In the water production industry, reverse osmosis (RO) membrane technology is a durable, promising, and much-used separation method. Its application enables the efficient removal of a wide variety of contaminants (i.e., microbial constituents, total dissolved solids, and organic compounds). Feed streams of different qualities (e.g., raw, natural, chemically contaminated or brackish, and seawater) are used to produce high-purity water that is microbiologically safe and biologically stable (15,25). However, the widespread application of this technology is limited because the current generation of RO filtration units experience biofouling problems (14). The design of so-called "spiral wound" membrane elements and the conditions at the membrane, feed-side spacer, and other internal surfaces within these RO filters make them prone to microbial attachment and the subsequent formation of biofilm layers. A variety of microorganisms are involved in the development of these surface-attached complex structures after prolonged operation of the RO system, depending on the type and concentration of contaminants in the feed water and the type of pretreatment (5,6,7,32,38). The biofilm occurrence is a principal problem for proper RO system performance. It can lead to blocking of the feed concentrate channel and to clogging of the membrane. Biofilm formation results in an increased energy requirement of the feed water pumps, a lower flux, and a decrease of permeate quality