Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity

Zheng, Zhaomin; Huang, Qilin; Ling, Chuqi

doi:10.1016/j.ijbiomac.2018.11.020

Cited by 63 publications

(21 citation statements)

References 54 publications

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“…Recent studies have found that polysaccharides can directly or indirectly act on the lower end of the intestine, and then regulate systemic immune response by changing the composition and abundance of intestinal microorganisms and promoting the production of short chain fatty acids. It was well known that most polysaccharides cannot be digested and absorbed in the stomach and small intestine, but they can be partially or completely hydrolyzed and metabolized into short chain fatty acids (SCFAs) by gut microbiota [ 41 ]. The important role of SCFAs is to provide energy for intestinal cells, promote the growth of intestinal cells, and regulate the innate immune system and adaptive immune system [ 40 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide

et al. 2021

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The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated. Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased. CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum. In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level. CMP-1 and CMP-2 could reduce the relative abundance Erysipelatoclostridum at the genus level. CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota. The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide

et al. 2021

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“…The polysaccharide was weighed and added to the TFA acid solution for continuous heating and hydrolysis for 2 h. After drying, it was put into a chromatographic bottle and eluted with a gradient of NaOH and NaOAc at a flow rate of 0.5 mL/min. The standard substance was determined by the same method [ 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

Hepatoprotective Polysaccharides from Geranium wilfordii: Purification, Structural Characterization, and Their Mechanism

Feng

Xie²,

Zhang³

et al. 2022

Molecules

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Traditional Chinese Medicine is generally used as a decoction to guard health. Many active ingredients in the decoction are chemical ingredients that are not usually paid attention to in phytochemical research, such as polysaccharides, etc. Based on research interest in Chinese herbal decoction, crude polysaccharides from G. wilfordii (GCP) were purified to obtain two relatively homogeneous polysaccharides, a neutral polysaccharide (GNP), and an acid polysaccharide (GAP) by various chromatographic separation methods, which were initially characterized by GC-MS, NMR, IR, and methylation analysis. Studies on the hepatoprotective activity of GCP in vivo showed that GCP might be a potential agent for the prevention and treatment of acute liver injury by inhibiting the secretion levels of ALT, AST, IL-6, IL-1β, TNF-α, and MDA expression levels, increasing SOD, and the GSH-Px activity value. Further, in vitro assays, GNP and GAP, decrease the inflammatory response by inhibiting the secretion of IL-6 and TNF-α, involved in the STAT1/T-bet signaling pathway.

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“…Furthermore, size exclusion separations of SEH and CEH were conducted using an ÄKTA Prime Plus system (GE Healthcare, Uppsala, Sweden) equipped with a Hiprep 26/ 60 Sephacryl S-400 column (GE Healthcare, Uppsala, Sweden). 23 The fractions were eluted with 0.2 mM NaCl and 50 mM PBS buffer ( pH 7.0). The levels of protein in all the effluents were determined by measuring the absorbance at 280 nm.…”

Section: Preparation Of N Haitanensis Enzymatic Hydrolysatesmentioning

confidence: 99%

The compound enzymatic hydrolysate of Neoporphyra haitanensis improved hyperglycemia and regulated the gut microbiome in high-fat diet-fed mice

Cheng

Jiang

et al. 2022

Food Funct.

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Water-soluble yeast β‑glucan fractions with different molecular weights: Extraction and separation by acidolysis assisted-size exclusion chromatography and their association with proliferative activity

Cited by 63 publications

References 54 publications

Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide

Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide

Hepatoprotective Polysaccharides from Geranium wilfordii: Purification, Structural Characterization, and Their Mechanism

The compound enzymatic hydrolysate of Neoporphyra haitanensis improved hyperglycemia and regulated the gut microbiome in high-fat diet-fed mice

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