Waterborne exposure to PFOS causes disruption of the hypothalamus–pituitary–thyroid axis in zebrafish larvae

Shi, Xiongjie; Liu, Chunsheng; Wu, Guoqiao; Zhou, Bingsheng

doi:10.1016/j.chemosphere.2009.07.074

Cited by 200 publications

(100 citation statements)

References 71 publications

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“…In fish, T4 and T3 are the main form of THs secreted from the thyroid gland and plays major roles in the development and growth. It was reported that exposure to different concentrations of Polybrominated diphenyl ethers (PBDEs) and Perfluorooctane sulfonate (PFOS) could significantly alter T4 and T3 levels in zebrafish larvae accompanying with the body weight reduction (Yu et al, 2010(Yu et al, , 2011Shi et al, 2009). In this study, we observed that the average body length of zebrafish was significantly reduced by the exposure to MCLR which is in agreement with other studies, highlighting the interferences of MCLR with the growth of fish larvae (Oberemm et al, 1997;Wang et al, 2005;Li et al, 2011).…”

Section: Discussionsupporting

confidence: 81%

“…Thus, this observation is consistent with the results from the present study. The gene expression of NIS and TG has been reported to be sensitive to the THs levels and could be used as biomarkers to monitor thyroid activity during normal development (Manchado et al, 2008;Shi et al, 2009). Thus up-regulation of the mRNA expression of NIS and TG might indicate the MCLR-induce thyroid endocrine disruption in developing zebrafish larvae.…”

Section: Discussionmentioning

confidence: 99%

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Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis in zebrafish larvae

et al. 2012

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis in zebrafish larvae

et al. 2012

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“…Several nuclear receptors have been suggested to affect estrogenic gene expression. Among these are peroxisome proliferator-activated receptors (PPARs) and thyroid hormone receptors (TRs) that were affected by PFOS Fang et al, 2012;Shi et al, 2009;Shipley et al, 2004). The present findings are in accordance with other studies indicating estrogenic effects of PFOS in teleost species, demonstrating altered, and mostly elevated expression of estrogen responsive genes (Du et al, 2009;Fang et al, 2012;Keiter et al, 2012;Liu et al, 2007).…”

Section: Effects Of Pfossupporting

confidence: 82%

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

et al. 2014

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In the aquatic environments, the predicted changes in water temperature, pO2 and pCO2 could result in hypercapnic and hypoxic conditions in aquatic animals. These conditions are thought to affect several basic cellular and physiological mechanisms. Yet, possible adverse effects of elevated CO2 (hypercapnia) on teleost fish, as well as combined effects with emerging and legacy environmental contaminants are poorly investigated. In this study, juvenile Atlantic cod (Gadus morhua) were divided into groups and exposed to three different water bath PFOS exposure regimes (0 (control), 100 and 200 µg/L) for 5 days at 1hr/day, followed by three different CO2-levels (normocapnia, moderate (0.3%) and high (0.9%)). The moderate CO2 level is the predicted near future (within year 2300) level, while 0.9% represent severe hypercapnia. Tissue samples were collected at 3, 6 and 9 days after initiated CO2 exposure. Effects on the endocrine and biotransformation systems were examined by analyzing levels of sex steroid hormones (E2, T, 11-KT) and transcript expression of estrogen responsive genes (ERα, Vtg-α, Vtg-β, ZP2 and ZP3). In addition, transcripts for xenobiotic metabolizing enzymes (cyp1a and cyp3a) and hypoxiainducible factor (HIF-1α) were analyzed. Hypercapnia alone produced increased levels of sex steroid hormones (E2, T, 11-KT) with concomitant increase of estrogen responsive genes, while PFOS produced weak and time-dependent effects on E2-inducible gene transcription. Combined PFOS and hypercapnia exposure produced increased effects on sex steroid levels as observed for hypercapnia alone, with transcript expression patterns that are indicative of time-dependent interactive effects. Exposure to hypercapnia singly or in combination with PFOS produced modulations of the biotransformation and hypoxic responses that were apparently concentration and time-dependent. Loading plots of principal component analysis (PCA) produced a significant grouping of individual scores according to the exposure scenarios at day 6 and 9. Overall, the PCA analysis produced a unique clustering of variables that signifies a positive correlation between exposure to high PFOS concentration and expression of E2 responsive genes. Notably, this pattern was not evident for individuals exposed to PFOS concentrations in combination with elevated CO2 scenarios. To our knowledge, the present study is the first of its kind, to evaluate such effects using combined exposure to a perfluoroalkyl sulfonate and elevated levels of CO2 saturation, representative of future oceanic climate change, in any fish species or lower vertebrate.

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“…The concentration of DMSO in the water did not exceed 0.001% (v/v). During the experimental period, 50% of the exposure solution was renewed daily (Shi et al 2009). Water disposal from the aquaria was filtered through activated carbon before being delivered into the municipal sewage system.…”

Section: Experimental Design and Protocolmentioning

confidence: 99%

Temperature-dependent effects of PFOS on risk recognition and fast-start performance in juvenile Spinibarbus sinensis

Xia¹,

Ma²,

Guo³

et al. 2015

Aquat. Biol.

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Perfluorooctane sulfonate (PFOS) is a synthetic chemical substance that has become a ubiquitous environmental contaminant. It has been used in both industrial and consumer applications for over 50 yr, resulting in high levels of contamination worldwide. The potential ecotoxicity of PFOS has recently become a focus of interest and concern. The aim of this study was to investigate the impact of PFOS on risk recognition and escape performance of juvenile qingbo Spinibarbus sinensis. Fish were exposed to a range of PFOS concentrations (0, 0.32, 0.8, 2 and 5 mg l −1 ) at different temperatures (18 and 28°C) for 4 wk, at which point their antipredator behavior and fast-start swimming performance were assessed. We found that PFOS exposure caused qingbo to increase the time they spent in the 'risky area' (the area of the experimental aquarium closest to the predator tank) and reduce their average distance from the predator, as well as resulting in a noticeable increase in latency time and a significant decline in maximum linear velocity, maximum linear acceleration and escape distance. Many of these effects were more pronounced at the higher temperature. Our results indicate that exposure to PFOS could have deleterious effects on survival-related behavior in fish.

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Waterborne exposure to PFOS causes disruption of the hypothalamus–pituitary–thyroid axis in zebrafish larvae

Cited by 200 publications

References 71 publications

Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis in zebrafish larvae

Waterborne exposure to microcystin-LR alters thyroid hormone levels and gene transcription in the hypothalamic–pituitary–thyroid axis in zebrafish larvae

Effects of elevated dissolved carbon dioxide and perfluorooctane sulfonic acid, given singly and in combination, on steroidogenic and biotransformation pathways of Atlantic cod

Temperature-dependent effects of PFOS on risk recognition and fast-start performance in juvenile Spinibarbus sinensis

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