Water-soluble N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride as a nucleic acids vector for cell transfection

Файзулоев, Е. Б.; Marova, Anna; Nikonova, Alexandra; Volkova, I. F.; Gorshkova, M. Yu.; Izumrudov, Vladimir A.

doi:10.1016/j.carbpol.2012.03.071

Cited by 44 publications

(27 citation statements)

References 27 publications

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“…Quaternization of chitosan has been performed to improve solubility at physiological pH. Two methods of quaternization [171] have been performed: N-trialkylation using halogenoalkanes (usually CH 3 I) [176][177][178][179][180] and a reaction with a quaternized epoxide [181][182][183].…”

Section: Modification On the C2 Aminementioning

confidence: 99%

“…To circumvent this problem, many types of chitosan derivatives have been synthesized with the goal of improving solubility and resulting colloidal stability of the polyplexes in physiological media. These include PEGylation [114,151,172], glycosylation [13,150,300] and trimethylation [114,151,179,180,183]. These modified chitosans have shown improvements in DNA protection and/ or TE in vitro at physiological pH, motivating in vivo studies to ascertain the advantages of such chitosans.…”

Section: Other Formulation Parameters Affecting Tementioning

confidence: 99%

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Chitosans for delivery of nucleic acids

Buschmann

Merzouki

Lavertu

et al. 2013

Advanced Drug Delivery Reviews

198

134

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Alternatives to efficient viral vectors in gene therapy are desired because of their poor safety profiles. Chitosan is a promising non-viral nucleotide delivery vector because of its biocompatibility, biodegradability, low immunogenicity and ease of manufacturing. Since the transfection efficiency of chitosan polyplexes is relatively low compared to viral counterparts, there is an impetus to gain a better understanding of the structure-performance relationship. Recent progress in preparation and characterisation has enabled coupling analysis of chitosans structural parameters that has led to increased TE by tailoring of chitosan's structure. In this review, we summarize the recent advances that have lead to a more rational design of chitosan polyplexes. We present an integrated review of all major areas of chitosan-based transfection, including preparation, chitosan and polyplexes physicochemical characterisation, in vitro and in vivo assessment. In each, we present the obstacles to efficient transfection and the strategies adopted over time to surmount these impediments.

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Section: Modification On the C2 Aminementioning

confidence: 99%

Section: Other Formulation Parameters Affecting Tementioning

confidence: 99%

Chitosans for delivery of nucleic acids

Buschmann

Merzouki

Lavertu

et al. 2013

Advanced Drug Delivery Reviews

198

134

View full text Add to dashboard Cite

show abstract

“…DOX was projected to release after cellular internalization of NCs at the acidic pH of endosomes due to hydrolysis of hydrazone linkage of DOX-CMP for cytoplasmic and nucleus accumulation to induce anticancer activity (Sang et al 2002). These cationic NCs could useful to bind with pDNA to escape from endosomes with the help of the ''proton sponge'' effect of cationic PAA for successful nuclear accumulation of pDNA and gene expression in target cells (Faizuloev et al 2012). In this work, anionic pullulan with conjugated DOX was successfully designed to form polyionic NCs with cationic PAA polymer first time for DNA delivery.…”

Section: Introductionmentioning

confidence: 97%

Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery

et al. 2014

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The amalgamation of chemotherapy and gene therapy is promising treatment option for cancer. In this study, novel biocompatible self-assembled nanocomplexes (NCs) between carboxylmethylated pullulan t335 (CMP) with polyallylamine (CMP-PAA NCs) were developed for plasmid DNA (pDNA) and pH-sensitive doxorubicin (DOX) delivery. DOX was conjugated to CMP (DOX-CMP) via hydrazone and confirmed by FTIR and 1 H-NMR. In vitro release studies of pH-sensitive DOX-CMP conjugate showed 23 and 85 % release after 48 h at pH 7.4 (physiological pH) and pH 5 (intracellular/tumoral pH), respectively. The CMP-PAA NCs or DOX-CMP-PAA NCs self-assembled into a nanosized (\250 nm) spherical shape as confirmed by DLS and TEM. The hemolysis and cytotoxicity study indicated that the CMP-PAA NCs did not show cytotoxicity in comparison with plain polyallylamine. Gel retardation assay showed complete binding of pDNA with CMP-PAA NCs at 1:2 weight ratio. CMP-PAA NCs/pDNA showed significantly higher transfection in HEK293 cells compared to PAA/pDNA complexes. Confocal imaging demonstrated successful cellular uptake of DOX-CMP-PAA NCs in HEK293 cells. Thus, NCs hold great potential for targeted pDNA and pH-sensitive intratumoral drug delivery.

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“…According to the different substitution sites of carboxymethyl groups, carboxymethyl chitosan can be divided into three types: N , O -carboxymethylated chitosan, O -carboxymethylated chitosan and N -carboxymethylated chitosan. Carboxymethyl chitosan biomaterials can be fabricated in neutral water medium, thus maintaining the activity of active substances such as small molecule drugs, proteins and cells, which expands the application range of chitosan [23,24,25,26]. Meanwhile, carboxymethyl chitosan possesses better sustained and controlled drug release performance [27,28,29,30].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings

2019

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As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings. Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing. Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application. In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking. The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates. Skin-like tensile performance allowed the dressings to provide a better healing environment. Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings. The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time. Cell culture assay showed that the dressings had excellent cell biocompatibility. As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.

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Water-soluble N-[(2-hydroxy-3-trimethylammonium)propyl]chitosan chloride as a nucleic acids vector for cell transfection

Cited by 44 publications

References 27 publications

Chitosans for delivery of nucleic acids

Chitosans for delivery of nucleic acids

Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery

Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings

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