Water Quality

Gerba, Charles P.; Rock, Channah

doi:10.1016/b978-0-12-404611-5.00006-3

Cited by 6 publications

(1 citation statement)

References 65 publications

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“…Unfortunately, this eliminates the possibility of quantification because recovery rates are highly variable, and dependent on the organism. Still, enrichment is the basis of several procedures that have successfully produced incidence data on water samples (Lynch et al ., ; Cooley et al ., ; Gorski et al ., ; Strawn et al ., ; Walters et al ., ; Cooley et al ., ; Gerba and Rock, ; Strawn et al ., ; Jokinen et al ., ; Weller et al ., ). Despite the lack of quantification, the sensitivity of these enrichment methods is excellent (generally less than 10 CFU/100 ml).…”

Section: Introductionmentioning

confidence: 99%

New strategies for the enumeration of enteric pathogens in water

Gorski

Rivadeneira

Cooley

2019

Environmental Microbiology Reports

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Water quality standards for drinking water and recreational waters have long been based on the enumeration of faecal coliforms in the various water supplies, with 0 CFU Escherichia coli/100 ml for drinking water and <126 CFU generic E. coli/100 ml for recreational waters. Irrigation water will soon undergo the same scrutiny in the United States. For over 50 years the most probable number method has been used by laboratories to estimate the level of viable bacteria in a sample, but this method is labour intensive and slow, especially if large numbers of samples need to be tested. In this review, we describe some recent innovations in methods to enumerate enteric pathogens in water. These methods are based on different reasoning schemes that can be categorized as biosensors and nucleic acid-based methods. All the methods described here used natural water sources. Several were also used to survey the bacterial levels in naturally contaminated samples. The different methods vary in their limits of detection, ease of use, and potential portability. Some combine very good limits of detection with the ability to overcome technical challenges; however, there is considerable room for improvement, as none of the methods are without shortcomings.

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