oxygen

Chwastowski, Jarosław; Ciesielski, Wojciech; Khachatryan, Karen; Kołoczek, Henryk; Kulawik, Damian; Oszczęda, Zdzisław; Soroka, Jacek A.; Tomasik, Piotr; Witczak, Mariusz

doi:10.3390/w12092488

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…A large amount of evidence has been collected, suggesting that mechanical stress can affect the properties of biomolecules and cells (Dotsenko & Mischenko, 2011;Dotsenko et al, 2014;Bunkin et al, 2020). Erythrocytes exposed to vibration were kept in buffer solution saturated with O 2 (Chwastowski et al, 2020). O 2 itself, along with other gases, is found in bubbles with the diameter of less than a micron -nanobubbles (Bunkin et al, 2011;Shatalov et al, 2012;Li & Bunkin, 2019).…”

Section: Discussionmentioning

confidence: 99%

Vibration influence on the O2-dependent processes activity in human erythrocytes

Dotsenko

Mischenko

Taradina

2021

Regul. Mech. Biosyst.

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The early signs of vibration effects on the human body are microcirculation and transcapillary metabolism disorders, accompanied by disruption of the supply to and utilization of oxygen in the tissues and organs. However, there are few experimental studies aimed at finding targets of vibration in cells and determining the action mechanism of vibration. In in vitro experiments, human erythrocytes in buffer solution were exposed to low-frequency vibration (frequency range 8–32 Hz, amplitudes 0.5–0.9 mm) for 3 hours. The dynamics of the accumulation of membrane-bound catalase and hemoglobin and the distribution of ligand hemoglobin in the membrane-bound fraction were studied as the indicators of functional activity of cells. The choice of these indicators is justified by the participation of catalase and hemoglobin in O2-dependent cellular reactions as a part of protein complexes. Since pО2 is a trigger of conformational transitions in the hemoglobin molecule, simultaneously with oxygen transport, hemoglobin signals to different metabolic systems about oxygen conditions in the environment. The studies revealed that in the conditions of vibration, the activity of membrane-associated catalase increased by 40–50% in the frequency range of 12–24 Hz (amplitude 0.5 ± 0.04 mm), by 20–30% in the amplitude of 0.9 mm, but after about 100–120 min exposure the enzyme activity decreased even below the control level. There was a dose-dependent accumulation of membrane-bound hemoglobin during exposure to vibration. In the membrane-bound fraction of hemoglobin, oxyhemoglobin had the highest content (60–80%), while the content of methemoglobin varied 5–20%. During vibrations in the frequency range 12–28 Hz, 0.5 mm, we recorded 10–30% increase in oxyhemoglobin. With increase in the vibration amplitude (0.9 mm) in the frequency range of 16–32 Hz, constant content of oxyhemoglobin was noted at the beginning of the experiment, which tended to decrease during the last exposure time. Frequency of 32 Hz caused increase in the deoxyhemoglobin content in the membrane-bound fraction. The content of methemoglobin (metHb) in erythrocytes significantly increased during exposure to the frequency range of 12–24 Hz, with the amplitude of 0.5 mm (1.3–2.4 times). During the exposure to frequencies of 28 and 32 Hz, we observed the transition of methemoglobin to hemichrome. The content of methemoglobin in the cells was lower and decreased at the end of the experiment when the vibration amplitude was 0.9 mm. In these experimental conditions, no increase in hemichrome content in the membrane-bound fraction was recorded. Therefore, the degree of binding of catalase and hemoglobin with the membrane of erythrocytes that were exposed to vibration and the changes in the content of ligand forms in the composition of membrane-bound hemoglobin are dose-dependent. Low-frequency vibration initiates O2-dependent processes in erythrocytes. Targets of such an influence are nanobubbles of dissolved air (babstons), retained on the surface of erythrocytes due to Coulomb interactions, capable of coagulation and increase in size under the action of vibration. At first, the consequences of these processes are increase in oxygen content in the surface of erythrocytes, and then decrease as a result of degassing. Thus, increase in oxygen content on the surface initiates redox reactions, whereas decrease in oxygen content leads to reconstruction of metabolic processes oriented at overcoming hypoxia.

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Section: Discussionmentioning

confidence: 99%

Vibration influence on the O2-dependent processes activity in human erythrocytes

Dotsenko

Mischenko

Taradina

2021

Regul. Mech. Biosyst.

