Water yam (Dioscorea alata L.) diversity pattern in Brazil: an analysis with SSR and morphological markers

Siqueira, Marcos Vinícius Bohrer Monteiro; Bonatelli, Maria Letícia; Günther, Torsten; Gawenda, Inka; Schmid, Karl; Pavinato, Vitor Antonio Corrêa; Veasey, Elizabeth Ann

doi:10.1007/s10722-013-0063-4

Cited by 25 publications

(27 citation statements)

References 48 publications

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“…Regarding all 24 SSRs loci investigated, the estimated diversity index (H’ = 0.66) was found to be high. Genetic diversity detected in this study is much higher than the one reported by Siqueira et al [59] for 89 Brazilian D . alata cultivars (H’ = 0.41).…”

Section: Discussioncontrasting

confidence: 84%

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Arnau

Bhattacharjee

et al. 2017

PLoS ONE

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Yams (Dioscorea sp.) are staple food crops for millions of people in tropical and subtropical regions. Dioscorea alata, also known as greater yam, is one of the major cultivated species and most widely distributed throughout the tropics. Despite its economic and cultural importance, very little is known about its origin, diversity and genetics. As a consequence, breeding efforts for resistance to its main disease, anthracnose, have been fairly limited. The objective of this study was to contribute to the understanding of D. alata genetic diversity by genotyping 384 accessions from different geographical regions (South Pacific, Asia, Africa and the Caribbean), using 24 microsatellite markers. Diversity structuration was assessed via Principal Coordinate Analysis, UPGMA analysis and the Bayesian approach implemented in STRUCTURE. Our results revealed the existence of a wide genetic diversity and a significant structuring associated with geographic origin, ploidy levels and morpho-agronomic characteristics. Seventeen major groups of genetically close cultivars have been identified, including eleven groups of diploid cultivars, four groups of triploids and two groups of tetraploids. STRUCTURE revealed the existence of six populations in the diploid genetic pool and a few admixed cultivars. These results will be very useful for rationalizing D. alata genetic resources in breeding programs across different regions and for improving germplasm conservation methods.

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Section: Discussioncontrasting

confidence: 84%

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Arnau

Bhattacharjee

et al. 2017

PLoS ONE

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“…Therefore, these primers had adequate discriminatory power, allowing them to detect high polymorphism among the genotypes. Siqueira et al (2014) found an average of 0.92 for D. alata accessions, while Nascimento et al (2013) found a lower average value (0.79) for D. trifida accessions, but still higher than the values found in this study.…”

Section: Discussioncontrasting

confidence: 87%

“…Croxton et al (2011) analyzed two SSR loci among D. bulbifera accessions with different origins (USA, Africa, and China) and found two and four alleles for each loci, respectively, with an average of 3.0 alleles, which is similar to our study. Siqueira et al (2014) obtained an average of 5.1 alleles per locus among 89 D. alata accessions using 12 SSR loci. The average number of alleles per locus we found in D. bulbifera was also lower than the averages found by Hochu et al (2006), Tostain et al (2006), andObidiegwu et al (2009b), who found 7.6, 7.3, and 8.4 alleles per locus, respectively, in several species of Dioscorea.…”

Section: Discussionmentioning

confidence: 99%

“…Several SSR primers have been developed and used to assess the genetic diversity and structure of yam species, such as cultivated D. alata (Tostain et al, 2006;Sartie et al, 2012;Siqueira et al, 2011Siqueira et al, , 2014, D. trifida (Hochu et al, 2006;Nascimento et al, 2013), and D. cayenensis/D. rotundata (Tostain et al, 2007;Obidiegwu et al, 2009b;Sartie et al, 2012;Scarcelli et al, 2005Scarcelli et al, , 2013Mengesha et al, 2013;Silva et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity among air yam (Dioscorea bulbifera) varieties based on single sequence repeat markers

Silva¹,

Siqueira²,

Carrasco³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Dioscorea is the largest genus in the Dioscoreaceae family, and includes a number of economically important species including the air yam, D. bulbifera L. This study aimed to develop new single sequence repeat primers and characterize the genetic diversity of local varieties that originated in several municipalities of Brazil. We developed an enriched genomic library for D. bulbifera resulting in seven primers, six of which were polymorphic, and added four polymorphic loci developed for other Dioscorea species. This resulted in 10 polymorphic primers to evaluate 42 air yam accessions. Thirtythree alleles (bands) were found, with an average of 3.3 alleles per locus. The discrimination power ranged from 0.113 to 0.834, with an average of 0.595. Both principal coordinate and cluster analyses (using the Jaccard Index) failed to clearly separate the accessions according to their origins. However, the 13 accessions from Conceição dos Ouros, Minas Gerais State were clustered above zero on the principal coordinate 2 axis, and were also clustered into one subgroup in the cluster analysis. Accessions from Ubatuba, São Paulo State were clustered below zero on the same principal coordinate 2 axis, except for one accession, although they were scattered in several subgroups in the cluster analysis. Therefore, we found little spatial structure in the accessions, although those from Conceição dos Ouros and Ubatuba exhibited some spatial structure, and that there is a considerable level of genetic diversity in D. bulbifera maintained by traditional farmers in Brazil.

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“…Likewise, Benor et al (2012) also used capsule shape and weight characters in the genetic diversity analysis and characterization of Corchorus olitorius successfully. Recently, diversity in some other crops such as water yam, luffa and Corchrus spp have been reported based on different morphological characters (Benor et al, 2012;Prakash et al, 2013;Siqueira et al, 2014). Therefore, based on literature and our findings both fruit shapes and weight are very imperative traits that can successfully be used for the diversity assessment and characterization of mango germplasm.…”

Section: Discussionsupporting

confidence: 52%

MORPHOLOGICAL AND PHYSICO-CHEMICAL DIVERSITY IN SOME INDIGENOUS MANGO (Mangifera indica L.) GERMPLASM OF PAKISTAN

Raza¹

2017

PAKJAS

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Mango is the second major fruit crop of Pakistan. A large number of unexplored indigenous mango germplasm with great economic significance is present in the country. Hence, 425 mango accessions were studied from Azad Jammu & Kashmir (AJK) as well as Northern and Southern Punjab to explore the genetic diversity. Total 33 traits (25 qualitative and 8 quantitative) enabled the assessment of morphological and physico-chemical diversity of the studied indigenous mango germplasm. The first three principal components (PCs) contributed 68.06% variability among all mango accessions. The PCs also successfully grouped mango accessions according to their morphological and physico-chemical characteristics. Trunk height, tree circumference, crown diameter, leaf colour, leaf blade length, petiole length, inflorescence length, inflorescence width, fruit shape, fruit weight, soluble solid contents, titratable acidity, sugar:acid ratio, reducing sugars, non-reducing sugars and total sugars were found highly variable. Many of these characters are of substantial economic significance and could be used as breeding goals to increase the germplasm repository as well as fruit yield and quality. In conclusion, morphological and physico-chemical traits were highly useful for mango germplasm characterization. Several accessions also showed potentially good traits which could be used to develop new mango cultivars through future breeding schemes.

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Water yam (Dioscorea alata L.) diversity pattern in Brazil: an analysis with SSR and morphological markers

Cited by 25 publications

References 48 publications

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Genetic diversity among air yam (Dioscorea bulbifera) varieties based on single sequence repeat markers

MORPHOLOGICAL AND PHYSICO-CHEMICAL DIVERSITY IN SOME INDIGENOUS MANGO (Mangifera indica L.) GERMPLASM OF PAKISTAN

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