Water structure in nanopores of agarose gel by Raman spectroscopy

Ratajska-Gadomska, B.; Gadomski, W.

doi:10.1063/1.1826051

Cited by 53 publications

(44 citation statements)

References 28 publications

(20 reference statements)

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“…[217,218] Raman spectroscopy of the OH-stretching mode has been used to obtain information on the structure of water under a variety of different physical and chemical conditions. [35,219,220] In comparing maps and Raman spectra in Figures 29 and 31, one notices a bold difference in the spectral region 2900-3700 cm À1 when spectra are recorded inside MSCs, as compared with spectra collected at outside regions or within NMSCs or neuronal cells. Mapping the bold spectral feature centered at $3470 cm À1 perfectly matches maps built up with the hypotaurine fingerprint of 978 cm À1 (cf.…”

Section: In Situ Molecular Analyses Of Peripheral Nerve Myelinationmentioning

confidence: 99%

Raman spectroscopy in cell biology and microbiology

Pezzotti

2021

J Raman Spectroscopy

135

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The Raman spectrum of living cells and microorganisms contains highly specific fingerprint‐like signatures, useful in unequivocally identifying different species and interpreting physiological and metabolic responses to environmental stressors. In situ Raman imaging with dedicated highly sensitive instruments can translate selected spectroscopic fingerprints into vivid snapshots of molecular species or specific physiological reactions. Time‐lapse experiments, crucial in characterizing growth‐dependent phenomena and metabolic response to drugs or substrates, are possible because Raman imaging is life compatible. This review covers miscellaneous examples of Raman analyses and imaging of eukaryotic cells, bacteria, and viruses. Fundamental microbiological analyses covered here include (i) identification of different species of cells, bacteria, and viruses; (ii) characterization of the metabolic responses of cells and bacteria to different substrates; (iii) time‐lapse analyses of cell metabolic reactions upon viral inoculation; (iv) chemical imaging of axon sprouting in neuronal cells; and (v) visualization of the myelinating activity of living Schwann cells in coculture with neuronal cells. The spectroscopic findings displayed here, which are based on a machine learning approach applied to Raman analysis and imaging, demonstrate the invaluable potential for Raman spectroscopy in biophysics research.

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Section: In Situ Molecular Analyses Of Peripheral Nerve Myelinationmentioning

confidence: 99%

Raman spectroscopy in cell biology and microbiology

Pezzotti

2021

J Raman Spectroscopy

135

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“…The measure of the integral intensities of both components gives information of how the regular tetrahedral H-bonding network of can be disrupted. In this approach, several studies on bulk water and once confined in surfactant systems, have used the ratio of the component at about 3200 cm À1 to the component at about 3400 cm À1 , defined as R OH which represents the fraction of water in the system that takes part in the regular tetrahedral H-bonding network [22,25,[28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Confinement effects on water structure in membrane lyotropic phases

Guégan

2011

Journal of Colloid and Interface Science

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The change of the water structure in aqueous solutions of the tri-ethyleneglycol mono n-decyl ether (C(10)E(3)) was studied by micro Raman scattering. The results obtained on the O-H stretching band show that the behavior of the hydrogen bonding (H-bonds) water network can be used as a probe to follow the lamellar (L(α)) to sponge (L(3)) phase transition. In the lamellar phase, the stack of the surfactant molecules aggregated into a two-dimensional structure (membrane) acts as a soft confinement system for the H-bond water network of which the regular tetrahedral structure is perturbed. The change of the planar organization of the membranes to a highly disordered and infinite array of bilayers in the sponge phase amplifies the surface of contact between amphiphilic surfactant and water molecules which drives a strong disruption of the regular tetrahedral H-bonding water network.

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“…The interaction at the solid/liquid interface changes the structure of liquids nearby the interfaces 107, 108. For example, water in hydrogels has been studied109–114 and found to exhibit three distinct structures: bound water, free water, and intermediate water 115–117. The bound water interacts strongly with the solid network and has aggregation structures that are different from the bulk water.…”

Section: Introductionmentioning

confidence: 99%

Crystal Growth of Calcium Carbonate in Hydrogels as a Model of Biomineralization

Asenath‐Smith

Keene

et al. 2012

Adv Funct Materials

202

307

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A gel is defi ned as a two-component (solid and liquid), continuous, solid-like material with viscoelastic rheological Crystal Growth of Calcium Carbonate in Hydrogels as a Model of BiomineralizationIn recent years, the prevalence of hydrogel-like organic matrices in biomineralization has gained attention as a route to synthesizing a diverse range of crystalline structures. Here, examples of hydrogels in biological, as well as synthetic, bio-inspired systems are discussed. Particular attention is given to understanding the physical versus chemical effects of a broad range of hydrogel matrices and their role in directing polymorph selectivity and morphological control in the calcium carbonate system. Finally, recent data regarding hydrogel-matrix incorporation into the growing crystals is discussed and a mechanism for the formation of these single-crystal composite materials is presented. Future and is researching crystal growth in gels as a means to form nanocomposites.chains form helices (double or single helices) that subsequently aggregate into three-dimensional (3D) bundles, forming a porous network with fi brous characteristics (Figure 1 a,b). [ 82 , 83 ] Both the gelling and melting temperatures can be tailored by chemical modifi cation such as partial hydroxyethylation. [ 84 ] The mechanical behavior of agarose gels is sensitive to molecular weight and concentration [ 85 ] as well as chemical modifi cation.

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Water structure in nanopores of agarose gel by Raman spectroscopy

Cited by 53 publications

References 28 publications

Raman spectroscopy in cell biology and microbiology

Raman spectroscopy in cell biology and microbiology

Confinement effects on water structure in membrane lyotropic phases

Crystal Growth of Calcium Carbonate in Hydrogels as a Model of Biomineralization

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