Water-Soluble Lysine-containing Polypeptides. II. The Interaction of Several Sequential Lysine–Glycine Polypeptides with DNA. A Circular Dichroism Study of DNA Conformation in Annealed Complexes

Williams, Robert; Kielland, Sandra L.

doi:10.1139/v75-074

Cited by 10 publications

(7 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The CD spectra of the random (LAG)"-DNA complex, at various r values, are shown in Figure 1. These CD spectra are similar to those spectra observed for other systems: complexes of DNA and block (LysA)"(Leu3')m copolymers (Ong and Fasman, 1976), complexes of DNA and sequential (L-Alat.-Lys-i.-Pro)" (Sponar et al, 1973(Sponar et al, , 1975, complexes between DNA and histone HI (Fasman et al, 1970), complexes of poly(i.-lysine) and DNA (Williams and Kielland, 1975;Mandel and Fasman, 1976), and DNA in the presence of NaCl plus either polyethylene oxide or polyacrylate (Jordan et al, 1972). In addition, these ' DNA like spectra (Jordan et al, 1972) are also observed with complexes of DNA and copolymers of L-lysine and L-alanine (Stokrova et al, 1975) and with copolymers of L-lysine and glycine (Williams and Kielland, 1975).…”

Section: Discussionsupporting

confidence: 81%

Interaction of DNA with poly(L-Lys-L-Ala-Gly) and poly(L-Lys-L-Ala-L-Pro). Circular dichroism and thermal denaturation studies

Schwartz¹,

Fasman²

1977

Biochemistry

View full text Add to dashboard Cite

Complexes of DNA with polypeptides composed of Lys, Ala, and Gly in both a sequential order, poly(L-lysine-L-alanine-glycine), and a statistical distribution, poly(L-lysine36-L-alanine28-glycine), were prepared using gradient dialysis. These polypeptide-DNA complexes were studied using ultraviolet absorption (UV) and circular dichroism (CD) to probe the conformation, binding, and melting behavior of DNA in the complex. Complexes with the sequential polypeptide showed no structural change in the DNA; however, the complexes with the random polypeptide yield CD spectra similar to phi DNA [Maniatis, T., Venable, Jr., J.S., and Lerman, L.S. (1974), J. Mol. Biol. 84, 37]. A second sequential polypeptide, poly(L-Lys-L-Ala-L-Pro)n, -DNA complex was also studied. It was found to exhibit pronounced structural changes as a function of ionic strength and poly-peptide-DNA ratio, more similar to the random sequence that the ordered sequence of the Lys, Ala, Gly polymer. Thus the importance of the composition and amino acid sequence in polypeptides which bind to DNA, even in such simple systems, is demonstrated. Evidence from thermal denaturation, employing simultaneous monitoring of CD and UV changes, supports a model in which specific polypeptides cause condensation of the DNA in the complex into an asymmetric tertiary structure. The relevance of these model systems to chromatin is discussed.

show abstract

Section: Discussionsupporting

confidence: 81%

Interaction of DNA with poly(L-Lys-L-Ala-Gly) and poly(L-Lys-L-Ala-L-Pro). Circular dichroism and thermal denaturation studies

Schwartz¹,

Fasman²

1977

Biochemistry

View full text Add to dashboard Cite

show abstract

“…ct-DNA exhibits two characteristics peaks (Figure S4 in SI); one positive band around 280 nm complemented to π-π base stacking and a negative band around 245 nm for hellicity4243. While titrated with EB, DNA generates an induced CD signal near 303 nm (Figure S4) along with decreasing π-π stacking peak and increasing helicity peak, which is a well known feature for the intercalation of dye in DNA4445.…”