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“…Water treated for the same time with LGPG under nitrogen as described by Chwastowski et al, [7] produced LPGPN. LPGPC [13], LPGPO [9] and LPGPM [12] were prepared following the procedures described in the indicated references. LPGP of 38 • C was generated at 5 × 10 −3 mbar, 800 V, 50 mA and 10 KHz frequency in a plasmothrone patented by Oszczęda et al [1,10].…”

Section: Watermentioning

confidence: 99%

“…LPGP was found to be different to other reported kinds of plasma [2][3][4][5][6] (see also the relevant literature survey in our recent paper [7]) because it does not break valence bonds; therefore, treatment with LPGP does not evoke any chemical reaction. Thus, single oxygen atoms, hydrogen peroxide and ozone cannot be formed when water is treated with LPGP in the air [8], as well as under molecular oxygen [9].…”

Section: Introductionmentioning

confidence: 99%

“…Following the invention of LPGP and the construction of a generator suitable for the treatment of water [1,7,10], a series of papers were published on the specific physical and physicochemical properties of water treated with LPGP in the air (LPGPA) [8], under all oxygen-free nitrogen (LPGPN) [7], ammonia (LPGPAM) [11], methane (LPGPM) [12], carbon dioxide (LPGPC) [13], and molecular oxygen (LPGPO) [9]. Based on these various studies, the functional properties of such treated water could be applied to agriculture and various branches of industry, as well as human and animal prophylaxis and therapy.…”

Section: Introductionmentioning

confidence: 99%

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Reaction of Lavandula angustifolia Mill. to Water Treated with Low-Temperature, Low-Pressure Glow Plasma of Low Frequency

Ciesielska¹,

Ciesielski²,

Girek³

et al. 2020

Water

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Lavandula angustifolia was watered with either deionized tap water treated with low-temperature, low-pressure glow plasma of low frequency in the air (LPGPA), under oxygen-free nitrogen (LPGPN), methane (LPGPM), carbon dioxide (LPGPC) or molecular oxygen (LPGPO). The crop yields were slightly dependent on the type of water used for watering. Notably, only plants watered with LPGPN showed a slightly higher crop yield. The plants also contained a higher level of protein and bioaccumulated magnesium. The type of water had a considerable and specific effect on the yield of isolated essential oils and their composition. The yield of essential oil decreased in the following order LPGPA = LPGPN (0.4 g/100 g dry mass) > LPGPC = LPGPO (0.3 g/100 g dry mass) > LPGPM = non-treated water (0.2 g/100 g dry mass). The composition of the isolated essential oils varied depending on the type of water used for watering, which influences their role as a fragrant component of cosmetics, and in herbal therapy and aromatherapy.

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“…Following the invention of low-temperature, low-pressure glow plasma of low frequency (LPGP) and the constructing of generators for such plasma [1,2], a series of papers was published describing the macrostructure and the resulting physical, physicochemical and chemical properties of water treated with LPGP. It appeared that these properties depended, among other things, on whether that treatment was performed in the air, under nitrogen, ammonia, carbon dioxide, methane or molecular oxygen [3][4][5][6][7][8]. Subsequent papers demonstrated that these diverse properties resulted in specific functional properties for different kinds of water.…”

Section: Introductionmentioning

confidence: 99%

Effect of Watering of Selected Seasoning Herbs with Water Treated with Low-Temperature, Low-Pressure Glow Plasma of Low Frequency

Ciesielska

Ciesielski

Girek

et al. 2020

Water

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Plantations of lovage, marjoram, rosemary and thyme were watered with water treated with low-temperature, low-pressure glow plasma of low frequency. Such watering appeared beneficial to the extent dependent on particular herb. In terms of crop yield and quality, water treated with glow plasma performed best in the case of rosemary, and the worst results were observed for thyme. When yield of essential oils are taken into account, only in the case of lovage did such watering appear beneficial, while in the remaining cases it had no effect. However, such watering considerably changed the composition of essential oils. These changes were specific for a given herb and involved the quantity of particular components of the oils. Only in the essential oil from lovage did γ-terpinene appear as its novel component.

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Water of Increased Content of Molecular Oxygen

Cited by 9 publications

References 21 publications

Vibration influence on the O2-dependent processes activity in human erythrocytes

Vibration influence on the O2-dependent processes activity in human erythrocytes

Reaction of Lavandula angustifolia Mill. to Water Treated with Low-Temperature, Low-Pressure Glow Plasma of Low Frequency

Effect of Watering of Selected Seasoning Herbs with Water Treated with Low-Temperature, Low-Pressure Glow Plasma of Low Frequency

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