Water Relations, Gas Exchange, Chlorophyll Fluorescence and Electrolyte Leakage of Ectomycorrhizal Pinus halepensis Seedlings in Response to Multi-Heavy Metal Stresses (Pb, Zn, Cd)

Hachani, Chadlia; Lamhamedi, Mohammed S.; Abidine, Abdenbi Zine El; Abassi, Mejda; Khasa, Damase; Béjaoui, Zoubeir

doi:10.3390/microorganisms10010057

Cited by 8 publications

(11 citation statements)

References 84 publications

(141 reference statements)

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“…The negative effect of heat stress on the physiological activity of many tree species is well documented [ 48 ], indicating that such perturbations can either result in reduced tree growth and production [ 49 ] or increased tree mortality [ 50 ]. To date, there is a lot of evidence concerning the positive effect of ectomycorrhizal fungi on plants’ gas exchange when exposed to different stress factors, including water scarcity [ 51 ], heavy metals [ 52 ], and salinity [ 53 ]; however, little research has attempted to answer the question of how ectomycorrhizal fungi can alleviate the negative effect of heat stress on plants through the modulation of gas exchange parameters. Indeed, most studies have focused on the role of arbuscular mycorrhizal fungi in the physiological response of plants to heat-induced stress [ 54 , 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Ectomycorrhizal Fungi Modulate Pedunculate Oak’s Heat Stress Responses through the Alternation of Polyamines, Phenolics, and Osmotica Content

Kebert

Kostić

Čapelja

et al. 2022

Plants

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The physiological and biochemical responses of pedunculate oaks (Quercus robur L.) to heat stress (HS) and mycorrhization (individually as well in combination) were estimated. One-year-old Q. robur seedlings were grown under controlled conditions in a pot experiment, inoculated with a commercial inoculum of ectomycorrhizal (ECM) fungi, and subjected to 72 h of heat stress (40 °C/30 °C day/night temperature, relative humidity 80%, photoperiod 16/8 h) in a climate chamber, and they were compared with seedlings that were grown at room temperature (RT). An in-depth analysis of certain well-known stress-related metrics such as proline, total phenolics, FRAP, ABTS, non-protein thiols, and lipid peroxidation revealed that mycorrhized oak seedlings were more resistant to heat stress (HS) than non-mycorrhized oaks. Additionally, levels of specific polyamines, total phenolics, flavonoids, and condensed tannins as well as osmotica (proline and glycine betaine) content were measured and compared between four treatments: plants inoculated with ectomycorrhizal fungi exposed to heat stress (ECM-HS) and those grown only at RT (ECM-RT) versus non-mycorrhized controls exposed to heat stress (NM-HS) and those grown only at room temperature (NM-RT). In ectomycorrhiza inoculated oak seedlings, heat stress led to not only a rise in proline, total phenols, FRAP, ABTS, non-protein thiols, and lipid peroxidation but a notable decrease in glycine betaine and flavonoids. Amounts of three main polyamines (putrescine, spermine, and spermidine) were quantified by using high-performance liquid chromatography coupled with fluorescent detection (HPLC/FLD) after derivatization with dansyl-chloride. Heat stress significantly increased putrescine levels in non-mycorrhized oak seedlings but had no effect on spermidine or spermine levels, whereas heat stress significantly increased all inspected polyamine levels in oak seedlings inoculated with ectomycorrhizal inoculum. Spermidine (SPD) and spermine (SPM) contents were significantly higher in ECM-inoculated plants during heat stress (approximately 940 and 630 nmol g−1 DW, respectively), whereas these compounds were present in smaller amounts in non-mycorrhized oak seedlings (between 510 and 550 nmol g−1 DW for Spd and between 350 and 450 nmol g−1 DW for Spm). These findings supported the priming and biofertilizer roles of ectomycorrhizal fungi in the mitigation of heat stress in pedunculate oaks by modification of polyamines, phenolics, and osmotica content.

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Section: Discussionmentioning

confidence: 99%

Ectomycorrhizal Fungi Modulate Pedunculate Oak’s Heat Stress Responses through the Alternation of Polyamines, Phenolics, and Osmotica Content

Kebert

Kostić

Čapelja

et al. 2022

Plants

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“…The increased leaf ELR reflected a disrupted plasma membrane permeability [ 45 ]. It was reported that heavy metal toxicity such as excessive Pb, Zn and Cd significantly increased the ELR in needles of Pinus halepensis [ 46 ]. Moreover, it was also identified that Cu-sensitive jute varieties had significantly higher ELR than the Cu-resistant ones [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Characteristics and Chloroplast Ultrastructure Responses of Citrus Leaves to Copper Toxicity Induced by Bordeaux Mixture in Greenhouse