Section: Resultsmentioning

confidence: 98%

A Green Solvent Induced DNA Package

Satpathi

Sengupta

Hridya

et al. 2015

Sci Rep

View full text Add to dashboard Cite

Mechanistic details of DNA compaction is essential blue print for gene regulation in living organisms. Many in vitro studies have been implemented using several compaction agents. However, these compacting agents may have some kinds of cytotoxic effects to the cells. To minimize this aspect, several research works had been performed, but people have never focused green solvent, i.e. room temperature ionic liquid as DNA compaction agent. To the best of our knowledge, this is the first ever report where we have shown that guanidinium tris(pentafluoroethyl)trifluorophosphate (Gua-IL) acts as a DNA compacting agent. The compaction ability of Gua-IL has been verified by different spectroscopic techniques, like steady state emission, circular dichroism, dynamic light scattering and UV melting. Notably, we have extensively probed this compaction by Gua-IL through field emission scanning electron microscopy (FE-SEM) and fluorescence microscopy images. We also have discussed the plausible compaction mechanism process of DNA by Gua-IL. Our results suggest that Gua-IL forms a micellar kind of self aggregation above a certain concentration (≥1 mM), which instigates this compaction process. This study divulges the specific details of DNA compaction mechanism by a new class of compaction agent, which is highly biodegradable and eco friendly in nature.

show abstract

“…Circular dichroism spectroscopic measurements allow to gather information on the DNA secondary structure ( Satpathi et al, 2015 ), being commonly used to monitor nucleic acids structure perturbations ( Tateishi-Karimata and Sugimoto, 2014 ). The dsDNA from salmon testes, used in this work, presents a β-form conformation, exhibiting two characteristic peaks: one positive band at approximately 275–280 nm associated to π–π base stacking and a negative band around 245 nm corresponding to helicity ( Williams and Kielland, 1975 ; Evdokimov et al, 1976 ).…”

Section: Resultsmentioning

confidence: 99%

Insights on the DNA Stability in Aqueous Solutions of Ionic Liquids

Dinis

Sousa

Freire

2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Deoxyribonucleic acid (DNA) carries the genetic information essential for the growth and functioning of living organisms, playing a significant role in life sciences research. However, the long-term storage and preservation of DNA, while ensuring its bioactivity, are still current challenges to overcome. In this work, aqueous solutions of ionic liquids (ILs) were investigated as potential preservation media for double stranded (dsDNA). A screening of several ILs, by combining the cholinium, tetrabutylammonium, tetrabutylphosphonium, and 1-ethyl-3-methylimidazolium, cations with the anions bromide, chloride, dihydrogen phosphate, acetate, and glycolate, was carried out in order to gather fundamental knowledge on the molecular features of ILs that improve the dsDNA stability. Different IL concentrations and the pH effect were also addressed. Circular dichroism (CD) spectroscopy was used to evaluate the conformational structure and stability of dsDNA. IL-DNA interactions were appraised by UV-Vis absorption spectrophotometry and 31 P nuclear magnetic resonance (NMR) spectroscopy. The results obtained demonstrate that pH has a significant effect towards the dsDNA stability. Amongst the ILs investigated, cholinium-based ILs are the most promising class of ILs to preserve the dsDNA structure, in which electrostatic interactions between the cholinium cation and the DNA phosphate groups play a significant role as demonstrated by the 31 P NMR data, being more relevant at higher IL concentrations. On the other hand, the denaturation of dsDNA mainly occurs with ILs composed of more hydrophobic cations and able to establish dispersive interactions with the nucleobases environment. Furthermore, the IL anion has a weaker impact when compared to the IL cation effect to interact with DNA molecules. The experimental data of this work provide relevant fundamental knowledge for the application of ILs in the preservation of nucleic acids, being of high relevance in the biotechnology field.

show abstract

Water-Soluble Lysine-containing Polypeptides. II. The Interaction of Several Sequential Lysine–Glycine Polypeptides with DNA. A Circular Dichroism Study of DNA Conformation in Annealed Complexes

Cited by 10 publications

References 25 publications

Interaction of DNA with poly(L-Lys-L-Ala-Gly) and poly(L-Lys-L-Ala-L-Pro). Circular dichroism and thermal denaturation studies

Interaction of DNA with poly(L-Lys-L-Ala-Gly) and poly(L-Lys-L-Ala-L-Pro). Circular dichroism and thermal denaturation studies

A Green Solvent Induced DNA Package

Insights on the DNA Stability in Aqueous Solutions of Ionic Liquids

Contact Info

Product

Resources

About