Water‐Soluble Fluorescent Probes Based on Dendronized Polyfluorenes for Cell Imaging

Bai, Libin; Li, Wei; Chen, Jingtian; Bo, Fuli; Gao, Baoxiang; Liu, Hongmei; Li, Jingjing; Wu, Yonggang; Ba, Xinwu

doi:10.1002/marc.201200722

Cited by 16 publications

(5 citation statements)

References 60 publications

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“…Fluorescent materials have broad applications in biosensors, cellular imaging, drug delivery, and so on. The most commonly studied fluorescent materials include fluorescent proteins (especially green fluorescent proteins), quantum dots (QDs), and fluorescent organic dyes .…”

Section: Introductionmentioning

confidence: 99%

Strong Fluorescence of Poly(N-vinylpyrrolidone) and Its Oxidized Hydrolyzate

Song

Lin

Zhu

et al. 2014

Macromol. Rapid Commun.

129

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Discovering fluorescence of existing compounds, which are generally regarded as non-fluorescent, is of important academic and technical significance. This article reports the fluorescence of common compounds containing pyrrolidone ring(s) and their oxidized hydrolyzates. Poly(N-vinylpyrrolidone) (PVP), polymerized from a very weak fluorescent monomer N-vinyl-2-pyrrolidone (NVP), exhibits strong intrinsic fluorescence. Moreover, the fluorescence of its "hydrolyzate" is dramatically enhanced by about 1000 times. The "hydrolyzate" of N-methyl-pyrrolidone (NMP) also exhibits significantly enhanced fluorescence. By studying the chemical structures and fluorescence of the hydrolyzates, the enhanced fluorescence is attributed to the formation of secondary amine oxide. The much stronger fluorescence of the polymers compared to the corresponding small molecular compounds is ascribed to the "aggregation-induced emission" (AIE) effect of the luminophores. PVP and its oxidized hydrolyzate also show some phenomena different to the common AIE effect. The fluorescence of PVP and its oxidized hydrolyzate shows stimuli response to metal ions and pH values. This study introduces novel fluorescent materials for various potential applications.

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Section: Introductionmentioning

confidence: 99%

Strong Fluorescence of Poly(N-vinylpyrrolidone) and Its Oxidized Hydrolyzate

Song

Lin

Zhu

et al. 2014

Macromol. Rapid Commun.

129

View full text Add to dashboard Cite

show abstract

“…However, the control one showed no effective fluorescence. Until now, widely used probes with low fluorescence quantum yields often resulted in a high dose usage to achieve better imaging effect, generally above 5 μM, which was accompanied by increasing cytotoxicity. , In this work, due to the high Φ value of Per-6-Diimi[Gd(NO 3 ) 4 ] in the hydrophobic environment, the imaging concentration of fluorescent molecules was much decreased.…”

Section: Results and Discussionmentioning

confidence: 83%

Dual-Mode Fluorescence and Magnetic Resonance Imaging by Perylene Diimide-Based Gd-Containing Magnetic Ionic Liquids

Yuan

Huang

et al. 2020

ACS Biomater. Sci. Eng.

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Bioimaging plays a key role in the diagnosis/treatment of diseases and in scientific research studies. Compared with single imaging techniques, dual-mode and multimode imaging techniques facilitate high accuracy. In this work, a perylene diimide (PDI)-based Gd-containing magnetic ionic liquid, Per-6-Diimi[Gd(NO3)4], is reported for dual-modal imaging, in which a Gd(III) complex was used for magnetic resonance imaging (MRI), while PDI was used for fluorescence imaging. Because of the difference in the biological microenvironment, there is a switch between dispersed and aggregated states of Per-6-Diimi[Gd(NO3)4] molecules in hydrophobic and hydrophilic media. When it was in the aqueous solution, the intensive π–π interaction of PDI cores made Per-6-Diimi[Gd(NO3)4] aggregates to form particles. The paramagnetic nanoparticles ensure prolonging the rotational correlation time, which results in a strong enhancement of MRI with a longitude relaxation coefficient of 14.94 mM–1 s–1. In an in vivo MRI experiment, the tumor site is imaged by MRI through the enhanced permeability and retention effect. However, when the molecule is present on the hydrophobic membrane of the cells, the dispersed Per-6-Diimi[Gd(NO3)4] showed good fluorescence imaging capabilities due to the high fluorescence quantum yield of PDI. Thus, the fluorescence imaging of cells can be carried out. Moreover, ex vivo fluorescence imaging of organs is performed after MRI. Per-6-Diimi[Gd(NO3)4] is enriched in the liver, kidneys, and tumors.

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“…However, the emission spectrum of FOP in water was significantly different to that in the organic solvents, with two less-intense peaks at 437 and 476 nm and considerably lower fluorescence intensity and QY. This is because the hydrophobic main chain of FOP causes it to aggregate in polar solutions, resulting in a lower fluorescence intensity and QY (Bai et al 2013). 5 Fluorescence emissions are prone to self-absorption and quenching when greater spectral overlap occurs between the absorption and emission spectra, resulting in a small Stokes shift (Zhou et al 2019).…”

Section: Spectroscopic Properties Of Fopmentioning

confidence: 99%

Selective and rapid detection of minocycline in milk using a novel fluorescence conjugated polymer probe

Gong,

Zhang,

Wang

et al. 2024

Preprint

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Minocycline is a broad-spectrum antibiotic widely used in clinical and veterinary medicine and its adverse effects are as prominent as its potent efficacy. To avoid its abuse and accumulation, it is very important to detect minocycline in medical science, food industry, and aquaculture industry. Here, a conjugated polymer probe for minocycline is prepared and its optical properties and fluorescence response to minocycline is studied. The quenching efficiency is linear relevant with the amount of minocycline at the concentration range of 0–12 µM in PBS and 0–28 µM in ethanol with the limit of detection of 0.0151 µM. Moreover, the standard addition recoveries of minocycline in skim milk samples are between 92.22–104.43%, and the relative standard deviation values are less than 3%. These results imply that the probe has a great potential for detecting minocycline in actual samples with simplify, rapidity and sensitivity.

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Water‐Soluble Fluorescent Probes Based on Dendronized Polyfluorenes for Cell Imaging

Cited by 16 publications

References 60 publications

Strong Fluorescence of Poly(N-vinylpyrrolidone) and Its Oxidized Hydrolyzate

Strong Fluorescence of Poly(N-vinylpyrrolidone) and Its Oxidized Hydrolyzate

Dual-Mode Fluorescence and Magnetic Resonance Imaging by Perylene Diimide-Based Gd-Containing Magnetic Ionic Liquids

Selective and rapid detection of minocycline in milk using a novel fluorescence conjugated polymer probe

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