Water structure and electron trapping in aqueous ionic solutions

Wolszczak, Marian; Wypych, M.; Tomczyk, Mariusz; Kroh, J.

doi:10.1163/15685670260373308

Cited by 2 publications

(2 citation statements)

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“…The three-dimensional structure of water is extensively studied not only in neat water but also in the presence of biologically important molecules e.g., proteins [ 35 ]. In liquid water, a network of H-bonds is established, resulting in a highly complex molecular structure.…”

Section: Resultsmentioning

confidence: 99%

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Spontaneous and Ionizing Radiation-Induced Aggregation of Human Serum Albumin: Dityrosine as a Fluorescent Probe

Radomska

Wolszczak

2022

IJMS

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The use of spectroscopic techniques has shown that human serum albumin (HSA) undergoes reversible self-aggregation through protein–protein interactions. It ensures the subsequent overlapping of electron clouds along with the stiffening of the conformation of the interpenetrating network of amino acids of adjacent HSA molecules. The HSA oxidation process related to the transfer of one electron was investigated by pulse radiolysis and photochemical methods. It has been shown that the irradiation of HSA solutions under oxidative stress conditions results in the formation of stable protein aggregates. The HSA aggregates induced by ionizing radiation are characterized by specific fluorescence compared to the emission of non-irradiated solutions. We assume that HSA dimers are mainly responsible for the new emission. Dityrosine produced by the intermolecular recombination of protein tyrosine radicals as a result of radiolysis of an aqueous solution of the protein is the main cause of HSA aggregation by cross-linking. Analysis of the oxidation process of HSA confirmed that the reaction of mild oxidants (Br2•−, N3•, SO4•−) with albumin leads to the formation of covalent bonds between tyrosine residues. In the case of •OH radicals and partly, Cl2•−, species other than DT are formed. The light emission of this species is similar to the emission of self-associated HSA.

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Section: Resultsmentioning

confidence: 99%

“…This structure leads to many overlapping bands in the IR spectrum. The four major water NIR absorption bands are centered near 970, 1200, 1450 and 1900 nm [ 35 ]. Strong near-infrared absorption bands of water near 1400–1440 nm have often been applied to a quantitative analysis of water content in foods and pharmaceuticals.…”

Section: Resultsmentioning

confidence: 99%