Lu¹,

Hu²,

Lin³

et al. 2022

IJMS

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Bordeaux mixture (Bm) is a copper (Cu)-based pesticide that has been widely used for controlling citrus scab and citrus canker. However, frequent spraying of Bm is toxic to citrus. To our knowledge, few studies are available that discuss how the photosynthetic characteristics and chloroplast ultrastructure of citrus leaves are affected by Cu toxicity induced by excessive Bm. In the study, two-year-old seedlings of Citrus grandis (C. grandis) and Citrus sinensis (C. sinensis), which were precultured in pots, were foliar-sprayed with deionized water (as control) or Bm diluted 500-fold at intervals of 7 days for 6 times (4 times as recommended by the manufacturer) to investigate the leaf Cu absorption, photosynthesis, chloroplast ultrastructure and antioxidant enzymatic activities. Bm foliar-sprayed 6 times on citrus seedlings increased the leaf Cu content, decreased the photosynthetic pigments content and destroyed the chloroplast ultrastructure, which induced leaf chlorosis and photosynthetic inhibition. A lower Cu absorption, a higher light photon-electron transfer efficiency, a relative integrity of chloroplast ultrastructure and a promoted antioxidant protection contributed to a higher photosynthetic activity of C. grandis than C. sinensis under excessive spraying of Bm. The present study provides crucial references for screening and selecting citrus species with a higher tolerance to Cu toxicity induced by excessive Bm.

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“…Pukacki Odasz-Albrigtsen et al, 2000;Alessio et al, 2002;Naidoo and Chirkoot, 2004;Matsushima et al, 2009;Fusaro et al, 2021Wang et al, 2019 Pinus pinea, Quercus ilex; Hibiscus sp. ; Avicennia marina; Betula pubescens, Pinus sylvestris and 5 shrub species; Morus alba Heavy metals Kitao et al, 1998;Pereira et al, 2000;Dezhban et al, 2015;Zhang et al, 2020;Hachani et al, 2021;Reyes et al, 2022 Robinia pseudoacacia; Pinus halepensis; Betula ermanii, Alnus hirsuta; 4 Citrus species/cultivars; Quercus ilex, Nerium oleander, Pittosporum tobira; Citrus grandis, Citrus sinensis…”

Section: Gaseous Air Pollutantsmentioning

confidence: 99%

“…On the other hand, the ChF method allowed to confirm the beneficial effect of ectomycorrhizal fungi on recovery from contamination stress (Pb, ZN and Cd) in Pinus halepensis Mill. ( Hachani et al., 2021 ). The research on soil pollution influence was conducted mostly on crop plants.…”

Section: Chlorophyll a Fluorescence Measurements I...mentioning

confidence: 99%

Environmental stress - what can we learn from chlorophyll a fluorescence analysis in woody plants? A review

et al. 2022

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Chlorophyll a fluorescence (ChF) signal analysis has become a widely used and rapid, non-invasive technique to study the photosynthetic process under stress conditions. It monitors plant responses to various environmental factors affecting plants under experimental and field conditions. Thus, it enables extensive research in ecology and benefits forestry, agriculture, horticulture, and arboriculture. Woody plants, especially trees, as organisms with a considerable life span, have a different life strategy than herbaceous plants and show more complex responses to stress. The range of changes in photosynthetic efficiency of trees depends on their age, ontogeny, species-specific characteristics, and acclimation ability. This review compiles the results of the most commonly used ChF techniques at the foliar scale. We describe the results of experimental studies to identify stress factors that affect photosynthetic efficiency and analyse the experience of assessing tree vigour in natural and human-modified environments. We discuss both the circumstances under which ChF can be successfully used to assess woody plant health and the ChF parameters that can be useful in field research. Finally, we summarise the advantages and limitations of the ChF method in research on trees, shrubs, and woody vines.

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Water Relations, Gas Exchange, Chlorophyll Fluorescence and Electrolyte Leakage of Ectomycorrhizal Pinus halepensis Seedlings in Response to Multi-Heavy Metal Stresses (Pb, Zn, Cd)

Cited by 8 publications

References 84 publications

Ectomycorrhizal Fungi Modulate Pedunculate Oak’s Heat Stress Responses through the Alternation of Polyamines, Phenolics, and Osmotica Content

Ectomycorrhizal Fungi Modulate Pedunculate Oak’s Heat Stress Responses through the Alternation of Polyamines, Phenolics, and Osmotica Content

Photosynthetic Characteristics and Chloroplast Ultrastructure Responses of Citrus Leaves to Copper Toxicity Induced by Bordeaux Mixture in Greenhouse

Environmental stress - what can we learn from chlorophyll a fluorescence analysis in woody plants? A review

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